LRPPRC Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/3

Western blot analysis of extracts from Sp2/0, using LRPPRC Antibody.

DF12421 at 1/100 staining Mouse heart tissue by IHC-P.

Product:	LRPPRC Antibody
Catalog:	DF12421
Description:	Rabbit polyclonal antibody to LRPPRC
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	130 kDa; 158kD(Calculated).
Uniprot:	P42704
RRID:	AB_2845226

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(92%)

Clonality:

Polyclonal

Specificity:

LRPPRC Antibody detects endogenous levels of total LRPPRC.

RRID:

AB_2845226
Cite Format: Affinity Biosciences Cat# DF12421, RRID:AB_2845226.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

130 kDa leucine-rich protein; gp130; Leucine-rich PPR motif-containing protein; Leucine-rich PPR-motif containing protein; LPPRC_HUMAN; LRP 130; lrpprc; mitochondrial;

Immunogens

Immunogen:

Uniprot:

P42704(LPPRC_HUMAN) >>Visit HPA database.

Gene(ID):

LRPPRC(10128)

Expression:

P42704 LPPRC_HUMAN:

Expressed ubiquitously. Expression is highest in heart, skeletal muscle, kidney and liver, intermediate in brain, non-mucosal colon, spleen and placenta, and lowest in small intestine, thymus, lung and peripheral blood leukocytes.

Sequence:

P42704 LPPRC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAALLRSARWLLRAGAAPRLPLSLRLLPGGPGRLHAASYLPAARAGPVAGGLLSPARLYAIAAKEKDIQEESTFSSRKISNQFDWALMRLDLSVRRTGRIPKKLLQKVFNDTCRSGGLGGSHALLLLRSCGSLLPELKLEERTEFAHRIWDTLQKLGAVYDVSHYNALLKVYLQNEYKFSPTDFLAKMEEANIQPNRVTYQRLIASYCNVGDIEGASKILGFMKTKDLPVTEAVFSALVTGHARAGDMENAENILTVMRDAGIEPGPDTYLALLNAYAEKGDIDHVKQTLEKVEKSELHLMDRDLLQIIFSFSKAGYPQYVSEILEKVTCERRYIPDAMNLILLLVTEKLEDVALQILLACPVSKEDGPSVFGSFFLQHCVTMNTPVEKLTDYCKKLKEVQMHSFPLQFTLHCALLANKTDLAKALMKAVKEEGFPIRPHYFWPLLVGRRKEKNVQGIIEILKGMQELGVHPDQETYTDYVIPCFDSVNSARAILQENGCLSDSDMFSQAGLRSEAANGNLDFVLSFLKSNTLPISLQSIRSSLLLGFRRSMNINLWSEITELLYKDGRYCQEPRGPTEAVGYFLYNLIDSMSDSEVQAKEEHLRQYFHQLEKMNVKIPENIYRGIRNLLESYHVPELIKDAHLLVESKNLDFQKTVQLTSSELESTLETLKAENQPIRDVLKQLILVLCSEENMQKALELKAKYESDMVTGGYAALINLCCRHDKVEDALNLKEEFDRLDSSAVLDTGKYVGLVRVLAKHGKLQDAINILKEMKEKDVLIKDTTALSFFHMLNGAALRGEIETVKQLHEAIVTLGLAEPSTNISFPLVTVHLEKGDLSTALEVAIDCYEKYKVLPRIHDVLCKLVEKGETDLIQKAMDFVSQEQGEMVMLYDLFFAFLQTGNYKEAKKIIETPGIRARSARLQWFCDRCVANNQVETLEKLVELTQKLFECDRDQMYYNLLKLYKINGDWQRADAVWNKIQEENVIPREKTLRLLAEILREGNQEVPFDVPELWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P42704 As Substrate

