MYH6 Antibody - #DF12426

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Product: MYH6 Antibody
Catalog: DF12426
Description: Rabbit polyclonal antibody to MYH6
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Rabbit
Mol.Wt.: 200-220 kDa; 224kD(Calculated).
Uniprot: P13533
RRID: AB_2845231

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(100%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
MYH6 Antibody detects endogenous levels of total MYH6.
RRID:
AB_2845231
Cite Format: Affinity Biosciences Cat# DF12426, RRID:AB_2845231.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cardiac muscle alpha isoform;MYH6;MYH6_HUMAN;MYHC;MyHC-alpha;MyHC-beta;MYHCA;MYHCB;Myosin heavy chain 6;Myosin heavy chain;Myosin heavy chain cardiac muscle alpha isoform;Myosin heavy chain cardiac muscle beta isoform;Myosin-6;

Immunogens

Immunogen:
Uniprot:

P13533(MYH6_HUMAN) >>Visit HPA database.

Gene(ID):
MYH6(4624)
Sequence:
MTDAQMADFGAAAQYLRKSEKERLEAQTRPFDIRTECFVPDDKEEFVKAKILSREGGKVIAETENGKTVTVKEDQVLQQNPPKFDKIEDMAMLTFLHEPAVLFNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNAEVVAAYRGKKRSEAPPHIFSISDNAYQYMLTDRENQSILITGESGAGKTVNTKRVIQYFASIAAIGDRGKKDNANANKGTLEDQIIQANPALEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVIFQLKAERNYHIFYQILSNKKPELLDMLLVTNNPYDYAFVSQGEVSVASIDDSEELMATDSAFDVLGFTSEEKAGVYKLTGAIMHYGNMKFKQKQREEQAEPDGTEDADKSAYLMGLNSADLLKGLCHPRVKVGNEYVTKGQSVQQVYYSIGALAKAVYEKMFNWMVTRINATLETKQPRQYFIGVLDIAGFEIFDFNSFEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWTFIDFGMDLQACIDLIEKPMGIMSILEEECMFPKATDMTFKAKLYDNHLGKSNNFQKPRNIKGKQEAHFSLIHYAGTVDYNILGWLEKNKDPLNETVVALYQKSSLKLMATLFSSYATADTGDSGKSKGGKKKGSSFQTVSALHRENLNKLMTNLRTTHPHFVRCIIPNERKAPGVMDNPLVMHQLRCNGVLEGIRICRKGFPNRILYGDFRQRYRILNPVAIPEGQFIDSRKGTEKLLSSLDIDHNQYKFGHTKVFFKAGLLGLLEEMRDERLSRIITRMQAQARGQLMRIEFKKIVERRDALLVIQWNIRAFMGVKNWPWMKLYFKIKPLLKSAETEKEMATMKEEFGRIKETLEKSEARRKELEEKMVSLLQEKNDLQLQVQAEQDNLNDAEERCDQLIKNKIQLEAKVKEMNERLEDEEEMNAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDEIIAKLTKEKKALQEAHQQALDDLQVEEDKVNSLSKSKVKLEQQVDDLEGSLEQEKKVRMDLERAKRKLEGDLKLTQESIMDLENDKLQLEEKLKKKEFDINQQNSKIEDEQVLALQLQKKLKENQARIEELEEELEAERTARAKVEKLRSDLSRELEEISERLEEAGGATSVQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLELDDVTSNMEQIIKAKANLEKVSRTLEDQANEYRVKLEEAQRSLNDFTTQRAKLQTENGELARQLEEKEALISQLTRGKLSYTQQMEDLKRQLEEEGKAKNALAHALQSARHDCDLLREQYEEETEAKAELQRVLSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEAVEAVNAKCSSLEKTKHRLQNEIEDLMVDVERSNAAAAALDKKQRNFDKILAEWKQKYEESQSELESSQKEARSLSTELFKLKNAYEESLEHLETFKRENKNLQEEISDLTEQLGEGGKNVHELEKVRKQLEVEKLELQSALEEAEASLEHEEGKILRAQLEFNQIKAEIERKLAEKDEEMEQAKRNHQRVVDSLQTSLDAETRSRNEVLRVKKKMEGDLNEMEIQLSHANRMAAEAQKQVKSLQSLLKDTQIQLDDAVRANDDLKENIAIVERRNNLLQAELEELRAVVEQTERSRKLAEQELIETSERVQLLHSQNTSLINQKKKMESDLTQLQSEVEEAVQECRNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTIKDLQHRLDEAEQIALKGGKKQLQKLEARVRELEGELEAEQKRNAESVKGMRKSERRIKELTYQTEEDKKNLLRLQDLVDKLQLKVKAYKRQAEEAEEQANTNLSKFRKVQHELDEAEERADIAESQVNKLRAKSRDIGAKQKMHDEE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Bovine
92
Zebrafish
73
Xenopus
64
Pig
0
Sheep
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13533 As Substrate

