CPS1 Antibody - #DF12371

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Product: CPS1 Antibody
Catalog: DF12371
Description: Rabbit polyclonal antibody to CPS1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 116 kDa,165 kDa; 165kD(Calculated).
Uniprot: P31327
RRID: AB_2845176

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(89%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
CPS1 Antibody detects endogenous levels of total CPS1.
RRID:
AB_2845176
Cite Format: Affinity Biosciences Cat# DF12371, RRID:AB_2845176.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Carbamoyl phosphate synthase [ammonia]; Carbamoyl phosphate synthase [ammonia] mitochondrial; Carbamoyl phosphate synthase; Carbamoyl phosphate synthetase 1; Carbamoyl phosphate synthetase 1 mitochondrial; Carbamoyl phosphate synthetase I; Carbamoyl-phosphate synthase [ammonia]; Carbamoyl-phosphate synthetase I; Carbamoylphosphate synthase; Carbamoylphosphate synthetase 1; Carbamoylphosphate synthetase I; CPS 1; Cps1; CPSase 1; CPSase I; CPSASE1; CPSM_HUMAN; mitochondrial; MS738;

Immunogens

Immunogen:
Uniprot:

P31327(CPSM_HUMAN) >>Visit HPA database.

Gene(ID):
CPS1(1373)
Expression:
P31327 CPSM_HUMAN:

Primarily in the liver and small intestine.

Sequence:
MTRILTAFKVVRTLKTGFGFTNVTAHQKWKFSRPGIRLLSVKAQTAHIVLEDGTKMKGYSFGHPSSVAGEVVFNTGLGGYPEAITDPAYKGQILTMANPIIGNGGAPDTTALDELGLSKYLESNGIKVSGLLVLDYSKDYNHWLATKSLGQWLQEEKVPAIYGVDTRMLTKIIRDKGTMLGKIEFEGQPVDFVDPNKQNLIAEVSTKDVKVYGKGNPTKVVAVDCGIKNNVIRLLVKRGAEVHLVPWNHDFTKMEYDGILIAGGPGNPALAEPLIQNVRKILESDRKEPLFGISTGNLITGLAAGAKTYKMSMANRGQNQPVLNITNKQAFITAQNHGYALDNTLPAGWKPLFVNVNDQTNEGIMHESKPFFAVQFHPEVTPGPIDTEYLFDSFFSLIKKGKATTITSVLPKPALVASRVEVSKVLILGSGGLSIGQAGEFDYSGSQAVKAMKEENVKTVLMNPNIASVQTNEVGLKQADTVYFLPITPQFVTEVIKAEQPDGLILGMGGQTALNCGVELFKRGVLKEYGVKVLGTSVESIMATEDRQLFSDKLNEINEKIAPSFAVESIEDALKAADTIGYPVMIRSAYALGGLGSGICPNRETLMDLSTKAFAMTNQILVEKSVTGWKEIEYEVVRDADDNCVTVCNMENVDAMGVHTGDSVVVAPAQTLSNAEFQMLRRTSINVVRHLGIVGECNIQFALHPTSMEYCIIEVNARLSRSSALASKATGYPLAFIAAKIALGIPLPEIKNVVSGKTSACFEPSLDYMVTKIPRWDLDRFHGTSSRIGSSMKSVGEVMAIGRTFEESFQKALRMCHPSIEGFTPRLPMNKEWPSNLDLRKELSEPSSTRIYAIAKAIDDNMSLDEIEKLTYIDKWFLYKMRDILNMEKTLKGLNSESMTEETLKRAKEIGFSDKQISKCLGLTEAQTRELRLKKNIHPWVKQIDTLAAEYPSVTNYLYVTYNGQEHDVNFDDHGMMVLGCGPYHIGSSVEFDWCAVSSIRTLRQLGKKTVVVNCNPETVSTDFDECDKLYFEELSLERILDIYHQEACGGCIISVGGQIPNNLAVPLYKNGVKIMGTSPLQIDRAEDRSIFSAVLDELKVAQAPWKAVNTLNEALEFAKSVDYPCLLRPSYVLSGSAMNVVFSEDEMKKFLEEATRVSQEHPVVLTKFVEGAREVEMDAVGKDGRVISHAISEHVEDAGVHSGDATLMLPTQTISQGAIEKVKDATRKIAKAFAISGPFNVQFLVKGNDVLVIECNLRASRSFPFVSKTLGVDFIDVATKVMIGENVDEKHLPTLDHPIIPADYVAIKAPMFSWPRLRDADPILRCEMASTGEVACFGEGIHTAFLKAMLSTGFKIPQKGILIGIQQSFRPRFLGVAEQLHNEGFKLFATEATSDWLNANNVPATPVAWPSQEGQNPSLSSIRKLIRDGSIDLVINLPNNNTKFVHDNYVIRRTAVDSGIPLLTNFQVTKLFAEAVQKSRKVDSKSLFHYRQYSAGKAA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
92
Chicken
89
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P31327 As Substrate

