CNOT10 Antibody - #DF12366

(3)  
Product: CNOT10 Antibody
Catalog: DF12366
Description: Rabbit polyclonal antibody to CNOT10
Application: WB IHC IF/ICC
Reactivity: Human, Mouse
Prediction: Horse, Rabbit, Dog, Chicken
Mol.Wt.: 82 kDa; 82kD(Calculated).
Uniprot: Q9H9A5
RRID: AB_2845171

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Horse(100%), Rabbit(83%), Dog(100%), Chicken(91%)
Clonality:
Polyclonal
Specificity:
CNOT10 Antibody detects endogenous levels of total CNOT10.
RRID:
AB_2845171
Cite Format: Affinity Biosciences Cat# DF12366, RRID:AB_2845171.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CCR4 NOT transcription complex subunit 10; CCR4-NOT transcription complex subunit 10; CNOT 10; CNOT10; CNOTA_HUMAN; DKFZp434K115; FLJ12890; FLJ13165;

Immunogens

Immunogen:
Uniprot:

Q9H9A5(CNO10_HUMAN) >>Visit HPA database.

Gene(ID):
CNOT10(25904)
Sequence:
MAADKPADQGAEKHEGTGQSSGITDQEKELSTNAFQAFTSGNYDACLQHLACLQDINKDDYKIILNTAVAEFFKSNQTTTDNLRQTLNQLKNQVHSAVEEMDGLDDVENSMLYYNQAVILYHLRQYTEAISVGEKLYQFIEPFEEKFAQAVCFLLVDLYILTYQAEKALHLLAVLEKMISQGNNNKNGKNETGNNNNKDGSNHKAESGALIEAAKSKIHQYKVRAYIQMKSLKACKREIKSVMNTAGNSAPSLFLKSNFEYLRGNYRKAVKLLNSSNIAEHPGFMKTGECLRCMFWNNLGCIHFAMSKHNLGIFYFKKALQENDNVCAQLSAGSTDPGKKFSGRPMCTLLTNKRYELLYNCGIQLLHIGRPLAAFECLIEAVQVYHANPRLWLRLAECCIAANKGTSEQETKGLPSKKGIVQSIVGQGYHRKIVLASQSIQNTVYNDGQSSAIPVASMEFAAICLRNALLLLPEEQQDPKQENGAKNSNQLGGNTESSESSETCSSKSHDGDKFIPAPPSSPLRKQELENLKCSILACSAYVALALGDNLMALNHADKLLQQPKLSGSLKFLGHLYAAEALISLDRISDAITHLNPENVTDVSLGISSNEQDQGSDKGENEAMESSGKRAPQCYPSSVNSARTVMLFNLGSAYCLRSEYDKARKCLHQAASMIHPKEVPPEAILLAVYLELQNGNTQLALQIIKRNQLLPAVKTHSEVRKKPVFQPVHPIQPIQMPAFTTVQRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Chicken
91
Rabbit
83
Bovine
73
Sheep
73
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9H9A5 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K28 Ubiquitination
K58 Ubiquitination
K74 Ubiquitination
T162 Phosphorylation
K177 Acetylation
K204 Ubiquitination
K215 Ubiquitination
K217 Ubiquitination
K230 Ubiquitination
K240 Ubiquitination
S241 Phosphorylation
T245 Phosphorylation
S249 Phosphorylation
K256 Acetylation
K256 Methylation
K256 Ubiquitination
R263 Methylation
K271 Ubiquitination
K286 Ubiquitination
K318 Ubiquitination
K339 Ubiquitination
K404 Ubiquitination
K412 Ubiquitination
K418 Ubiquitination
K480 Ubiquitination
C504 S-Nitrosylation
K513 Ubiquitination
S521 Phosphorylation
K525 Ubiquitination
K564 Ubiquitination
C633 S-Nitrosylation
K664 Ubiquitination
K713 Ubiquitination

Research Backgrounds

Function:

Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. Is not required for association of CNOT7 to the CCR4-NOT complex.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the CCR4-NOT complex; distinct complexes seem to exist that differ in the participation of probably mutually exclusive catalytic subunits. CNOT10 and CNOT11 form a subcomplex docked to the CNOT1 scaffold.

Family&Domains:

Belongs to the CNOT10 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > RNA degradation.

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