Product: Aconitase 2 Antibody
Catalog: DF12346
Description: Rabbit polyclonal antibody to Aconitase 2
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 85 kDa; 85kD(Calculated).
Uniprot: Q99798
RRID: AB_2845151

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(91%), Horse(91%), Sheep(91%), Rabbit(91%), Dog(91%), Chicken(100%), Xenopus(90%)
Clonality:
Polyclonal
Specificity:
Aconitase 2 Antibody detects endogenous levels of total Aconitase 2.
RRID:
AB_2845151
Cite Format: Affinity Biosciences Cat# DF12346, RRID:AB_2845151.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ACO 2; ACO2; ACON_HUMAN; aconitase 2; Aconitase 2 mitochondrial; Aconitase; Aconitase2; Aconitate hydratase; Aconitate hydratase mitochondrial; ACONM; Citrate hydro lyase; Citrate hydro-lyase; ICRD; mitochondrial;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAPYSLLVTRLQKALGVRQYHVASVLCQRAKVAMSHFEPNEYIHYDLLEKNINIVRKRLNRPLTLSEKIVYGHLDDPASQEIERGKSYLRLRPDRVAMQDATAQMAMLQFISSGLSKVAVPSTIHCDHLIEAQVGGEKDLRRAKDINQEVYNFLATAGAKYGVGFWKPGSGIIHQIILENYAYPGVLLIGTDSHTPNGGGLGGICIGVGGADAVDVMAGIPWELKCPKVIGVKLTGSLSGWSSPKDVILKVAGILTVKGGTGAIVEYHGPGVDSISCTGMATICNMGAEIGATTSVFPYNHRMKKYLSKTGREDIANLADEFKDHLVPDPGCHYDQLIEINLSELKPHINGPFTPDLAHPVAEVGKVAEKEGWPLDIRVGLIGSCTNSSYEDMGRSAAVAKQALAHGLKCKSQFTITPGSEQIRATIERDGYAQILRDLGGIVLANACGPCIGQWDRKDIKKGEKNTIVTSYNRNFTGRNDANPETHAFVTSPEIVTALAIAGTLKFNPETDYLTGTDGKKFRLEAPDADELPKGEFDPGQDTYQHPPKDSSGQHVDVSPTSQRLQLLEPFDKWDGKDLEDLQILIKVKGKCTTDHISAAGPWLKFRGHLDNISNNLLIGAINIENGKANSVRNAVTQEFGPVPDTARYYKKHGIRWVVIGDENYGEGSSREHAALEPRHLGGRAIITKSFARIHETNLKKQGLLPLTFADPADYNKIHPVDKLTIQGLKDFTPGKPLKCIIKHPNGTQETILLNHTFNETQIEWFRAGSALNRMKELQQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Pig
91
Horse
91
Bovine
91
Sheep
91
Dog
91
Rabbit
91
Xenopus
90
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q99798 As Substrate

Site PTM Type Enzyme
T9 Phosphorylation
Y42 Phosphorylation
K50 Acetylation
Y71 Phosphorylation P09769 (FGR)
S79 Phosphorylation
S87 Phosphorylation
K144 Acetylation
K144 Ubiquitination
Y151 Phosphorylation
K233 Acetylation
S237 Phosphorylation
S242 Phosphorylation
S243 Phosphorylation
K245 Ubiquitination
K309 Ubiquitination
T310 Phosphorylation
K370 Acetylation
K370 Ubiquitination
Y390 Phosphorylation
K401 Ubiquitination
K409 Ubiquitination
C410 S-Nitrosylation
K411 Ubiquitination
Y432 Phosphorylation
T467 Phosphorylation
T470 Phosphorylation
S471 Phosphorylation
R474 Methylation
T477 Phosphorylation
Y513 Phosphorylation
T515 Phosphorylation
K520 Acetylation
K520 Ubiquitination
K521 Acetylation
R523 Methylation
K534 Acetylation
K534 Ubiquitination
T543 Phosphorylation
Y544 Phosphorylation P09769 (FGR)
K549 Acetylation
S559 Phosphorylation
K573 Acetylation
K573 Ubiquitination
K577 Acetylation
C592 S-Nitrosylation
K605 Acetylation
K605 Ubiquitination
T646 Phosphorylation
Y665 Phosphorylation P09769 (FGR)
S669 Phosphorylation
S670 Phosphorylation
R679 Methylation
K689 Acetylation
K689 Ubiquitination
K700 Acetylation
K701 Ubiquitination
K723 Ubiquitination
K730 Acetylation
K730 Ubiquitination
K743 Acetylation

Research Backgrounds

Function:

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.

Subcellular Location:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Monomer.

Family&Domains:

Belongs to the aconitase/IPM isomerase family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.