Site PTM Type Enzyme
K15 Ubiquitination
S32 Phosphorylation
T45 Phosphorylation
K55 Acetylation
K119 Acetylation
K119 Ubiquitination
S129 Phosphorylation
S137 Phosphorylation
K138 Acetylation
Y140 Phosphorylation
K147 Ubiquitination
S148 Phosphorylation
K157 Acetylation
K157 Ubiquitination
Y162 Phosphorylation
K171 Ubiquitination
K176 Ubiquitination
K182 Acetylation
K182 Ubiquitination
K197 Acetylation
K197 Ubiquitination
K207 Acetylation
K207 Ubiquitination
K214 Ubiquitination
K219 Acetylation
K219 Ubiquitination
K228 Ubiquitination
K280 Ubiquitination
S284 Phosphorylation
K287 Acetylation
K287 Ubiquitination
S294 Phosphorylation
T295 Phosphorylation
K307 Ubiquitination
T326 Phosphorylation
K328 Ubiquitination
K350 Ubiquitination
S368 Phosphorylation
K412 Acetylation
K412 Ubiquitination
S418 Phosphorylation
S423 Phosphorylation
S430 Phosphorylation
K458 Acetylation
K458 Ubiquitination
S468 Phosphorylation
T488 Phosphorylation
K522 Acetylation
K522 Ubiquitination
K527 Ubiquitination
Y529 Phosphorylation
S537 Phosphorylation
T544 Phosphorylation
R547 Methylation
K553 Acetylation
K553 Ubiquitination
K560 Acetylation
K560 Ubiquitination
S564 Phosphorylation
S569 Phosphorylation
S588 Phosphorylation
Y590 Phosphorylation
C600 S-Nitrosylation
K630 Ubiquitination
Y634 Phosphorylation
S684 Phosphorylation
T706 Phosphorylation
Y732 Phosphorylation
K751 Acetylation
K751 Ubiquitination
K757 Ubiquitination
Y768 Phosphorylation
K772 Methylation
K772 Ubiquitination
K793 Ubiquitination
S794 Phosphorylation
K811 Ubiquitination
S819 Phosphorylation
K831 Acetylation
K831 Ubiquitination
S835 Phosphorylation
K841 Ubiquitination
K856 Acetylation
K856 Ubiquitination
S863 Phosphorylation
K869 Acetylation
K869 Ubiquitination
Y872 Phosphorylation
K875 Acetylation
Y879 Phosphorylation
K889 Ubiquitination
K892 Acetylation
K892 Ubiquitination
S896 Phosphorylation
S898 Phosphorylation
K905 Acetylation
K905 Ubiquitination
K908 Ubiquitination
S913 Phosphorylation
K915 Acetylation
K915 Ubiquitination
S918 Phosphorylation
K919 Acetylation
K919 Ubiquitination
T924 Phosphorylation
K935 Ubiquitination
K1009 Ubiquitination
S1036 Phosphorylation
S1055 Phosphorylation
Y1069 Phosphorylation
K1070 Acetylation
K1074 Ubiquitination
T1078 Phosphorylation
S1079 Phosphorylation
S1090 Phosphorylation
K1100 Ubiquitination
K1107 Acetylation
K1107 Ubiquitination
K1120 Ubiquitination
S1135 Phosphorylation
S1137 Phosphorylation
S1144 Phosphorylation
K1149 Acetylation
K1150 Acetylation
K1150 Ubiquitination
S1159 Phosphorylation
K1168 Ubiquitination
K1183 Acetylation
K1183 Ubiquitination
S1203 Phosphorylation
K1222 Ubiquitination
S1261 Phosphorylation
S1268 Phosphorylation
K1269 Ubiquitination
T1270 Phosphorylation
T1280 Phosphorylation
K1281 Ubiquitination
K1291 Acetylation
K1291 Ubiquitination
T1295 Phosphorylation
Y1305 Phosphorylation
K1309 Ubiquitination
S1314 Phosphorylation
S1331 Phosphorylation
T1332 Phosphorylation
K1356 Acetylation
K1356 Ubiquitination
S1419 Phosphorylation
S1421 Phosphorylation
S1422 Phosphorylation
S1431 Phosphorylation
Y1450 Phosphorylation
T1455 Phosphorylation
K1471 Ubiquitination
K1479 Acetylation
K1479 Ubiquitination
K1486 Acetylation
K1486 Ubiquitination
Y1491 Phosphorylation

Research Backgrounds

Function:

Involved in the urea cycle of ureotelic animals where the enzyme plays an important role in removing excess ammonia from the cell.

PTMs:

Undergoes proteolytic cleavage in the C-terminal region corresponding to the loss of approximately 12 AA residues from the C-terminus.

Succinylated at Lys-287 and Lys-1291. Desuccinylated at Lys-1291 by SIRT5, leading to activation (By similarity).

Glutarylated. Glutarylation levels increase during fasting. Deglutarylated by SIRT5 at Lys-55, Lys-219, Lys-412, Lys-889, Lys-892, Lys-915, Lys-1360 and Lys-1486, leading to activation.

Subcellular Location:

Mitochondrion. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Primarily in the liver and small intestine.

Subunit Structure:

Can form homooligomers (monomers as predominant form and dimers).

Family&Domains:

The type-1 glutamine amidotransferase domain is defective.

Research Fields

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

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