Product: PAFAH2 Antibody
Catalog: DF12442
Description: Rabbit polyclonal antibody to PAFAH2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Dog
Mol.Wt.: 44-46 kDa; 44kD(Calculated).
Uniprot: Q99487
RRID: AB_2845247

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Horse(100%), Dog(89%)
Clonality:
Polyclonal
Specificity:
PAFAH2 Antibody detects endogenous levels of total PAFAH2.
RRID:
AB_2845247
Cite Format: Affinity Biosciences Cat# DF12442, RRID:AB_2845247.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EC 3.1.1.47; HSD PLA2; HSDPLA2; Platelet activating factor acetylhydrolase 2; Platelet activating factor acetylhydrolase 2 cytoplasmic; platelet-activating factor acetylhydrolase 2, 40kDa; SD PLA2; Serine dependent phospholipase A2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q99487 PAFA2_HUMAN:

Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

Sequence:
MGVNQSVGFPPVTGPHLVGCGDVMEGQNLQGSFFRLFYPCQKAEETMEQPLWIPRYEYCTGLAEYLQFNKRCGGLLFNLAVGSCRLPVSWNGPFKTKDSGYPLIIFSHGLGAFRTLYSAFCMELASRGFVVAVPEHRDRSAATTYFCKQAPEENQPTNESLQEEWIPFRRVEEGEKEFHVRNPQVHQRVSECLRVLKILQEVTAGQTVFNILPGGLDLMTLKGNIDMSRVAVMGHSFGGATAILALAKETQFRCAVALDAWMFPLERDFYPKARGPVFFINTEKFQTMESVNLMKKICAQHEQSRIITVLGSVHRSQTDFAFVTGNLIGKFFSTETRGSLDPYEGQEVMVRAMLAFLQKHLDLKEDYNQWNNLIEGIGPSLTPGAPHHLSSL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
89
Dog
89
Bovine
78
Sheep
78
Rabbit
78
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99487 As Substrate

Site PTM Type Enzyme
Y101 Phosphorylation
K148 Ubiquitination
K176 Acetylation
K176 Ubiquitination
K330 Ubiquitination
K364 Ubiquitination

Research Backgrounds

Function:

Has a marked selectivity for phospholipids with short acyl chains at the sn-2 position. May share a common physiologic function with the plasma-type enzyme.

Subcellular Location:

Cytoplasm. Membrane>Lipid-anchor. Endoplasmic reticulum membrane>Lipid-anchor.
Note: In resting cells, localizes to intracellular membranes and cytoplasm. Translocates from the cytoplasm to intracellular membranes upon oxidative stress.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Broadly expressed in different tissues, but high in B- and T-lymphocytes. In brain, expression is restricted to amygdala and frontal cortex.

Subunit Structure:

Monomer.

Family&Domains:

Belongs to the serine esterase family.

Research Fields

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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