Product: EIF2B1 Antibody
Catalog: DF12392
Description: Rabbit polyclonal antibody to EIF2B1
Application: WB IHC
Reactivity: Human
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: Q14232
RRID: AB_2845197

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(92%), Chicken(92%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
EIF2B1 Antibody detects endogenous levels of total EIF2B1.
RRID:
AB_2845197
Cite Format: Affinity Biosciences Cat# DF12392, RRID:AB_2845197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EI2BA_HUMAN; eIF-2B GDP-GTP exchange factor subunit alpha; EIF2B; Eif2b1; EIF2BA; Eukaryotic translation initiation factor 2B subunit 1 alpha 26kDa; Eukaryotic translation initiation factor 2B subunit alpha; Translation initiation factor eIF-2B subunit alpha;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MDDKELIEYFKSQMKEDPDMASAVAAIRTLLEFLKRDKGETIQGLRANLTSAIETLCGVDSSVAVSSGGELFLRFISLASLEYSDYSKCKKIMIERGELFLRRISLSRNKIADLCHTFIKDGATILTHAYSRVVLRVLEAAVAAKKRFSVYVTESQPDLSGKKMAKALCHLNVPVTVVLDAAVGYIMEKADLVIVGAEGVVENGGIINKIGTNQMAVCAKAQNKPFYVVAESFKFVRLFPLNQQDVPDKFKYKADTLKVAQTGQDLKEEHPWVDYTAPSLITLLFTDLGVLTPSAVSDELIKLYL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Xenopus
100
Zebrafish
100
Rabbit
100
Dog
92
Chicken
92
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14232 As Substrate

Site PTM Type Enzyme
K11 Ubiquitination
K15 Ubiquitination
T29 Phosphorylation
K35 Acetylation
K35 Ubiquitination
K38 Ubiquitination
Y83 Phosphorylation
S84 Phosphorylation
S105 Phosphorylation
S107 Phosphorylation
Y130 Phosphorylation
K145 Methylation
K145 Ubiquitination
S149 Phosphorylation
K163 Acetylation
K253 Ubiquitination
K258 Ubiquitination

Research Backgrounds

Function:

Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.

Subunit Structure:

Complex of five different subunits; alpha, beta, gamma, delta and epsilon.

Family&Domains:

Belongs to the eIF-2B alpha/beta/delta subunits family.

Research Fields

· Genetic Information Processing > Translation > RNA transport.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.