DYNC2LI1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from human kidney tissue, using DYNC2LI1 Antibody.

DF12389 at 1/100 staining Mouse testis tissue by IHC-P.

Product:	DYNC2LI1 Antibody
Catalog:	DF12389
Description:	Rabbit polyclonal antibody to DYNC2LI1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	40 kDa; 40kD(Calculated).
Uniprot:	Q8TCX1
RRID:	AB_2845194

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(92%), Zebrafish(92%), Bovine(92%), Horse(92%), Sheep(92%), Rabbit(100%), Dog(92%), Chicken(92%)

Clonality:

Polyclonal

Specificity:

DYNC2LI1 Antibody detects endogenous levels of total DYNC2LI1.

RRID:

AB_2845194
Cite Format: Affinity Biosciences Cat# DF12389, RRID:AB_2845194.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CGI-60; Cytoplasmic dynein 2 light intermediate chain 1; D2LIC; DC2L1_HUMAN; DYNC2LI1; Dynein 2 light intermediate chain; Dynein, cytoplasmic 2, light intermediate chain 1; LIC3;

Immunogens

Immunogen:

Uniprot:

Q8TCX1(DC2L1_HUMAN) >>Visit HPA database.

Gene(ID):

DYNC2LI1(51626)

Expression:

Q8TCX1 DC2L1_HUMAN:

Expressed in bone, brain, kidney, and cartilage (PubMed:26077881, PubMed:26130459). Lower expression in heart, liver, lung, placenta and thymus (PubMed:26077881).

Sequence:

Q8TCX1 DC2L1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MPSETLWEIAKAEVEKRGINGSEGDGAEIAEKFVFFIGSKNGGKTTIILRCLDRDEPPKPTLALEYTYGRRAKGHNTPKDIAHFWELGGGTSLLDLISIPITGDTLRTFSLVLVLDLSKPNDLWPTMENLLQATKSHVDKVIMKLGKTNAKAVSEMRQKIWNNMPKDHPDHELIDPFPVPLVIIGSKYDVFQDFESEKRKVICKTLRFVAHYYGASLMFTSKSEALLLKIRGVINQLAFGIDKSKSICVDQNKPLFITAGLDSFGQIGSPPVPENDIGKLHAHSPMELWKKVYEKLFPPKSINTLKDIKDPARDPQYAENEVDEMRIQKDLELEQYKRSSSKSWKQIELDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Pig

Horse

Bovine

Sheep

Dog

Zebrafish

Chicken

Xenopus

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8TCX1 As Substrate

Q8TCX1

Site	PTM Type	Source
K11	Ubiquitination	Uniprot
K151	Ubiquitination	Uniprot
S154	Phosphorylation	Uniprot
K198	Ubiquitination	Uniprot
T220	Phosphorylation	Uniprot
S221	Phosphorylation	Uniprot
K329	Ubiquitination	Uniprot
K337	Ubiquitination	Uniprot

Research Backgrounds

Q8TCX1_DC2L1_HUMAN

Function:

Required for correct intraflagellar transport (IFT), the bi-directional movement of particles required for the assembly, maintenance and functioning of primary cilia. Involved in the regulation of ciliary length.

Subcellular Location:

Cytoplasm. Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body. Cytoplasm>Cytoskeleton>Cilium axoneme. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Localizes to the apical cytoplasm.

Tissue Specificity:

Expressed in bone, brain, kidney, and cartilage. Lower expression in heart, liver, lung, placenta and thymus.

Subunit Structure:

The cytoplasmic dynein complex 2 is probably composed by a DYNC2H1 homodimer and a number of DYNC2LI1 light intermediate chains.

Family&Domains:

Belongs to the dynein light intermediate chain family.

Research Fields

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

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DYNC2LI1 Antibody - #DF12389