URAT1 Antibody - #DF12340

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Product: URAT1 Antibody
Catalog: DF12340
Description: Rabbit polyclonal antibody to URAT1
Application: WB IHC
Reactivity: Human, Mouse, Rat, Pig, Monkey
Prediction: Pig, Horse
Mol.Wt.: 40 kDa,65-70 kDa; 60kD(Calculated).
Uniprot: Q96S37
RRID: AB_2845145

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Pig,Monkey
Prediction:
Horse(91%)
Clonality:
Polyclonal
Specificity:
URAT1 Antibody detects endogenous levels of total URAT1.
RRID:
AB_2845145
Cite Format: Affinity Biosciences Cat# DF12340, RRID:AB_2845145.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Organic anion transporter 4-like protein; Renal-specific transporter; RST; S22AC_HUMAN; Slc22a12; Solute carrier family 22 member 12; Urate anion exchanger 1;

Immunogens

Immunogen:
Uniprot:

Q96S37(S22AC_HUMAN) >>Visit HPA database.

Gene(ID):
SLC22A12(116085)
Expression:
Q96S37 S22AC_HUMAN:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

Sequence:
MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
91
Pig
82
Bovine
73
Dog
73
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Detected in the luminal membrane of the epithelium of renal proximal tubules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

Subunit Structure:

Interacts with PDZK1.

Family&Domains:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

References

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE (PubMed: 35714456) [IF=7.9]
2). Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice. Food & Function (PubMed: 34606558) [IF=6.1]
3). The Extract of Sonneratia apetala Leaves and Branches Ameliorates Hyperuricemia in Mice by Regulating Renal Uric Acid Transporters and Suppressing the Activation of the JAK/STAT Signaling Pathway. Frontiers in Pharmacology (PubMed: 34483901) [IF=5.6]

Application: WB    Species: Mice    Sample: kidney tissues

FIGURE 10 Effects of SAL on the expression of uric acid transporters. (A) The expression levels of the URAT1, GLUT9, and OAT1; quantitative results of Western blot analyses for (B) GLUT9; (C) URAT1; (D) OAT1. β-actin was used as a loading control. All data are expressed as mean ± SD (n = 3). ## p < 0.01 vs. the intact group, **p < 0.01 vs. the vehicle group.
4). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology (PubMed: 34699754) [IF=5.0]
5). Naringenin Attenuates Gout-Induced Renal Injury in Rats Via TLR4/NLRP3 Pathway and Urate Transporters.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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