C14orf166 Antibody - #DF12359
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12359, RRID:AB_2845164.
Fold/Unfold
C14orf166; CGI 99; CGI-99; CGI99; Chromosome 14 open reading frame 166; CLE; CLE7; CN166_HUMAN; Homeobox prox 1; LCRP369; RLL motif containing 1; RLLM1; UPF0568 protein C14orf166;
Immunogens
Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors.
- Q9Y224 RTRAF_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MFRRKLTALDYHNPAGFNCKDETEFRNFIVWLEDQKIRHYKIEDRGNLRNIHSSDWPKFFEKYLRDVNCPFKIQDRQEAIDWLLGLAVRLEYGDNAEKYKDLVPDNSKTADNATKNAEPLINLDVNNPDFKAGVMALANLLQIQRHDDYLVMLKAIRILVQERLTQDAVAKANQTKEGLPVALDKHILGFDTGDAVLNEAAQILRLLHIEELRELQTKINEAIVAVQAIIADPKTDHRLGKVGR
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y224 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| M1 | Acetylation | Uniprot | |
| K5 | Ubiquitination | Uniprot | |
| T7 | Phosphorylation | Uniprot | |
| Y11 | Phosphorylation | Uniprot | |
| C19 | S-Nitrosylation | Uniprot | |
| K20 | Acetylation | Uniprot | |
| K20 | Ubiquitination | Uniprot | |
| Y40 | Phosphorylation | Uniprot | |
| K41 | Acetylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| K58 | Acetylation | Uniprot | |
| K58 | Ubiquitination | Uniprot | |
| K62 | Acetylation | Uniprot | |
| R65 | Methylation | Uniprot | |
| K72 | Methylation | Uniprot | |
| K72 | Ubiquitination | Uniprot | |
| K98 | Acetylation | Uniprot | |
| K98 | Ubiquitination | Uniprot | |
| K100 | Ubiquitination | Uniprot | |
| S107 | Phosphorylation | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K115 | Ubiquitination | Uniprot | |
| K131 | Acetylation | Uniprot | |
| K131 | Sumoylation | Uniprot | |
| Y149 | Phosphorylation | Uniprot | |
| K154 | Ubiquitination | Uniprot | |
| K171 | Ubiquitination | Uniprot | |
| K176 | Ubiquitination | Uniprot | |
| K185 | Ubiquitination | Uniprot | |
| K218 | Ubiquitination | Uniprot | |
| K234 | Ubiquitination | Uniprot |
Research Backgrounds
RNA-binding protein involved in modulation of mRNA transcription by Polymerase II. Component of the tRNA-splicing ligase complex and is required for tRNA ligation. May be required for RNA transport.
(Microbial infection) In case of infection by influenza virus A (IVA), is involved in viral replication.
Nucleus. Cytoplasm>Cytosol. Cytoplasm>Perinuclear region. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: May localize at the centrosome during mitosis (PubMed:15147888). Shuttles between the cytosol and the nucleus: enters into the nucleus in case of active transcription while it accumulates in cytosol when transcription level is low (PubMed:24608264).
Nucleus. Cytoplasm.
Note: (Microbial infection) Following influenza A virus (IAV) infection, included in influenza A virions via its association with packaged viral ribonucleoproteins (vRNP) in the nucleus and cytoplasm (PubMed:21900157, PubMed:26864902).
Widely expressed. Expressed at high level in heart and skeletal muscle. Expressed at intermediate level in liver, pancreas, fetal brain and fetal lung. Weakly expressed in adult brain, adult lung, placenta, fetal liver and fetal kidney. Overexpressed in many brain tumors.
Homodimer (Probable). Interacts with FAM98A (via N- and C-terminus). Interacts with NIN; which may prevent phosphorylation of NIN. Interacts with POLR2A. Component of a tRNA-splicing ligase complex with FAM98B, DDX1 and RTCB.
(Microbial infection) Interacts with influenza A virus (IAV) RNA polymerase subunits PA, PB1 and PB2, and nucleocapsid NP. Associates with IAV polymerase complexes both in the nucleus and cytosol. Associates with IAV ribonucleoproteins (vRNP) packaged in virions. Interacts with hepatitis C virus core protein p19.
Belongs to the RTRAF family.
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