PDE6A Antibody | Affinity Biosciences

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Western blot analysis of extracts from various samples, using PDE6A Antibody.

Product:	PDE6A Antibody
Catalog:	DF12446
Description:	Rabbit polyclonal antibody to PDE6A
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	100-110 kDa; 100kD(Calculated).
Uniprot:	P16499
RRID:	AB_2845251

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(100%), Dog(92%)

Clonality:

Polyclonal

Specificity:

PDE6A Antibody detects endogenous levels of total PDE6A.

RRID:

AB_2845251
Cite Format: Affinity Biosciences Cat# DF12446, RRID:AB_2845251.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5''-cyclic phosphodiesterase subunit alpha; CGPR A; GMP PDE alpha; GMP-PDE alpha; PDE 6 alpha; PDE 6A; PDE V B1; PDE V-B1; PDE6A; PDE6A_HUMAN; PDEA; Phosphodiesterase 6 alpha; Phosphodiesterase 6A alpha subunit; Phosphodiesterase 6A cGMP specific rod alpha; Retinal Rod Photoreceptor cGMP Phosphodiesterase alpha; Rod cGMP specific 3' 5' cyclic phosphodiesterase alpha subunit; Rod cGMP-specific 3'';

Immunogens

Immunogen:

Uniprot:

P16499(PDE6A_HUMAN) >>Visit HPA database.

Gene(ID):

PDE6A(5145)

Sequence:

P16499 PDE6A_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGEVTAEEVEKFLDSNIGFAKQYYNLHYRAKLISDLLGAKEAAVDFSNYHSPSSMEESEIIFDLLRDFQENLQTEKCIFNVMKKLCFLLQADRMSLFMYRTRNGIAELATRLFNVHKDAVLEDCLVMPDQEIVFPLDMGIVGHVAHSKKIANVPNTEEDEHFCDFVDILTEYKTKNILASPIMNGKDVVAIIMAVNKVDGSHFTKRDEEILLKYLNFANLIMKVYHLSYLHNCETRRGQILLWSGSKVFEELTDIERQFHKALYTVRAFLNCDRYSVGLLDMTKQKEFFDVWPVLMGEVPPYSGPRTPDGREINFYKVIDYILHGKEDIKVIPNPPPDHWALVSGLPAYVAQNGLICNIMNAPAEDFFAFQKEPLDESGWMIKNVLSMPIVNKKEEIVGVATFYNRKDGKPFDEMDETLMESLTQFLGWSVLNPDTYESMNKLENRKDIFQDIVKYHVKCDNEEIQKILKTREVYGKEPWECEEEELAEILQAELPDADKYEINKFHFSDLPLTELELVKCGIQMYYELKVVDKFHIPQEALVRFMYSLSKGYRKITYHNWRHGFNVGQTMFSLLVTGKLKRYFTDLEALAMVTAAFCHDIDHRGTNNLYQMKSQNPLAKLHGSSILERHHLEFGKTLLRDESLNIFQNLNRRQHEHAIHMMDIAIIATDLALYFKKRTMFQKIVDQSKTYESEQEWTQYMMLEQTRKEIVMAMMMTACDLSAITKPWEVQSQVALLVAAEFWEQGDLERTVLQQNPIPMMDRNKADELPKLQVGFIDFVCTFVYKEFSRFHEEITPMLDGITNNRKEWKALADEYDAKMKVQEEKKQKQQSAKSAAAGNQPGGNPSPGGATTSKSCCIQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Bovine

100

Sheep

100

Rabbit

100

Horse

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P16499 As Substrate

P16499

Site	PTM Type	Source
K84	Methylation	Uniprot
S180	Phosphorylation	Uniprot
Y264	Phosphorylation	Uniprot
Y275	Phosphorylation	Uniprot
T283	Phosphorylation	Uniprot
K467	Acetylation	Uniprot
K677	Acetylation	Uniprot
T717	Phosphorylation	Uniprot

Research Backgrounds

P16499_PDE6A_HUMAN

Function:

This protein participates in processes of transmission and amplification of the visual signal.

Subcellular Location:

Cell membrane>Lipid-anchor>Cytoplasmic side.

Subunit Structure:

Oligomer composed of two catalytic chains (alpha and beta), an inhibitory chain (gamma) and the delta chain.

Family&Domains:

Belongs to the cyclic nucleotide phosphodiesterase family.

Research Fields

· Metabolism > Nucleotide metabolism > Purine metabolism.

· Organismal Systems > Sensory system > Phototransduction.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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PDE6A Antibody - #DF12446