P42704

Site	PTM Type	Source
S23	Phosphorylation	Uniprot
S54	Phosphorylation	Uniprot
S75	Phosphorylation	Uniprot
S80	Phosphorylation	Uniprot
K107	Acetylation	Uniprot
K107	Ubiquitination	Uniprot
R128	Methylation	Uniprot
C130	S-Nitrosylation	Uniprot
K138	Acetylation	Uniprot
K155	Acetylation	Uniprot
K155	Ubiquitination	Uniprot
Y160	Phosphorylation	Uniprot
Y165	Phosphorylation	Uniprot
S180	Phosphorylation	Uniprot
K187	Acetylation	Uniprot
R197	Methylation	Uniprot
Y207	Phosphorylation	Uniprot
S236	Phosphorylation	Uniprot
T269	Phosphorylation	Uniprot
Y270	Phosphorylation	Uniprot
Y277	Phosphorylation	Uniprot
K280	Ubiquitination	Uniprot
K287	Ubiquitination	Uniprot
K292	Acetylation	Uniprot
Y320	Phosphorylation	Uniprot
Y334	Phosphorylation	Uniprot
K396	Acetylation	Uniprot
K453	Ubiquitination	Uniprot
S536	Phosphorylation	Uniprot
S543	Phosphorylation	Uniprot
S593	Phosphorylation	Uniprot
R605	Methylation	Uniprot
Y607	Phosphorylation	Uniprot
K613	Acetylation	Uniprot
K613	Ubiquitination	Uniprot
K617	Sumoylation	Uniprot
K617	Ubiquitination	Uniprot
Y623	Phosphorylation	Uniprot
Y633	Phosphorylation	Uniprot
K649	Acetylation	Uniprot
K649	Ubiquitination	Uniprot
K655	Ubiquitination	Uniprot
T660	Phosphorylation	Uniprot
S661	Phosphorylation	Uniprot
S662	Phosphorylation	Uniprot
S666	Phosphorylation	Uniprot
K726	Acetylation	Uniprot
R739	Methylation	Uniprot
S742	Phosphorylation	Uniprot
K750	Acetylation	Uniprot
K777	Acetylation	Uniprot
K851	Acetylation	Uniprot
K864	Acetylation	Uniprot
K868	Acetylation	Uniprot
S882	Phosphorylation	Uniprot
N903	Phosphorylation	Uniprot
T913	Phosphorylation	Uniprot
S920	Phosphorylation	Uniprot
R929	Methylation	Uniprot
C930	S-Nitrosylation	Uniprot
Y958	Phosphorylation	Uniprot
K980	Ubiquitination	Uniprot
Y1016	Phosphorylation	Uniprot
S1022	Phosphorylation	Uniprot
S1025	Phosphorylation	Uniprot
S1026	Phosphorylation	Uniprot
S1029	Phosphorylation	Uniprot
T1031	Phosphorylation	Uniprot
Y1052	Phosphorylation	Uniprot
Y1070	Phosphorylation	Uniprot
Y1082	Phosphorylation	Uniprot
T1084	Phosphorylation	Uniprot
K1098	Ubiquitination	Uniprot
T1136	Phosphorylation	Uniprot
S1138	Phosphorylation	Uniprot
K1163	Ubiquitination	Uniprot
S1171	Phosphorylation	Uniprot
S1175	Phosphorylation	Uniprot
Y1215	Phosphorylation	Uniprot
Y1220	Phosphorylation	Uniprot
K1224	Acetylation	Uniprot
Y1251	Phosphorylation	Uniprot
C1277	S-Nitrosylation	Uniprot
S1306	Phosphorylation	Uniprot
K1317	Acetylation	Uniprot
Y1321	Phosphorylation	Uniprot
S1323	Phosphorylation	Uniprot
S1327	Phosphorylation	Uniprot
K1332	Acetylation	Uniprot
Y1341	Phosphorylation	Uniprot
T1345	Phosphorylation	Uniprot
K1350	Ubiquitination	Uniprot
Y1382	Phosphorylation	Uniprot

Research Backgrounds

P42704_LPPRC_HUMAN

Function:

May play a role in RNA metabolism in both nuclei and mitochondria. In the nucleus binds to HNRPA1-associated poly(A) mRNAs and is part of nmRNP complexes at late stages of mRNA maturation which are possibly associated with nuclear mRNA export. May bind mature mRNA in the nucleus outer membrane. In mitochondria binds to poly(A) mRNA. Plays a role in translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. May be involved in transcription regulation. Cooperates with PPARGC1A to regulate certain mitochondrially encoded genes and gluconeogenic genes and may regulate docking of PPARGC1A to transcription factors. Seems to be involved in the transcription regulation of the multidrug-related genes MDR1 and MVP. Part of a nuclear factor that binds to the invMED1 element of MDR1 and MVP gene promoters. Binds single-stranded DNA (By similarity).

Subcellular Location:

Mitochondrion. Nucleus>Nucleoplasm. Nucleus inner membrane. Nucleus outer membrane.
Note: Seems to be predominantly mitochondrial.

Tissue Specificity:

Subunit Structure:

Interacts with CECR2, HEBP2, MAP1S and UXT. Interacts with PPARGC1A. Interacts with FOXO1 (By similarity). Component of mRNP complexes associated with HNRPA1.

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For Research Use Only.
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LRPPRC Antibody - #DF12421