CALCOCO2 Antibody | Affinity Biosciences

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IF/ICC

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CALCOCO2 Antibody - Western blot analysis of extracts from 293, using CALCOCO2 antibody.

CALCOCO2 Antibody - DF12360 staining Hela cells by IF/ICC.

Product:	CALCOCO2 Antibody
Catalog:	DF12360
Description:	Rabbit polyclonal antibody to CALCOCO2
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Mol.Wt.:	52 kDa; 52kD(Calculated).
Uniprot:	Q13137
RRID:	AB_2845165

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Clonality:

Polyclonal

Specificity:

CALCOCO2 Antibody detects endogenous levels of total CALCOCO2.

RRID:

AB_2845165
Cite Format: Affinity Biosciences Cat# DF12360, RRID:AB_2845165.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Antigen nuclear dot 52 kDa protein; CACO2_HUMAN; Calcium binding and coiled coil domain 2; Calcium binding and coiled coil domain containing protein 2; Calcium-binding and coiled-coil domain-containing protein 2; CALCOCO 2; CALCOCO2; MGC17318; NDP52; Nuclear domain 10 protein 52; Nuclear domain 10 protein; Nuclear domain 10 protein NDP52; Nuclear domain protein 52; Nuclear dot protein 52; OTTHUMP00000218344; OTTHUMP00000218345; OTTHUMP00000218346; OTTHUMP00000218347; OTTHUMP00000218349; OTTHUMP00000218351;

Immunogens

Immunogen:

Uniprot:

Q13137(CACO2_HUMAN) >>Visit HPA database.

Gene(ID):

CALCOCO2(10241)

Expression:

Q13137 CACO2_HUMAN:

Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.

Sequence:

Q13137 CACO2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MEETIKDPPTSAVLLDHCHFSQVIFNSVEKFYIPGGDVTCHYTFTQHFIPRRKDWIGIFRVGWKTTREYYTFMWVTLPIDLNNKSAKQQEVQFKAYYLPKDDEYYQFCYVDEDGVVRGASIPFQFRPENEEDILVVTTQGEVEEIEQHNKELCKENQELKDSCISLQKQNSDMQAELQKKQEELETLQSINKKLELKVKEQKDYWETELLQLKEQNQKMSSENEKMGIRVDQLQAQLSTQEKEMEKLVQGDQDKTEQLEQLKKENDHLFLSLTEQRKDQKKLEQTVEQMKQNETTAMKKQQELMDENFDLSKRLSENEIICNALQRQKERLEGENDLLKRENSRLLSYMGLDFNSLPYQVPTSDEGGARQNPGLAYGNPYSGIQESSSPSPLSIKKCPICKADDICDHTLEQQQMQPLCFNCPICDKIFPATEKQIFEDHVFCHSL

PTMs - Q13137 As Substrate

Q13137

Site	PTM Type	Source
K87	Ubiquitination	Uniprot
K94	Ubiquitination	Uniprot
Y96	Phosphorylation	Uniprot
K160	Ubiquitination	Uniprot
K168	Ubiquitination	Uniprot
K180	Ubiquitination	Uniprot
K192	Ubiquitination	Uniprot
Y204	Phosphorylation	Uniprot
K213	Ubiquitination	Uniprot
K225	Ubiquitination	Uniprot
S238	Phosphorylation	Uniprot
K242	Ubiquitination	Uniprot
K262	Ubiquitination	Uniprot
S271	Phosphorylation	Uniprot
K281	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
K312	Ubiquitination	Uniprot
S315	Phosphorylation	Uniprot
K339	Ubiquitination	Uniprot
S347	Phosphorylation	Uniprot
S355	Phosphorylation	Uniprot
Y358	Phosphorylation	Uniprot
Y376	Phosphorylation	Uniprot
Y380	Phosphorylation	Uniprot
S381	Phosphorylation	Uniprot
S387	Phosphorylation	Uniprot
S388	Phosphorylation	Uniprot
S390	Phosphorylation	Uniprot
K395	Ubiquitination	Uniprot
K396	Ubiquitination	Uniprot
K401	Ubiquitination	Uniprot
S445	Phosphorylation	Uniprot

Research Backgrounds

Q13137_CACO2_HUMAN

Function:

Xenophagy-specific receptor required for autophagy-mediated intracellular bacteria degradation. Acts as an effector protein of galectin-sensed membrane damage that restricts the proliferation of infecting pathogens such as Salmonella typhimurium upon entry into the cytosol by targeting LGALS8-associated bacteria for autophagy. Initially orchestrates bacteria targeting to autophagosomes and subsequently ensures pathogen degradation by regulating pathogen-containing autophagosome maturation. Bacteria targeting to autophagosomes relies on its interaction with MAP1LC3A, MAP1LC3B and/or GABARAPL2, whereas regulation of pathogen-containing autophagosome maturation requires the interaction with MAP3LC3C. May play a role in ruffle formation and actin cytoskeleton organization and seems to negatively regulate constitutive secretion.

Subcellular Location:

Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton. Cytoplasmic vesicle>Autophagosome membrane>Peripheral membrane protein.
Note: According to PubMed:7540613, localizes to nuclear dots. According to PubMed:9230084 and PubMed:12869526, it is not a nuclear dot-associated protein but localizes predominantly in the cytoplasm with a coarse-grained distribution preferentially close to the nucleus.

Tissue Specificity:

Expressed in all tissues tested with highest expression in skeletal muscle and lowest in brain.

Subunit Structure:

Dimer. Part of a complex consisting of CALCOCO2, TAX1BP1 and MYO6. Interacts with GEMIN4. Interacts with ATG8 family members MAP1LC3A, MAP1LC3B, GABARAP, GABARAPL1 and GABARAPL2. Interacts with ATG8 family member MAP1LC3C. Interacts with LGALS8.

Family&Domains:

The MYO6-binding domain is required for autophagy-mediated degradation of infecting bacteria such as Salmonella typhimurium, but not for bacteria targeting to autophagosomes.

The CLIR (LC3C-interacting region) motif is required for interaction with MAP1LC3C, but dispensable for CALCOCO2-mediated autophagosome maturation.

The LIR-like motif is required for interaction with MAP1LC3A, MAP1LC3B and GABARAPL2, as well as for CALCOCO2-mediated autophagosome maturation.

The LGALS8-binding domain is essential for the recruitment to cytosol-exposed infecting bacteria.

Belongs to the CALCOCO family.

References

1). SFTSV nucleoprotein mediates DNA sensor cGAS degradation to suppress cGAS-dependent antiviral responses. Microbiology spectrum, 2024 (PubMed: 38712963) [IF=3.7]

2). SFTSV nucleoprotein mediates DNA sensor cGAS degradation to evade cGAS-dependent antiviral responses.. Research Square, 2023

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CALCOCO2 Antibody - #DF12360