KIDINS220 Antibody - #DF12415

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Product: KIDINS220 Antibody
Catalog: DF12415
Description: Rabbit polyclonal antibody to KIDINS220
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 186-197 kDa; 197kD(Calculated).
Uniprot: Q9ULH0
RRID: AB_2845220

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
KIDINS220 Antibody detects endogenous levels of total KIDINS220.
RRID:
AB_2845220
Cite Format: Affinity Biosciences Cat# DF12415, RRID:AB_2845220.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Ankyrin repeat-rich membrane-spanning protein; arms; KDIS_HUMAN; kidins220; Kinase D interacting substance of 220 kDa; Kinase D-interacting substrate of 220 kDa; rgd 619949;

Immunogens

Immunogen:
Uniprot:

Q9ULH0(KDIS_HUMAN) >>Visit HPA database.

Gene(ID):
KIDINS220(57498)
Expression:
Q9ULH0 KDIS_HUMAN:

Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.

Sequence:
MSVLISQSVINYVEEENIPALKALLEKCKDVDERNECGQTPLMIAAEQGNLEIVKELIKNGANCNLEDLDNWTALISASKEGHVHIVEELLKCGVNLEHRDMGGWTALMWACYKGRTDVVELLLSHGANPSVTGLYSVYPIIWAAGRGHADIVHLLLQNGAKVNCSDKYGTTPLVWAARKGHLECVKHLLAMGADVDQEGANSMTALIVAVKGGYTQSVKEILKRNPNVNLTDKDGNTALMIASKEGHTEIVQDLLDAGTYVNIPDRSGDTVLIGAVRGGHVEIVRALLQKYADIDIRGQDNKTALYWAVEKGNATMVRDILQCNPDTEICTKDGETPLIKATKMRNIEVVELLLDKGAKVSAVDKKGDTPLHIAIRGRSRKLAELLLRNPKDGRLLYRPNKAGETPYNIDCSHQKSILTQIFGARHLSPTETDGDMLGYDLYSSALADILSEPTMQPPICVGLYAQWGSGKSFLLKKLEDEMKTFAGQQIEPLFQFSWLIVFLTLLLCGGLGLLFAFTVHPNLGIAVSLSFLALLYIFFIVIYFGGRREGESWNWAWVLSTRLARHIGYLELLLKLMFVNPPELPEQTTKALPVRFLFTDYNRLSSVGGETSLAEMIATLSDACEREFGFLATRLFRVFKTEDTQGKKKWKKTCCLPSFVIFLFIIGCIISGITLLAIFRVDPKHLTVNAVLISIASVVGLAFVLNCRTWWQVLDSLLNSQRKRLHNAASKLHKLKSEGFMKVLKCEVELMARMAKTIDSFTQNQTRLVVIIDGLDACEQDKVLQMLDTVRVLFSKGPFIAIFASDPHIIIKAINQNLNSVLRDSNINGHDYMRNIVHLPVFLNSRGLSNARKFLVTSATNGDVPCSDTTGIQEDADRRVSQNSLGEMTKLGSKTALNRRDTYRRRQMQRTITRQMSFDLTKLLVTEDWFSDISPQTMRRLLNIVSVTGRLLRANQISFNWDRLASWINLTEQWPYRTSWLILYLEETEGIPDQMTLKTIYERISKNIPTTKDVEPLLEIDGDIRNFEVFLSSRTPVLVARDVKVFLPCTVNLDPKLREIIADVRAAREQISIGGLAYPPLPLHEGPPRAPSGYSQPPSVCSSTSFNGPFAGGVVSPQPHSSYYSGMTGPQHPFYNRPFFAPYLYTPRYYPGGSQHLISRPSVKTSLPRDQNNGLEVIKEDAAEGLSSPTDSSRGSGPAPGPVVLLNSLNVDAVCEKLKQIEGLDQSMLPQYCTTIKKANINGRVLAQCNIDELKKEMNMNFGDWHLFRSTVLEMRNAESHVVPEDPRFLSESSSGPAPHGEPARRASHNELPHTELSSQTPYTLNFSFEELNTLGLDEGAPRHSNLSWQSQTRRTPSLSSLNSQDSSIEISKLTDKVQAEYRDAYREYIAQMSQLEGGPGSTTISGRSSPHSTYYMGQSSSGGSIHSNLEQEKGKDSEPKPDDGRKSFLMKRGDVIDYSSSGVSTNDASPLDPITEEDEKSDQSGSKLLPGKKSSERSSLFQTDLKLKGSGLRYQKLPSDEDESGTEESDNTPLLKDDKDRKAEGKVERVPKSPEHSAEPIRTFIKAKEYLSDALLDKKDSSDSGVRSSESSPNHSLHNEVADDSQLEKANLIELEDDSHSGKRGIPHSLSGLQDPIIARMSICSEDKKSPSECSLIASSPEENWPACQKAYNLNRTPSTVTLNNNSAPANRANQNFDEMEGIRETSQVILRPSSSPNPTTIQNENLKSMTHKRSQRSSYTRLSKDPPELHAAASSESTGFGEERESIL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULH0 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
Y12 Phosphorylation
K22 Ubiquitination
K27 Ubiquitination
K59 Ubiquitination
Y169 Phosphorylation
S203 Phosphorylation
Y215 Phosphorylation
T216 Phosphorylation
S244 Phosphorylation
K291 Ubiquitination
K303 Ubiquitination
Y307 Phosphorylation
K341 Ubiquitination
K367 Ubiquitination
Y398 Phosphorylation
T406 Phosphorylation
K416 Ubiquitination
Y465 Phosphorylation
K641 Ubiquitination
K732 Ubiquitination
K737 Ubiquitination
K743 Acetylation
K746 Acetylation
K746 Ubiquitination
Y833 Phosphorylation
K854 Ubiquitination
S882 Phosphorylation
S885 Phosphorylation
K891 Ubiquitination
S894 Phosphorylation
K895 Ubiquitination
T903 Phosphorylation
T912 Phosphorylation
T914 Phosphorylation
S918 Phosphorylation P98161 (PKD1)
T927 Phosphorylation
S932 Phosphorylation
S947 Phosphorylation
T949 Phosphorylation
K1007 Ubiquitination
K1013 Ubiquitination
K1057 Ubiquitination
Y1079 Phosphorylation
Y1096 Phosphorylation
Y1125 Phosphorylation
Y1144 Phosphorylation
Y1146 Phosphorylation
R1149 Methylation
Y1150 Phosphorylation
Y1151 Phosphorylation
S1155 Phosphorylation
S1163 Phosphorylation
K1165 Ubiquitination
S1167 Phosphorylation
S1294 Phosphorylation
S1295 Phosphorylation
S1296 Phosphorylation
S1309 Phosphorylation
Y1324 Phosphorylation
S1329 Phosphorylation
S1349 Phosphorylation
S1352 Phosphorylation
T1357 Phosphorylation
S1359 Phosphorylation
S1361 Phosphorylation
S1362 Phosphorylation
S1365 Phosphorylation
S1373 Phosphorylation
K1378 Ubiquitination
Y1383 Phosphorylation
Y1387 Phosphorylation
Y1390 Phosphorylation
S1410 Phosphorylation
S1411 Phosphorylation
Y1460 Phosphorylation
S1471 Phosphorylation
T1477 Phosphorylation
S1486 Phosphorylation
S1488 Phosphorylation
S1521 Phosphorylation
S1526 Phosphorylation
T1528 Phosphorylation
T1534 Phosphorylation
S1555 Phosphorylation
S1559 Phosphorylation
Y1572 Phosphorylation
S1574 Phosphorylation
K1580 Ubiquitination
S1590 Phosphorylation
S1594 Phosphorylation
S1598 Phosphorylation
S1607 Phosphorylation
S1621 Phosphorylation
S1623 Phosphorylation
K1625 Ubiquitination
S1631 Phosphorylation
S1633 Phosphorylation
S1661 Phosphorylation
S1662 Phosphorylation
Y1674 Phosphorylation
T1679 Phosphorylation
S1681 Phosphorylation
T1682 Phosphorylation
T1684 Phosphorylation
S1689 Phosphorylation
S1709 Phosphorylation
S1716 Phosphorylation
S1718 Phosphorylation
S1731 Phosphorylation
K1735 Acetylation
S1737 Phosphorylation
S1741 Phosphorylation
S1746 Phosphorylation
S1757 Phosphorylation

