PDIA6 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/4

Western blot analysis of extracts from various samples, using PDIA6 Antibody.

DF12449 at 1/100 staining Human colorectal cancer and adjacent normal tissues by IHC-P.

DF12449 at 1/100 staining Human colorectal cancer by IHC-P.

DF12449 staining Hela by IF/ICC.

Product:	PDIA6 Antibody
Catalog:	DF12449
Description:	Rabbit polyclonal antibody to PDIA6
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	54 kDa; 48kD(Calculated).
Uniprot:	Q15084
RRID:	AB_2845254

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(93%), Horse(93%), Sheep(93%), Rabbit(93%), Dog(86%), Chicken(82%), Xenopus(93%)

Clonality:

Polyclonal

Specificity:

PDIA6 Antibody detects endogenous levels of total PDIA6.

RRID:

AB_2845254
Cite Format: Affinity Biosciences Cat# DF12449, RRID:AB_2845254.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Endoplasmic reticulum protein 5; ER protein 5; ERp5; P5; Pdia6; PDIA6_HUMAN; Protein disulfide isomerase A6; Protein disulfide isomerase associated 6; Protein disulfide isomerase family A member 6; Protein disulfide isomerase P5; Protein disulfide isomerase related protein; Protein disulfide-isomerase A6; Thioredoxin domain containing 7 (protein disulfide isomerase); Thioredoxin domain containing protein 7; Thioredoxin domain-containing protein 7; TXNDC7;

Immunogens

Immunogen:

Uniprot:

Q15084(PDIA6_HUMAN) >>Visit HPA database.

Gene(ID):

PDIA6(10130)

Expression:

Q15084 PDIA6_HUMAN:

Expressed in platelets (at protein level).

Sequence:

Q15084 PDIA6_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MALLVLGLVSCTFFLAVNGLYSSSDDVIELTPSNFNREVIQSDSLWLVEFYAPWCGHCQRLTPEWKKAATALKDVVKVGAVDADKHHSLGGQYGVQGFPTIKIFGSNKNRPEDYQGGRTGEAIVDAALSALRQLVKDRLGGRSGGYSSGKQGRSDSSSKKDVIELTDDSFDKNVLDSEDVWMVEFYAPWCGHCKNLEPEWAAAASEVKEQTKGKVKLAAVDATVNQVLASRYGIRGFPTIKIFQKGESPVDYDGGRTRSDIVSRALDLFSDNAPPPELLEIINEDIAKRTCEEHQLCVVAVLPHILDTGAAGRNSYLEVLLKLADKYKKKMWGWLWTEAGAQSELETALGIGGFGYPAMAAINARKMKFALLKGSFSEQGINEFLRELSFGRGSTAPVGGGAFPTIVEREPWDGRDGELPVEDDIDLSDVELDDLGKDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Bovine

Sheep

Xenopus

Rabbit

Dog

Chicken

Zebrafish

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15084 As Substrate

Q15084

Site	PTM Type	Enzyme	Source
S22	Phosphorylation	P68400 (CSNK2A1)	Uniprot
K66	Methylation		Uniprot
K73	Ubiquitination		Uniprot
K77	Ubiquitination		Uniprot
K85	Ubiquitination		Uniprot
Y93	Phosphorylation		Uniprot
K102	Acetylation		Uniprot
K102	Ubiquitination		Uniprot
K108	Ubiquitination		Uniprot
T119	Phosphorylation		Uniprot
S129	Phosphorylation		Uniprot
R142	Methylation		Uniprot
K150	Ubiquitination		Uniprot
S156	Phosphorylation		Uniprot
S158	Phosphorylation		Uniprot
K160	Ubiquitination		Uniprot
T166	Phosphorylation		Uniprot
K208	Ubiquitination		Uniprot
K216	Ubiquitination		Uniprot
S230	Phosphorylation		Uniprot
T239	Phosphorylation		Uniprot
K241	Acetylation		Uniprot
K241	Ubiquitination		Uniprot
K245	Ubiquitination		Uniprot
S248	Phosphorylation		Uniprot
Y252	Phosphorylation		Uniprot
S270	Phosphorylation		Uniprot
K288	Ubiquitination		Uniprot
S315	Phosphorylation		Uniprot
Y316	Phosphorylation		Uniprot
K368	Ubiquitination		Uniprot
K373	Ubiquitination		Uniprot
S375	Phosphorylation		Uniprot
S377	Phosphorylation		Uniprot
R392	Methylation		Uniprot
R409	Methylation		Uniprot
S428	Phosphorylation	P68400 (CSNK2A1)	Uniprot

Research Backgrounds

Q15084_PDIA6_HUMAN

Function:

May function as a chaperone that inhibits aggregation of misfolded proteins. Negatively regulates the unfolded protein response (UPR) through binding to UPR sensors such as ERN1, which in turn inactivates ERN1 signaling. May also regulate the UPR via the EIF2AK3 UPR sensor. Plays a role in platelet aggregation and activation by agonists such as convulxin, collagen and thrombin.

Subcellular Location:

Endoplasmic reticulum lumen. Cell membrane. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).

Tissue Specificity:

Expressed in platelets (at protein level).

Subunit Structure:

Part of a large chaperone multiprotein complex comprising DNAJB11, HSP90B1, HSPA5, HYOU, PDIA2, PDIA4, PDIA6, PPIB, SDF2L1, UGT1A1 and very small amounts of ERP29, but not, or at very low levels, CALR nor CANX. Interacts with MICA on the surface of tumor cells, leading to MICA disulfide bond reduction which is required for its release from tumor cells. Interacts with ITGB3 following platelet stimulation. Interacts with ERN1; the interaction is direct. Interacts with EIF2AK3.

Family&Domains:

Belongs to the protein disulfide isomerase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)

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PDIA6 Antibody - #DF12449