GSTM4 Antibody - #DF12409

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Product: GSTM4 Antibody
Catalog: DF12409
Description: Rabbit polyclonal antibody to GSTM4
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Rabbit
Mol.Wt.: 26 kDa; 26kD(Calculated).
Uniprot: Q03013
RRID: AB_2845214

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Rabbit(100%)
Clonality:
Polyclonal
Specificity:
GSTM4 Antibody detects endogenous levels of total GSTM4.
RRID:
AB_2845214
Cite Format: Affinity Biosciences Cat# DF12409, RRID:AB_2845214.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Glutathione S transferase M4; Glutathione S transferase Mu 4; Glutathione S-transferase Mu 4; GST class mu 4; GST class-mu 4; GST-Mu2; GSTM4 4; GSTM4; GSTM4-4; GSTM4_HUMAN; GTM4; GTS Mu2; MGC131945; MGC9247;

Immunogens

Immunogen:
Uniprot:

Q03013(GSTM4_HUMAN) >>Visit HPA database.

Gene(ID):
GSTM4(2948)
Expression:
Q03013 GSTM4_HUMAN:

Expressed in a wide variety of tissues.

Sequence:
MSMTLGYWDIRGLAHAIRLLLEYTDSSYEEKKYTMGDAPDYDRSQWLNEKFKLGLDFPNLPYLIDGAHKITQSNAILCYIARKHNLCGETEEEKIRVDILENQAMDVSNQLARVCYSPDFEKLKPEYLEELPTMMQHFSQFLGKRPWFVGDKITFVDFLAYDVLDLHRIFEPNCLDAFPNLKDFISRFEGLEKISAYMKSSRFLPKPLYTRVAVWGNK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
73
Bovine
60
Pig
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q03013 As Substrate

Site PTM Type Enzyme
Y23 Phosphorylation
S26 Phosphorylation
K31 Ubiquitination
K32 Ubiquitination
R43 Methylation
K50 Ubiquitination
K52 Ubiquitination
Y62 Phosphorylation
T71 Phosphorylation
S73 Phosphorylation
Y79 Phosphorylation
K83 Ubiquitination
K94 Ubiquitination
K182 Ubiquitination
S186 Phosphorylation
K193 Ubiquitination
K199 Acetylation
K199 Ubiquitination
K206 Ubiquitination

Research Backgrounds

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. Active on 1-chloro-2,4-dinitrobenzene.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in a wide variety of tissues.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the GST superfamily. Mu family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). A quantitative proteomic study reveals oxidative stress and synapse-related proteins contributed to TDCIPP exposure induced neurotoxicity. Ecotoxicology and environmental safety, 2024 (PubMed: 38262093) [IF=6.8]

Application: WB    Species: Rat    Sample:

Fig. 5. Dysregulated proteins induced by TDCIPP exposure were analyzed by heatmap and Western blot. (A) The heatmap is clustered in rows and the values are protein expression abundance, with blue representing down-regulated proteins and red representing up-regulated proteins. Western blot analysis and quantification of PKN1 (B and F), GSTM4 (C and G), BMAL1 (D and H), NQO1 (E and I) expression levels. Data was shown Mean ± SEM. * , p 

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