PDHX Antibody - #DF12448

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Product: PDHX Antibody
Catalog: DF12448
Description: Rabbit polyclonal antibody to PDHX
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Dog
Mol.Wt.: 54 kDa; 54kD(Calculated).
Uniprot: O00330
RRID: AB_2845253

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(83%), Bovine(83%), Horse(83%), Sheep(83%), Dog(83%)
Clonality:
Polyclonal
Specificity:
PDHX Antibody detects endogenous levels of total PDHX.
RRID:
AB_2845253
Cite Format: Affinity Biosciences Cat# DF12448, RRID:AB_2845253.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dihydrolipoamide dehydrogenase binding protein of pyruvate dehydrogenase complex; Dihydrolipoamide dehydrogenase-binding protein of pyruvate dehydrogenase complex; DLDBP; E3 binding protein; E3-binding protein; E3BP; Lipoyl containing pyruvate dehydrogenase complex component X; Lipoyl-containing pyruvate dehydrogenase complex component X; mitochondrial; ODPX_HUMAN; OPDX; PDHX; PDX 1; PDX1; Pro X; proX; Pyruvate dehydrogenase complex component X; Pyruvate dehydrogenase complex lipoyl containing component X; Pyruvate dehydrogenase complex, E3 binding protein subunit; Pyruvate dehydrogenase protein X component; Pyruvate dehydrogenase protein X component mitochondrial;

Immunogens

Immunogen:
Uniprot:

O00330(ODPX_HUMAN) >>Visit HPA database.

Gene(ID):
PDHX(8050)
Sequence:
MAASWRLGCDPRLLRYLVGFPGRRSVGLVKGALGWSVSRGANWRWFHSTQWLRGDPIKILMPSLSPTMEEGNIVKWLKKEGEAVSAGDALCEIETDKAVVTLDASDDGILAKIVVEEGSKNIRLGSLIGLIVEEGEDWKHVEIPKDVGPPPPVSKPSEPRPSPEPQISIPVKKEHIPGTLRFRLSPAARNILEKHSLDASQGTATGPRGIFTKEDALKLVQLKQTGKITESRPTPAPTATPTAPSPLQATAGPSYPRPVIPPVSTPGQPNAVGTFTEIPASNIRRVIAKRLTESKSTVPHAYATADCDLGAVLKVRQDLVKDDIKVSVNDFIIKAAAVTLKQMPDVNVSWDGEGPKQLPFIDISVAVATDKGLLTPIIKDAAAKGIQEIADSVKALSKKARDGKLLPEEYQGGSFSISNLGMFGIDEFTAVINPPQACILAVGRFRPVLKLTEDEEGNAKLQQRQLITVTMSSDSRVVDDELATRFLKSFKANLENPIRLA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Horse
83
Bovine
83
Sheep
83
Dog
83
Chicken
75
Xenopus
60
Zebrafish
50
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00330 As Substrate

Site PTM Type Enzyme
Y16 Phosphorylation
S25 Phosphorylation
S65 Phosphorylation
K79 Ubiquitination
T95 Phosphorylation
T101 Phosphorylation
K120 Acetylation
K120 Ubiquitination
S154 Phosphorylation
S162 Phosphorylation
S168 Phosphorylation
K173 Ubiquitination
T179 Phosphorylation
K194 Acetylation
S196 Phosphorylation
S200 Phosphorylation
R208 Methylation
K213 Ubiquitination
K223 Ubiquitination
K314 Ubiquitination
S364 Phosphorylation
T369 Phosphorylation
T375 Phosphorylation
K384 Ubiquitination
K394 Ubiquitination
K460 Ubiquitination
K488 Acetylation
K491 Acetylation
K491 Methylation

Research Backgrounds

Function:

Required for anchoring dihydrolipoamide dehydrogenase (E3) to the dihydrolipoamide transacetylase (E2) core of the pyruvate dehydrogenase complexes of eukaryotes. This specific binding is essential for a functional PDH complex.

PTMs:

Delipoylated at Lys-97 by SIRT4, delipoylation decreases the PHD complex activity.

Subcellular Location:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Part of the inner core of the multimeric pyruvate dehydrogenase complex that is composed of about 48 DLAT and 12 PDHX molecules. This core binds multiple copies of pyruvate dehydrogenase (subunits PDH1A and PDHB, E1), dihydrolipoamide acetyltransferase (DLAT, E2) and lipoamide dehydrogenase (DLD, E3). Interacts with SIRT4. Interacts with DLD.

Family&Domains:

Belongs to the 2-oxoacid dehydrogenase family.

Research Fields

· Metabolism > Global and overview maps > Metabolic pathways.

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