Product: FXR1 Antibody
Catalog: DF12402
Description: Rabbit polyclonal antibody to FXR1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 60-80 kDa; 70kD(Calculated).
Uniprot: P51114
RRID: AB_2845207

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FXR1 Antibody detects endogenous levels of total FXR1.
RRID:
AB_2845207
Cite Format: Affinity Biosciences Cat# DF12402, RRID:AB_2845207.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Fragile X mental retardation autosomal homolog 1; Fragile X mental retardation syndrome related protein 1; Fragile X mental retardation syndrome-related protein 1; FXR1; FXR1_HUMAN; FXR1P; hFXR1p;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P51114 FXR1_HUMAN:

Expressed in all tissues examined including heart, brain, kidney and testis.

Sequence:
MAELTVEVRGSNGAFYKGFIKDVHEDSLTVVFENNWQPERQVPFNEVRLPPPPDIKKEISEGDEVEVYSRANDQEPCGWWLAKVRMMKGEFYVIEYAACDATYNEIVTFERLRPVNQNKTVKKNTFFKCTVDVPEDLREACANENAHKDFKKAVGACRIFYHPETTQLMILSASEATVKRVNILSDMHLRSIRTKLMLMSRNEEATKHLECTKQLAAAFHEEFVVREDLMGLAIGTHGSNIQQARKVPGVTAIELDEDTGTFRIYGESADAVKKARGFLEFVEDFIQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRIEGDNENKLPREDGMVPFVFVGTKESIGNVQVLLEYHIAYLKEVEQLRMERLQIDEQLRQIGSRSYSGRGRGRRGPNYTSGYGTNSELSNPSETESERKDELSDWSLAGEDDRDSRHQRDSRRRPGGRGRSVSGGRGRGGPRGGKSSISSVLKDPDSNPYSLLDNTESDQTADTDASESHHSTNRRRRSRRRRTDEDAVLMDGMTESDTASVNENGLVTVADYISRAESQSRQRNLPRETLAKNKKEMAKDVIEEHGPSEKAINGPTSASGDDISKLQRTPGEEKINTLKEENTQEAAVLNGVS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Chicken
92
Zebrafish
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P51114 As Substrate

Site PTM Type Enzyme
A2 Acetylation
S11 Phosphorylation
K17 Ubiquitination
K56 Ubiquitination
K57 Ubiquitination
S60 Phosphorylation
Y68 Phosphorylation
K83 Ubiquitination
Y103 Phosphorylation
K119 Ubiquitination
K128 Ubiquitination
K148 Ubiquitination
K179 Ubiquitination
R190 Methylation
S191 Phosphorylation
K195 Ubiquitination
K207 Ubiquitination
K213 Ubiquitination
K246 Ubiquitination
Y265 Phosphorylation
S268 Phosphorylation
K273 Ubiquitination
K295 Ubiquitination
K302 Ubiquitination
K310 Ubiquitination
K325 Ubiquitination
Y353 Phosphorylation
Y357 Phosphorylation
R365 Methylation
R368 Methylation
R376 Methylation
R386 Methylation
T401 Phosphorylation
S406 Phosphorylation
S409 Phosphorylation
T411 Phosphorylation
S413 Phosphorylation
K416 Ubiquitination
S420 Phosphorylation Q13153 (PAK1)
S423 Phosphorylation
S432 Phosphorylation
S438 Phosphorylation
R445 Methylation
R453 Methylation
R455 Methylation
R459 Methylation
K462 Methylation
S464 Phosphorylation
S467 Phosphorylation
K470 Ubiquitination
Y477 Phosphorylation
S478 Phosphorylation
T483 Phosphorylation
S485 Phosphorylation
T488 Phosphorylation
T491 Phosphorylation
S494 Phosphorylation
S496 Phosphorylation
S499 Phosphorylation
T500 Phosphorylation
S506 Phosphorylation
T511 Phosphorylation
Y540 Phosphorylation
S546 Phosphorylation
S576 Phosphorylation
S585 Phosphorylation
S587 Phosphorylation
S592 Phosphorylation
T597 Phosphorylation
T605 Phosphorylation
T611 Phosphorylation

Research Backgrounds

Function:

RNA-binding protein required for embryonic and postnatal development of muscle tissue. May regulate intracellular transport and local translation of certain mRNAs (By similarity).

PTMs:

Arg-445 is dimethylated, probably to asymmetric dimethylarginine.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in all tissues examined including heart, brain, kidney and testis.

Subunit Structure:

Interacts with FMR1. Interacts with FRX2. Interacts with TDRD3. Interacts with HABP4. Interacts with CYFIP2 but not with CYFIP1 (By similarity).

(Microbial infection) Interacts with Sindbis virus non-structural protein 3 (via C-terminus); this interaction inhibits the formation of host stress granules on viral mRNAs and the nsp3-FXR1 complexes bind viral RNAs and probably orchestrate the assembly of viral replication complexes.

Family&Domains:

The tandem Agenet-like domains preferentially recognize trimethylated histone peptides.

Belongs to the FMR1 family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

References

1). Quercetin improves nonalcoholic fatty liver by ameliorating inflammation, oxidative stress, and lipid metabolism in db/db mice. Phytotherapy Research, 2019 (PubMed: 31452288) [IF=7.2]

Application: WB    Species: mouse    Sample: liver

FIGURE 5 |Effects of quercetin on Bile acid and farnesoid X receptor 1 (FXR1)/Takeda G‐protein‐coupled receptor 5 (TGR5) axis of db/db mice.(a) Levels of total bile acids (TBAs) in serum and liver in different groups. (b) The relative protein levels of FXR1 and TGR5 in liver of mice normalized to β‐actin as a loading control. Each bar represents the mean ± SEM for groups of six. *p < .05, **p < .01, compared with db/m mice as indicated; #p < .05, ##p < .01, compared with db/db mice as indicated

2). Resveratrol ameliorates triglyceride accumulation through FXR deacetylation in high glucose-treated HepG2 cells. Journal of Functional Foods, 2023 [IF=5.6]

Application: IF/ICC    Species: Mouse    Sample: 4T1 cells

Figure 4. Cellular uptake of Cy5-labeled NPs in 4T1 cells and fluorescence distribution of Cy5-labeled NPs in 4T1 tumor spheroids. (A) Cellular uptake of Cy5-labeled NPs in 4T1 cells after 2 or 4 h treatment. The CLSM images of 4T1 cells incubated with Cy5 (1 μg/mL, red), Cy5/Oxi-αCD NPs or Cy5/FA-Oxi-αCD NPs (containing 1 μg/mL of Cy5) at 37 °C for 2 or 4 h. Cell nuclei were stained with DAPI (blue). Scale bar represents 20 μm. (B) The semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of intracellular NPs (red) is in Figure 4(A). (C) Cellular uptake of Cy5-labeled NPs in 4T1 cells with or without FR antibody treatment. (D) The semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of intracellular NPs (red) in Figure 4(C). (E) Fluorescence distribution of Cy5-labeled NPs (red) in 4T1 tumor spheroids. Tumor spheroid sections were observed at given time points by CLSM. Cell nuclei were stained with DAPI (blue). Scale bar represents 20 μm for 2 h and 50 μm for 4 h. (F) Semi-quantitative analysis of the corresponding Cy5 fluorescence intensity of NPs (red) in tumor spheroid sections. *, statistically different at p 

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