DCTN6 Antibody - #DF12381

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Product: DCTN6 Antibody
Catalog: DF12381
Description: Rabbit polyclonal antibody to DCTN6
Application: WB IHC IF/ICC
Reactivity: Human, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 27 kDa; 21kD(Calculated).
Uniprot: O00399
RRID: AB_2845186

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Rat
Prediction:
Pig(83%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%)
Clonality:
Polyclonal
Specificity:
DCTN6 Antibody detects endogenous levels of total DCTN6.
RRID:
AB_2845186
Cite Format: Affinity Biosciences Cat# DF12381, RRID:AB_2845186.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DCTN6; DCTN6_HUMAN; Dynactin 6; Dynactin subunit 6; Dynactin subunit p27; Novel RGD containing protein; p27; Protein WS-3; Protein WS3; WS 3; WS3;

Immunogens

Immunogen:
Uniprot:

O00399(DCTN6_HUMAN) >>Visit HPA database.

Gene(ID):
DCTN6(10671)
Expression:
O00399 DCTN6_HUMAN:

Ubiquitous.

Sequence:
MAEKTQKSVKIAPGAVVCVESEIRGDVTIGPRTVIHPKARIIAEAGPIVIGEGNLIEEQALIINAYPDNITPDTEDPEPKPMIIGTNNVFEVGCYSQAMKMGDNNVIESKAYVGRNVILTSGCIIGACCNLNTFEVIPENTVIYGADCLRRVQTERPQPQTLQLDFLMKILPNYHHLKKTMKGSSTPVKN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Sheep
92
Dog
92
Rabbit
92
Pig
83
Xenopus
63
Zebrafish
50
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00399 As Substrate

Site PTM Type Enzyme
K110 Ubiquitination
K178 Ubiquitination
T186 Phosphorylation P06493 (CDK1)

Research Backgrounds

PTMs:

Phosphorylation at Thr-186 by CDK1 during mitotic prometaphase creates a binding site for PLK1 that facilitates its recruitment to kinetochores.

Subcellular Location:

Cytoplasm>Cytoskeleton. Chromosome>Centromere>Kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Member of the pointed-end complex of the dynactin shoulder complex which contains DCTN4, DCTN5 and DCTN6 subunits and ACTR10. Within the complex DCTN6 forms a heterodimer with DCTN5. Interacts with PLK1.

Family&Domains:

Belongs to the dynactin subunits 5/6 family. Dynactin subunit 6 subfamily.

Research Fields

· Organismal Systems > Excretory system > Vasopressin-regulated water reabsorption.

Restrictive clause

 

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For Research Use Only.
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