DAAM1 Antibody - #DF12380
| Product: | DAAM1 Antibody |
| Catalog: | DF12380 |
| Description: | Rabbit polyclonal antibody to DAAM1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 123 kDa; 123kD(Calculated). |
| Uniprot: | Q9Y4D1 |
| RRID: | AB_2845185 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12380, RRID:AB_2845185.
Fold/Unfold
DAAM 1; Dishevelled associated activator of morphogenesis 1; FLJ41657; KIAA0666;
Immunogens
- Q9Y4D1 DAAM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAPRKRGGRGISFIFCCFRNNDHPEITYRLRNDSNFALQTMEPALPMPPVEELDVMFSELVDELDLTDKHREAMFALPAEKKWQIYCSKKKDQEENKGATSWPEFYIDQLNSMAARKSLLALEKEEEEERSKTIESLKTALRTKPMRFVTRFIDLDGLSCILNFLKTMDYETSESRIHTSLIGCIKALMNNSQGRAHVLAHSESINVIAQSLSTENIKTKVAVLEILGAVCLVPGGHKKVLQAMLHYQKYASERTRFQTLINDLDKSTGRYRDEVSLKTAIMSFINAVLSQGAGVESLDFRLHLRYEFLMLGIQPVIDKLREHENSTLDRHLDFFEMLRNEDELEFAKRFELVHIDTKSATQMFELTRKRLTHSEAYPHFMSILHHCLQMPYKRSGNTVQYWLLLDRIIQQIVIQNDKGQDPDSTPLENFNIKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKTQEKEEMMQTLNKMKEKLEKETTEHKQVKQQVADLTAQLHELSRRAVCASIPGGPSPGAPGGPFPSSVPGSLLPPPPPPPLPGGMLPPPPPPLPPGGPPPPPGPPPLGAIMPPPGAPMGLALKKKSIPQPTNALKSFNWSKLPENKLEGTVWTEIDDTKVFKILDLEDLERTFSAYQRQQDFFVNSNSKQKEADAIDDTLSSKLKVKELSVIDGRRAQNCNILLSRLKLSNDEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDIDLLEEHKHELDRMAKADRFLFEMSRINHYQQRLQSLYFKKKFAERVAEVKPKVEAIRSGSEEVFRSGALKQLLEVVLAFGNYMNKGQRGNAYGFKISSLNKIADTKSSIDKNITLLHYLITIVENKYPSVLNLNEELRDIPQAAKVNMTELDKEISTLRSGLKAVETELEYQKSQPPQPGDKFVSVVSQFITVASFSFSDVEDLLAEAKDLFTKAVKHFGEEAGKIQPDEFFGIFDQFLQAVSEAKQENENMRKKKEEEERRARMEAQLKEQRERERKMRKAKENSEESGEFDDLVSALRSGEVFDKDLSKLKRNRKRITNQMTDSSRERPITKLNF
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y4D1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| R9 | Methylation | Uniprot | |
| S12 | Phosphorylation | Uniprot | |
| T27 | Phosphorylation | Uniprot | |
| Y28 | Phosphorylation | Uniprot | |
| S34 | Phosphorylation | Uniprot | |
| K97 | Ubiquitination | Uniprot | |
| S118 | Phosphorylation | Uniprot | |
| K124 | Ubiquitination | Uniprot | |
| K132 | Ubiquitination | Uniprot | |
| K186 | Acetylation | Uniprot | |
| Y247 | Phosphorylation | Uniprot | |
| T255 | Phosphorylation | Uniprot | |
| T259 | Phosphorylation | Uniprot | |
| Y271 | Phosphorylation | Uniprot | |
| Y401 | Phosphorylation | Uniprot | |
| T425 | Phosphorylation | Uniprot | |
| K433 | Ubiquitination | Uniprot | |
| K476 | Acetylation | Uniprot | |
| K489 | Acetylation | Uniprot | |
| K491 | Acetylation | Uniprot | |
| T499 | Phosphorylation | Uniprot | |
| K638 | Ubiquitination | Uniprot | |
| Y652 | Phosphorylation | Uniprot | |
| K679 | Ubiquitination | Uniprot | |
| K748 | Ubiquitination | Uniprot | |
| S776 | Phosphorylation | Uniprot | |
| S799 | Phosphorylation | Uniprot | |
| S801 | Phosphorylation | Uniprot | |
| Y833 | Phosphorylation | Uniprot | |
| K842 | Ubiquitination | Uniprot | |
| Y912 | Phosphorylation | Uniprot | |
| S1027 | Phosphorylation | Uniprot | |
| T1061 | Phosphorylation | Uniprot | |
| T1074 | Phosphorylation | Uniprot |
Research Backgrounds
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells (By similarity). Together with DAAM2, required for myocardial maturation and sarcomere assembly (By similarity).
Cytoplasm. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Perinuclear.
Expressed in all tissues examined.
Homodimer. Interacts with CIP4, FNBP1 and FNBP1L. Interacts with the SH3 domains of Abl, BTK, endophilin, spectrin and SRC. Binds specifically to GTP-bound CDC42 and RHOA. Interacts with INTU; INTU mediates the indirect interaction between DAAM1 and NPHP4.
The C-terminal DAD domain may participate in intramolecular interactions with the N-terminus.
The DAD domain regulates activation via by an autoinhibitory interaction with the GBD/FH3 domain. This autoinhibition is released upon competitive binding of an activated GTPase. The release of DAD allows the FH2 domain to then nucleate and elongate nonbranched actin filaments (By similarity).
Belongs to the formin homology family.
Research Fields
· Environmental Information Processing > Signal transduction > Wnt signaling pathway. (View pathway)
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