Research Backgrounds

Function:

Promotes a prolonged MAP-kinase signaling by neurotrophins through activation of a Rap1-dependent mechanism. Provides a docking site for the CRKL-C3G complex, resulting in Rap1-dependent sustained ERK activation. May play an important role in regulating postsynaptic signal transduction through the syntrophin-mediated localization of receptor tyrosine kinases such as EPHA4. In cooperation with SNTA1 can enhance EPHA4-induced JAK/STAT activation. Plays a role in nerve growth factor (NGF)-induced recruitment of RAPGEF2 to late endosomes and neurite outgrowth. May play a role in neurotrophin- and ephrin-mediated neuronal outgrowth and in axon guidance during neural development and in neuronal regeneration (By similarity). Modulates stress-induced apoptosis of melanoma cells via regulation of the MEK/ERK signaling pathway.

PTMs:

Tyrosine phosphorylated by NTRK1, NTRK2, EPHB2 and EPHA4. Phosphorylation at Ser-918 is induced by phorbol ester treatment. Phosphorylation by NTRK2 is induced by brain-derived neurotrophic factor (BDNF) and neurotrophin-4/5. Phosphorylation by NTRK1 is induced by nerve growth factor (NGF) (By similarity).

Subcellular Location:

Membrane>Multi-pass membrane protein. Late endosome.
Note: Localized at late endosome before or after nerve growth factor (NGF) stimulation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Abundant in developing and adult neural tissues as well as neuroendocrine cells and dendritic cells. Overexpressed in melanoma and melanoma cell lines.

Subunit Structure:

Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a nerve growth factor (NGF)-dependent manner. Interacts with RAPGEF2; the interaction is strengthened after NGF stimulation. Isoform 2 interacts (via C-terminal domain) with MAGI2 isoform 1 (via PDZ domain). Interacts with NTRK1, NTRK2, NTRK3, ERKL and NGFR. Can form a ternary complex with NGFR and NTRK1 and this complex is affected by the expression levels of KIDINS220/ARMS. An increase in KIDINS220/ARMS expression leads to a decreased association of NGFR and NTRK1. Interacts (via PDZ-binding motif) with SNTA1 and SNTB2 (via PDZ domains). Interacts with EPHA4 and PRKD1.

Family&Domains:

The transmembrane domain mediates interaction with NTRK1.

Research Fields

· Organismal Systems > Nervous system > Neurotrophin signaling pathway.   (View pathway)

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