Product: NSDHL Antibody
Catalog: DF12432
Description: Rabbit polyclonal antibody to NSDHL
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 42 kDa,40 kDa; 42kD(Calculated).
Uniprot: Q15738
RRID: AB_2845237

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(92%), Horse(83%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
NSDHL Antibody detects endogenous levels of total NSDHL.
RRID:
AB_2845237
Cite Format: Affinity Biosciences Cat# DF12432, RRID:AB_2845237.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

decarboxylating; H105E3; H105e3 protein; NAD(P) dependent steroid dehydrogenase like; NSDHL; NSDHL_HUMAN; Protein H105e3; SDR31E1; Short chain dehydrogenase/reductase family 31E member 1; Sterol 4 alpha carboxylate 3 dehydrogenase decarboxylating; Sterol-4-alpha-carboxylate 3-dehydrogenase; XAP104;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q15738 NSDHL_HUMAN:

Brain, heart, liver, lung, kidney, skin and placenta.

Sequence:
MEPAVSEPMRDQVARTHLTEDTPKVNADIEKVNQNQAKRCTVIGGSGFLGQHMVEQLLARGYAVNVFDIQQGFDNPQVRFFLGDLCSRQDLYPALKGVNTVFHCASPPPSSNNKELFYRVNYIGTKNVIETCKEAGVQKLILTSSASVIFEGVDIKNGTEDLPYAMKPIDYYTETKILQERAVLGANDPEKNFLTTAIRPHGIFGPRDPQLVPILIEAARNGKMKFVIGNGKNLVDFTFVENVVHGHILAAEQLSRDSTLGGKAFHITNDEPIPFWTFLSRILTGLNYEAPKYHIPYWVAYYLALLLSLLVMVISPVIQLQPTFTPMRVALAGTFHYYSCERAKKAMGYQPLVTMDDAMERTVQSFRHLRRVK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Zebrafish
92
Chicken
92
Horse
83
Xenopus
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q15738 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T19 Phosphorylation
T22 Phosphorylation
K24 Ubiquitination
K31 Ubiquitination
S87 Phosphorylation
K96 Ubiquitination
S106 Phosphorylation
K114 Ubiquitination
K126 Ubiquitination
K133 Ubiquitination
S147 Phosphorylation
Y164 Phosphorylation
K167 Ubiquitination
K176 Ubiquitination
K191 Ubiquitination
K232 Ubiquitination
K263 Ubiquitination
T284 Phosphorylation
Y288 Phosphorylation
K345 Ubiquitination
Y349 Phosphorylation
T354 Phosphorylation

Research Backgrounds

Function:

Involved in the sequential removal of two C-4 methyl groups in post-squalene cholesterol biosynthesis.

Subcellular Location:

Endoplasmic reticulum membrane>Single-pass membrane protein. Lipid droplet.
Note: Trafficking through the Golgi is necessary for ER membrane localization.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain, heart, liver, lung, kidney, skin and placenta.

Family&Domains:

Belongs to the 3-beta-HSD family.

Research Fields

· Metabolism > Lipid metabolism > Steroid biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.