P4HA2 Antibody - #DF12440

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Product: P4HA2 Antibody
Catalog: DF12440
Description: Rabbit polyclonal antibody to P4HA2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 61 kDa; 61kD(Calculated).
Uniprot: O15460
RRID: AB_2845245

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Horse(100%), Sheep(100%), Rabbit(83%), Dog(100%)
Clonality:
Polyclonal
Specificity:
P4HA2 Antibody detects endogenous levels of total P4HA2.
RRID:
AB_2845245
Cite Format: Affinity Biosciences Cat# DF12440, RRID:AB_2845245.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2-oxoglutarate-4-dioxygenase subunit alpha-2; 4 PH alpha 2; 4-PH alpha-2; C-P4Halpha(II); collagen prolyl 4-hydroxylase alpha(II); P4HA2; P4HA2_HUMAN; procollagen proline, 2 oxoglutarate 4 dioxygenase (proline 4 hydroxylase), alpha polypeptide II; procollagen proline, 2 oxoglutarate 4 dioxygenase alpha subunit, isoform 2; Procollagen-proline; procollagen-proline,2-oxoglutarate-4-dioxygenase subunit alpha-2; Prolyl 4 hydroxylase alpha 2 subunit; Prolyl 4 hydroxylase subunit alpha 2 [Precursor]; Prolyl 4-hydroxylase subunit alpha-2; Prolyl 4-hydroxylase, alpha polypeptide II;

Immunogens

Immunogen:
Uniprot:

O15460(P4HA2_HUMAN) >>Visit HPA database.

Gene(ID):
P4HA2(8974)
Sequence:
MKLWVSALLMAWFGVLSCVQAEFFTSIGHMTDLIYAEKELVQSLKEYILVEEAKLSKIKSWANKMEALTSKSAADAEGYLAHPVNAYKLVKRLNTDWPALEDLVLQDSAAGFIANLSVQRQFFPTDEDEIGAAKALMRLQDTYRLDPGTISRGELPGTKYQAMLSVDDCFGMGRSAYNEGDYYHTVLWMEQVLKQLDAGEEATTTKSQVLDYLSYAVFQLGDLHRALELTRRLLSLDPSHERAGGNLRYFEQLLEEEREKTLTNQTEAELATPEGIYERPVDYLPERDVYESLCRGEGVKLTPRRQKRLFCRYHHGNRAPQLLIAPFKEEDEWDSPHIVRYYDVMSDEEIERIKEIAKPKLARATVRDPKTGVLTVASYRVSKSSWLEEDDDPVVARVNRRMQHITGLTVKTAELLQVANYGVGGQYEPHFDFSRNDERDTFKHLGTGNRVATFLNYMSDVEAGGATVFPDLGAAIWPKKGTAVFWYNLLRSGEGDYRTRHAACPVLVGCKWVSNKWFHERGQEFLRPCGSTEVD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
83
Chicken
75
Xenopus
70
Zebrafish
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15460 As Substrate

Site PTM Type Enzyme
T31 Phosphorylation
Y35 Phosphorylation
S43 Phosphorylation
K45 Ubiquitination
K54 Ubiquitination
K59 Ubiquitination
K64 Ubiquitination
K71 Ubiquitination
Y79 Phosphorylation
K88 Ubiquitination
K134 Ubiquitination
K194 Ubiquitination
K206 Ubiquitination
K300 Ubiquitination
K328 Sumoylation
K328 Ubiquitination
S346 Phosphorylation
K383 Ubiquitination
Y421 Phosphorylation
T441 Phosphorylation
K443 Ubiquitination
T447 Phosphorylation
T482 Phosphorylation
Y487 Phosphorylation

Research Backgrounds

Function:

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Subcellular Location:

Endoplasmic reticulum lumen.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI).

Family&Domains:

Belongs to the P4HA family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

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For Research Use Only.
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