GSTM3 Antibody - #DF12408

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Product: GSTM3 Antibody
Catalog: DF12408
Description: Rabbit polyclonal antibody to GSTM3
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 27-29 kDa; 27kD(Calculated).
Uniprot: P21266
RRID: AB_2845213

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(90%), Horse(90%), Sheep(90%), Rabbit(80%), Dog(90%)
Clonality:
Polyclonal
Specificity:
GSTM3 Antibody detects endogenous levels of total GSTM3.
RRID:
AB_2845213
Cite Format: Affinity Biosciences Cat# DF12408, RRID:AB_2845213.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

brain GST; brain type mu glutathione S transferase; glutathione S alkyltransferase M3; glutathione S aryltransferase M3; glutathione S transferase M3 (brain); glutathione S transferase M3; Glutathione S transferase Mu 3; glutathione S transferase, Mu 3; Glutathione S-transferase Mu 3; GST class mu 3; GST class-mu 3; GST5; GSTB; Gstm3; GSTM3-3; GSTM3_HUMAN; GTM3; hGSTM3-3; MGC3310; MGC3704; S (hydroxyalkyl)glutathione lyase M3;

Immunogens

Immunogen:
Uniprot:

P21266(GSTM3_HUMAN) >>Visit HPA database.

Gene(ID):
GSTM3(2947)
Expression:
P21266 GSTM3_HUMAN:

Testis and brain.

Sequence:
MSCESSMVLGYWDIRGLAHAIRLLLEFTDTSYEEKRYTCGEAPDYDRSQWLDVKFKLDLDFPNLPYLLDGKNKITQSNAILRYIARKHNMCGETEEEKIRVDIIENQVMDFRTQLIRLCYSSDHEKLKPQYLEELPGQLKQFSMFLGKFSWFAGEKLTFVDFLTYDILDQNRIFDPKCLDEFPNLKAFMCRFEALEKIAAYLQSDQFCKMPINNKMAQWGNKPVC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
90
Bovine
90
Sheep
90
Dog
90
Rabbit
80
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P21266 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
T28 Phosphorylation
T30 Phosphorylation
S31 Phosphorylation
K35 Ubiquitination
C39 S-Nitrosylation
R47 Methylation
K54 Sumoylation
K54 Ubiquitination
K56 Ubiquitination
Y66 Phosphorylation
K71 Acetylation
K71 Ubiquitination
K73 Ubiquitination
T75 Phosphorylation
R82 Methylation
K87 Ubiquitination
S122 Phosphorylation
K128 Sumoylation
K128 Ubiquitination
K140 Ubiquitination
S143 Phosphorylation
K177 Ubiquitination
K186 Ubiquitination
K197 Ubiquitination
S204 Phosphorylation
C208 S-Nitrosylation
K209 Ubiquitination
K215 Ubiquitination
K222 Ubiquitination

Research Backgrounds

Function:

Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. May govern uptake and detoxification of both endogenous compounds and xenobiotics at the testis and brain blood barriers.

PTMs:

The N-terminus is blocked.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Testis and brain.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the GST superfamily. Mu family.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > Platinum drug resistance.

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Human Diseases > Cancers: Specific types > Hepatocellular carcinoma.   (View pathway)

· Metabolism > Metabolism of other amino acids > Glutathione metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Metabolism of xenobiotics by cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - cytochrome P450.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

References

1). Cardioprotective role of A-cycloglycosylated derivative of Rubiadin in diabetic cardiomyopathy in rats. International Immunopharmacology (PubMed: 36989899) [IF=5.6]
2). Propofol inhibits proliferation and migration of glioma cells by up-regulating lncRNA GAS5. TOXICOLOGY IN VITRO (PubMed: 35085768) [IF=3.2]

Application: WB    Species: human    Sample: U251 cells

Fig. 5. |Propofol regulates the expression of c-Myc, P21 and GSTM3. (A) After transfected by siRNAs and treated with 8 μg/ml propofol for 6 h, Western blot analysis was conducted to measure effects of propofol on protein expression of c-Myc, P21 and GSTM3 in U251 cells.

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