DIS3 Antibody - #DF12383

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Product: DIS3 Antibody
Catalog: DF12383
Description: Rabbit polyclonal antibody to DIS3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 110 kDa; 109kD(Calculated).
Uniprot: Q9Y2L1
RRID: AB_2845188

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(91%), Xenopus(91%)
Clonality:
Polyclonal
Specificity:
DIS3 Antibody detects endogenous levels of total DIS3.
RRID:
AB_2845188
Cite Format: Affinity Biosciences Cat# DF12383, RRID:AB_2845188.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

2810028N01Rik; bA555G22; Dis3; DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease; DIS3 mitotic control homolog (S. cerevisiae); DIS3 mitotic control homolog; DIS3 mitotic control, S. cerevisiae, homolog of; dis3p; EXOSC11; Exosome complex exonuclease RRP44; exosome component 11; KIAA1008; Mitotic control protein dis3 homolog; Protein DIS3 homolog; Ribosomal RNA processing protein 44; Ribosomal RNA processing protein 44, S. cerevisiae, homolog of; Ribosomal RNA-processing protein 44; RP11-342J4.3; RRP44; RRP44_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9Y2L1(RRP44_HUMAN) >>Visit HPA database.

Gene(ID):
DIS3(22894)
Expression:
Q9Y2L1 RRP44_HUMAN:

Widely expressed.

Sequence:
MLKSKTFLKKTRAGGVMKIVREHYLRDDIGCGAPGCAACGGAHEGPALEPQPQDPASSVCPQPHYLLPDTNVLLHQIDVLEDPAIRNVIVLQTVLQEVRNRSAPVYKRIRDVTNNQEKHFYTFTNEHHRETYVEQEQGENANDRNDRAIRVAAKWYNEHLKKMSADNQLQVIFITNDRRNKEKAIEEGIPAFTCEEYVKSLTANPELIDRLACLSEEGNEIESGKIIFSEHLPLSKLQQGIKSGTYLQGTFRASRENYLEATVWIHGDNEENKEIILQGLKHLNRAVHEDIVAVELLPKSQWVAPSSVVLHDEGQNEEDVEKEEETERMLKTAVSEKMLKPTGRVVGIIKRNWRPYCGMLSKSDIKESRRHLFTPADKRIPRIRIETRQASTLEGRRIIVAIDGWPRNSRYPNGHFVRNLGDVGEKETETEVLLLEHDVPHQPFSQAVLSFLPKMPWSITEKDMKNREDLRHLCICSVDPPGCTDIDDALHCRELENGNLEVGVHIADVSHFIRPGNALDQESARRGTTVYLCEKRIDMVPELLSSNLCSLKCDVDRLAFSCIWEMNHNAEILKTKFTKSVINSKASLTYAEAQLRIDSANMNDDITTSLRGLNKLAKILKKRRIEKGALTLSSPEVRFHMDSETHDPIDLQTKELRETNSMVEEFMLLANISVAKKIHEEFSEHALLRKHPAPPPSNYEILVKAARSRNLEIKTDTAKSLAESLDQAESPTFPYLNTLLRILATRCMMQAVYFCSGMDNDFHHYGLASPIYTHFTSPIRRYADVIVHRLLAVAIGADCTYPELTDKHKLADICKNLNFRHKMAQYAQRASVAFHTQLFFKSKGIVSEEAYILFVRKNAIVVLIPKYGLEGTVFFEEKDKPNPQLIYDDEIPSLKIEDTVFHVFDKVKVKIMLDSSNLQHQKIRMSLVEPQIPGISIPTDTSNMDLNGPKKKKMKLGK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
91
Zebrafish
91
Chicken
91
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y2L1 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K5 Acetylation
R12 Methylation
K18 Acetylation
K18 Ubiquitination
K107 Ubiquitination
T113 Phosphorylation
K118 Ubiquitination
T124 Phosphorylation
K154 Ubiquitination
S164 Phosphorylation
T175 Phosphorylation
R178 Methylation
K183 Ubiquitination
K199 Ubiquitination
R210 Methylation
S215 Phosphorylation
K225 Ubiquitination
K236 Ubiquitination
K242 Ubiquitination
K281 Ubiquitination
K299 Ubiquitination
K331 Methylation
K331 Sumoylation
K331 Ubiquitination
K337 Acetylation
K337 Ubiquitination
K340 Ubiquitination
K362 Ubiquitination
K535 Ubiquitination
K579 Ubiquitination
K585 Ubiquitination
S599 Phosphorylation
T608 Phosphorylation
K627 Ubiquitination
S633 Phosphorylation
S634 Phosphorylation
K654 Ubiquitination
T659 Phosphorylation
S673 Phosphorylation
K677 Ubiquitination
K690 Ubiquitination
K704 Ubiquitination
K714 Ubiquitination
K719 Ubiquitination
S730 Phosphorylation
K809 Ubiquitination
K815 Acetylation
K815 Ubiquitination
K880 Ubiquitination
K895 Ubiquitination
K906 Ubiquitination
K910 Ubiquitination
K922 Acetylation
K922 Ubiquitination

Research Backgrounds

Function:

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus. Nucleus>Nucleoplasm. Nucleus.
Note: Predominantly located in the nucleus (PubMed:20531386). According to PubMed:12429849, found in the nucleolus (PubMed:12429849). According to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3 (PubMed:20531386).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Component of the RNA exosome complex. The catalytically inactive RNA exosome core (Exo-9) complex is believed to associate with catalytic subunits EXOSC10, and DIS3 or DIS3L in cytoplasmic- and nuclear-specific RNA exosome complex forms.

Family&Domains:

Belongs to the RNR ribonuclease family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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