NCKAP1 Antibody - #DF12428

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Product: NCKAP1 Antibody
Catalog: DF12428
Description: Rabbit polyclonal antibody to NCKAP1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 115-125 kDa; 129kD(Calculated).
Uniprot: Q9Y2A7
RRID: AB_2845233

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(92%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
NCKAP1 Antibody detects endogenous levels of total NCKAP1.
RRID:
AB_2845233
Cite Format: Affinity Biosciences Cat# DF12428, RRID:AB_2845233.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ11291; KIAA0587; Membrane associated protein HEM2; Membrane-associated protein HEM-2; MGC8981; NAP 1; NAP1; NAP125; NCK associated protein 1; NCK associated protein; Nck-associated protein 1; Nckap1; NCKP1_HUMAN; OTTHUMP00000163460; OTTHUMP00000163461; p125Nap1;

Immunogens

Immunogen:
Uniprot:

Q9Y2A7(NCKP1_HUMAN) >>Visit HPA database.

Gene(ID):
NCKAP1(10787)
Expression:
Q9Y2A7 NCKP1_HUMAN:

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Sequence:
MSRSVLQPSQQKLAEKLTILNDRGVGMLTRLYNIKKACGDPKAKPSYLIDKNLESAVKFIVRKFPAVETRNNNQQLAQLQKEKSEILKNLALYYFTFVDVMEFKDHVCELLNTIDVCQVFFDITVNFDLTKNYLDLIITYTTLMILLSRIEERKAIIGLYNYAHEMTHGASDREYPRLGQMIVDYENPLKKMMEEFVPHSKSLSDALISLQMVYPRRNLSADQWRNAQLLSLISAPSTMLNPAQSDTMPCEYLSLDAMEKWIIFGFILCHGILNTDATALNLWKLALQSSSCLSLFRDEVFHIHKAAEDLFVNIRGYNKRINDIRECKEAAVSHAGSMHRERRKFLRSALKELATVLSDQPGLLGPKALFVFMALSFARDEIIWLLRHADNMPKKSADDFIDKHIAELIFYMEELRAHVRKYGPVMQRYYVQYLSGFDAVVLNELVQNLSVCPEDESIIMSSFVNTMTSLSVKQVEDGEVFDFRGMRLDWFRLQAYTSVSKASLGLADHRELGKMMNTIIFHTKMVDSLVEMLVETSDLSIFCFYSRAFEKMFQQCLELPSQSRYSIAFPLLCTHFMSCTHELCPEERHHIGDRSLSLCNMFLDEMAKQARNLITDICTEQCTLSDQLLPKHCAKTISQAVNKKSKKQTGKKGEPEREKPGVESMRKNRLVVTNLDKLHTALSELCFSINYVPNMVVWEHTFTPREYLTSHLEIRFTKSIVGMTMYNQATQEIAKPSELLTSVRAYMTVLQSIENYVQIDITRVFNNVLLQQTQHLDSHGEPTITSLYTNWYLETLLRQVSNGHIAYFPAMKAFVNLPTENELTFNAEEYSDISEMRSLSELLGPYGMKFLSESLMWHISSQVAELKKLVVENVDVLTQMRTSFDKPDQMAALFKRLSSVDSVLKRMTIIGVILSFRSLAQEALRDVLSYHIPFLVSSIEDFKDHIPRETDMKVAMNVYELSSAAGLPCEIDPALVVALSSQKSENISPEEEYKIACLLMVFVAVSLPTLASNVMSQYSPAIEGHCNNIHCLAKAINQIAAALFTIHKGSIEDRLKEFLALASSSLLKIGQETDKTTTRNRESVYLLLDMIVQESPFLTMDLLESCFPYVLLRNAYHAVYKQSVTSSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
92
Xenopus
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2A7 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S4 Phosphorylation
K12 Ubiquitination
K16 Ubiquitination
T18 Phosphorylation
R23 Methylation
T29 Phosphorylation
K51 Ubiquitination
K58 Ubiquitination
K63 Ubiquitination
K81 Ubiquitination
T96 Phosphorylation
T141 Phosphorylation
T142 Phosphorylation
S148 Phosphorylation
S171 Phosphorylation
Y175 Phosphorylation
K190 Ubiquitination
K201 Acetylation
S337 Phosphorylation
K351 Ubiquitination
K501 Ubiquitination
S528 Phosphorylation
K551 Ubiquitination
K635 Ubiquitination
S638 Phosphorylation
K643 Ubiquitination
K644 Ubiquitination
S645 Phosphorylation
K651 Ubiquitination
S664 Phosphorylation
Y691 Phosphorylation
S719 Phosphorylation
S801 Phosphorylation
S838 Phosphorylation
S840 Phosphorylation
S852 Phosphorylation
K886 Ubiquitination
K895 Ubiquitination
S899 Phosphorylation
S902 Phosphorylation
T908 Phosphorylation
T1045 Phosphorylation
S1050 Phosphorylation
S1065 Phosphorylation
K1075 Ubiquitination
Y1116 Phosphorylation
Y1120 Phosphorylation
S1123 Phosphorylation

Research Backgrounds

Function:

Part of the WAVE complex that regulates lamellipodia formation. The WAVE complex regulates actin filament reorganization via its interaction with the Arp2/3 complex. Actin remodeling activity is regulated by RAC1. As component of the WAVE1 complex, required for BDNF-NTRK2 endocytic trafficking and signaling from early endosomes.

Subcellular Location:

Cell membrane>Single-pass membrane protein>Cytoplasmic side. Cell projection>Lamellipodium membrane>Single-pass membrane protein>Cytoplasmic side.
Note: At the interface between the lamellipodial actin meshwork and the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined except peripheral blood leukocytes, with highest expression in brain, heart, and skeletal muscle.

Subunit Structure:

Component of the WAVE1 complex composed of ABI2, CYFIP1 or CYFIP2, BRK1, NCKAP1 and WASF1/WAVE1. Within the complex, a heterodimer containing NCKAP1 and CYFIP1 interacts with a heterotrimer formed by WAVE1, ABI2 and BRK1. Component of the WAVE2 complex composed of ABI1, CYFIP1/SRA1, NCKAP1/NAP1 and WASF2/WAVE2. CYFIP2 binds to activated RAC1 which causes the complex to dissociate, releasing activated WASF1. The complex can also be activated by NCK1. Associates preferentially with the first SH3 domain of NCK. Interacts with NYAP1, NYAP2 and MYO16 (By similarity).

(Microbial infection) Interacts with human cytomegalovirus protein UL135.

Family&Domains:

Belongs to the HEM-1/HEM-2 family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

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