ARL8B Antibody - #DF12350

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Product: ARL8B Antibody
Catalog: DF12350
Description: Rabbit polyclonal antibody to ARL8B
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 22 kDa; 22kD(Calculated).
Uniprot: Q9NVJ2
RRID: AB_2845155

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
ARL8B Antibody detects endogenous levels of total ARL8B.
RRID:
AB_2845155
Cite Format: Affinity Biosciences Cat# DF12350, RRID:AB_2845155.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADP ribosylation factor like 10C; ADP-ribosylation factor-like protein 10C; ADP-ribosylation factor-like protein 8B; ARL10C; ARL8B; ARL8B_HUMAN; FLJ10702; Gie1; Novel small G protein indispensable for equal chromosome segregation 1;

Immunogens

Immunogen:
Uniprot:

Q9NVJ2(ARL8B_HUMAN) >>Visit HPA database.

Gene(ID):
ARL8B(55207)
Expression:
Q9NVJ2 ARL8B_HUMAN:

Ubiquitously expressed.

Sequence:
MLALISRLLDWFRSLFWKEEMELTLVGLQYSGKTTFVNVIASGQFSEDMIPTVGFNMRKVTKGNVTIKIWDIGGQPRFRSMWERYCRGVNAIVYMIDAADREKIEASRNELHNLLDKPQLQGIPVLVLGNKRDLPNALDEKQLIEKMNLSAIQDREICCYSISCKEKDNIDITLQWLIQHSKSRRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NVJ2 As Substrate

Site PTM Type Enzyme
M1 Acetylation
T61 Phosphorylation
K62 Ubiquitination
K68 Ubiquitination
S107 Phosphorylation
K117 Ubiquitination
K141 Sumoylation
K141 Ubiquitination
K146 Ubiquitination
K182 Ubiquitination

Research Backgrounds

Function:

Plays a role in lysosome motility. In neurons, mediates the anterograde axonal long-range transport of presynaptic lysosome-related vesicles required for presynaptic biogenesis and synaptic function (By similarity). May play a role in chromosome segregation.

Subcellular Location:

Late endosome membrane. Lysosome membrane. Cytoplasm>Cytoskeleton>Spindle. Cell projection>Axon. Cell junction>Synapse.
Note: Localizes with microtubules at the spindle mid-zone during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Interacts with tubulin Ref.16). Interacts with BORCS5; recruits ARL8B to lysosomes.

Family&Domains:

Belongs to the small GTPase superfamily. Arf family.

References

1). The role of lysosomes as intermediates in betacoronavirus PHEV egress from nerve cells. Journal of virology, 2023 (PubMed: 38009916) [IF=4.0]

Application: WB    Species: Mouse    Sample:

Fig 3 PHEV uses an Arl8b-dependent lysosomal exocytic pathway to egress. (A) Colocalization of PHEV and surface LAMP1. Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red) and anti-PHEV (green) antibodies. Scale bar, 10 µm. (B) The surface LAMP1 levels on mock- and PHEV-infected cells from the experiment whose results are shown in panel A were quantified by using Image J. (C) The protein levels of Pro-CTSD, Pro-CTSB, Mat-CTSD, Mat-CTSB, and GAPDH at 24 and 48 hpi were analyzed by western blot, respectively. (D) Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red), anti-Arl8b (green), and anti-PHEV (teal) antibodies. Scale bar, 10 µm. (E) The protein levels of Arl8b, PHEV, and GAPDH were analyzed by western blot in PHEV-infected Arl8b small interfering RNA (siRNA)-treated cells or PHEV-infected-non-target siRNA-treated cells. (F) The PHEV N genomic RNA was determined using qPCR in Arl8b siRNA-treated cells and non-target siRNA-treated cells. The data were normalized to the non-target siRNA-treated cells. Representative blots and images are shown. Data are shown as mean ± SD. P values were considered significant when P < 0.05 and denoted as

Application: IF/ICC    Species: Mouse    Sample:

Fig 3 PHEV uses an Arl8b-dependent lysosomal exocytic pathway to egress. (A) Colocalization of PHEV and surface LAMP1. Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red) and anti-PHEV (green) antibodies. Scale bar, 10 µm. (B) The surface LAMP1 levels on mock- and PHEV-infected cells from the experiment whose results are shown in panel A were quantified by using Image J. (C) The protein levels of Pro-CTSD, Pro-CTSB, Mat-CTSD, Mat-CTSB, and GAPDH at 24 and 48 hpi were analyzed by western blot, respectively. (D) Mock- or PHEV-infected cells at 48 hpi were immunostained with anti-LAMP1 (red), anti-Arl8b (green), and anti-PHEV (teal) antibodies. Scale bar, 10 µm. (E) The protein levels of Arl8b, PHEV, and GAPDH were analyzed by western blot in PHEV-infected Arl8b small interfering RNA (siRNA)-treated cells or PHEV-infected-non-target siRNA-treated cells. (F) The PHEV N genomic RNA was determined using qPCR in Arl8b siRNA-treated cells and non-target siRNA-treated cells. The data were normalized to the non-target siRNA-treated cells. Representative blots and images are shown. Data are shown as mean ± SD. P values were considered significant when P < 0.05 and denoted as

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