Site PTM Type Enzyme
T2 Phosphorylation
Y162 Phosphorylation
Y164 Phosphorylation
T167 Phosphorylation
S173 Phosphorylation
K247 Ubiquitination
T256 Phosphorylation
S261 Phosphorylation
Y267 Phosphorylation
K271 Ubiquitination
T354 Phosphorylation
Y360 Phosphorylation
T379 Phosphorylation
Y387 Phosphorylation
Y411 Phosphorylation
T413 Phosphorylation
K414 Acetylation
Y422 Phosphorylation
Y423 Phosphorylation
T442 Phosphorylation
T447 Phosphorylation
K503 Acetylation
K550 Acetylation
Y554 Phosphorylation
S650 Phosphorylation
T662 Phosphorylation
T666 Phosphorylation
T667 Phosphorylation
Y717 Phosphorylation
Y758 Phosphorylation
K759 Acetylation
S784 Phosphorylation
S881 Phosphorylation
T939 Phosphorylation
K941 Acetylation
S950 Phosphorylation
K953 Acetylation
K954 Acetylation
T962 Phosphorylation
T981 Phosphorylation
T995 Phosphorylation
S1039 Phosphorylation
K1044 Acetylation
K1045 Acetylation
K1054 Acetylation
S1067 Phosphorylation
T1129 Phosphorylation
S1139 Phosphorylation
S1142 Phosphorylation
S1160 Phosphorylation
K1167 Acetylation
T1184 Phosphorylation
T1190 Phosphorylation
S1201 Phosphorylation
S1224 Phosphorylation
T1234 Phosphorylation
S1235 Phosphorylation
Y1261 Phosphorylation
S1301 Phosphorylation
T1304 Phosphorylation
S1309 Phosphorylation
Y1310 Phosphorylation
T1311 Phosphorylation
K1318 Acetylation
S1337 Phosphorylation
Y1349 Phosphorylation
T1353 Phosphorylation
S1364 Phosphorylation
S1368 Phosphorylation
T1375 Phosphorylation
Y1377 Phosphorylation
T1379 Phosphorylation
T1385 Phosphorylation
K1392 Acetylation
K1393 Acetylation
K1394 Acetylation
K1446 Acetylation
K1447 Acetylation
Y1462 Phosphorylation
S1465 Phosphorylation
S1467 Phosphorylation
S1471 Phosphorylation
S1472 Phosphorylation
S1478 Phosphorylation
S1480 Phosphorylation
T1481 Phosphorylation
Y1490 Phosphorylation
S1493 Phosphorylation
T1499 Phosphorylation
K1501 Acetylation
K1589 Acetylation
S1598 Phosphorylation
T1601 Phosphorylation
S1602 Phosphorylation
S1609 Phosphorylation
S1632 Phosphorylation
S1647 Phosphorylation
S1650 Phosphorylation
S1712 Phosphorylation
S1720 Phosphorylation
T1723 Phosphorylation
S1724 Phosphorylation
K1729 Acetylation
T1762 Phosphorylation
K1772 Acetylation
K1786 Acetylation
K1808 Acetylation
K1811 Acetylation
K1812 Acetylation
K1833 Acetylation
T1853 Phosphorylation
Y1854 Phosphorylation
K1860 Acetylation
T1893 Phosphorylation
S1896 Phosphorylation
S1917 Phosphorylation

Research Backgrounds

Function:

Muscle contraction.

Subcellular Location:

Cytoplasm>Myofibril.
Note: Thick filaments of the myofibrils.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Muscle myosin is a hexameric protein that consists of 2 heavy chain subunits (MHC), 2 alkali light chain subunits (MLC) and 2 regulatory light chain subunits (MLC-2).

Family&Domains:

The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.

Limited proteolysis of myosin heavy chain produces 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). HMM can be further cleaved into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

Research Fields

· Human Diseases > Cardiovascular diseases > Hypertrophic cardiomyopathy (HCM).

· Human Diseases > Cardiovascular diseases > Dilated cardiomyopathy (DCM).

· Human Diseases > Cardiovascular diseases > Viral myocarditis.

· Organismal Systems > Circulatory system > Cardiac muscle contraction.   (View pathway)

· Organismal Systems > Circulatory system > Adrenergic signaling in cardiomyocytes.   (View pathway)

· Organismal Systems > Endocrine system > Thyroid hormone signaling pathway.   (View pathway)

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