ERM / Etv5 Antibody | Affinity Biosciences

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Western blot analysis of extracts from B16F10, using ETV5 antibody.

Product:	ERM / Etv5 Antibody
Catalog:	DF12396
Description:	Rabbit polyclonal antibody to ERM / Etv5
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.:	58 kDa; 58kD(Calculated).
Uniprot:	P41161
RRID:	AB_2845201

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(89%)

Clonality:

Polyclonal

Specificity:

ERM / Etv5 Antibody detects endogenous levels of total ERM / Etv5.

RRID:

AB_2845201
Cite Format: Affinity Biosciences Cat# DF12396, RRID:AB_2845201.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ERM; Ets related protein ERM; ETS translocation variant 5; Ets variant gene 5; Ets-related protein ERM; ETV5; ETV5_HUMAN;

Immunogens

Immunogen:

Uniprot:

P41161(ETV5_HUMAN) >>Visit HPA database.

Gene(ID):

ETV5(2119)

Expression:

P41161 ETV5_HUMAN:

Ubiquitous.

Sequence:

P41161 ETV5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDGFYDQQVPFMVPGKSRSEECRGRPVIDRKRKFLDTDLAHDSEELFQDLSQLQEAWLAEAQVPDDEQFVPDFQSDNLVLHAPPPTKIKRELHSPSSELSSCSHEQALGANYGEKCLYNYCAYDRKPPSGFKPLTPPTTPLSPTHQNPLFPPPQATLPTSGHAPAAGPVQGVGPAPAPHSLPEPGPQQQTFAVPRPPHQPLQMPKMMPENQYPSEQRFQRQLSEPCHPFPPQPGVPGDNRPSYHRQMSEPIVPAAPPPPQGFKQEYHDPLYEHGVPGMPGPPAHGFQSPMGIKQEPRDYCVDSEVPNCQSSYMRGGYFSSSHEGFSYEKDPRLYFDDTCVVPERLEGKVKQEPTMYREGPPYQRRGSLQLWQFLVTLLDDPANAHFIAWTGRGMEFKLIEPEEVARRWGIQKNRPAMNYDKLSRSLRYYYEKGIMQKVAGERYVYKFVCDPDALFSMAFPDNQRPFLKAESECHLSEEDTLPLTHFEDSPAYLLDMDRCSSLPYAEGFAY

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Rabbit

100

Dog

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P41161 As Substrate

P41161

Site	PTM Type	Enzyme	Source
Y5	Phosphorylation		Uniprot
K89	Sumoylation		Uniprot
S94	Phosphorylation		Uniprot
S248	Phosphorylation		Uniprot
K263	Sumoylation		Uniprot
K293	Sumoylation		Uniprot
S310	Phosphorylation		Uniprot
K329	Ubiquitination		Uniprot
K350	Sumoylation		Uniprot
S367	Phosphorylation	P17612 (PRKACA)	Uniprot
Y443	Phosphorylation		Uniprot
K468	Sumoylation		Uniprot

Research Backgrounds

P41161_ETV5_HUMAN

Function:

Binds to DNA sequences containing the consensus nucleotide core sequence 5'-GGAA.-3'.

Subcellular Location:

Nucleus.

Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts (via C-terminal) with ZMYM5 (via N-terminal 120 amino acid region).

Family&Domains:

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

· Human Diseases > Cancers: Specific types > Prostate cancer. (View pathway)

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ERM / Etv5 Antibody - #DF12396