URAT1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from VERO cells, using URAT1 Antibody.

FIGURE 10 Effects of SAL on the expression of uric acid transporters.

Product:	URAT1 Antibody
Catalog:	DF12340
Description:	Rabbit polyclonal antibody to URAT1
Application:	WB IHC
Reactivity:	Human, Mouse, Rat, Pig, Monkey
Prediction:	Pig, Horse
Mol.Wt.:	40 kDa,65-70 kDa; 60kD(Calculated).
Uniprot:	Q96S37
RRID:	AB_2845145

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Pig,Monkey

Prediction:

Horse(91%)

Clonality:

Polyclonal

Specificity:

URAT1 Antibody detects endogenous levels of total URAT1.

RRID:

AB_2845145
Cite Format: Affinity Biosciences Cat# DF12340, RRID:AB_2845145.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Organic anion transporter 4-like protein; Renal-specific transporter; RST; S22AC_HUMAN; Slc22a12; Solute carrier family 22 member 12; Urate anion exchanger 1;

Immunogens

Immunogen:

Uniprot:

Q96S37(S22AC_HUMAN) >>Visit HPA database.

Gene(ID):

SLC22A12(116085)

Expression:

Q96S37 S22AC_HUMAN:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

Sequence:

Q96S37 S22AC_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFSELLDLVGGLGRFQVLQTMALMVSIMWLCTQSMLENFSAAVPSHRCWAPLLDNSTAQASILGSLSPEALLAISIPPGPNQRPHQCRRFRQPQWQLLDPNATATSWSEADTEPCVDGWVYDRSIFTSTIVAKWNLVCDSHALKPMAQSIYLAGILVGAAACGPASDRFGRRLVLTWSYLQMAVMGTAAAFAPAFPVYCLFRFLLAFAVAGVMMNTGTLLMEWTAARARPLVMTLNSLGFSFGHGLTAAVAYGVRDWTLLQLVVSVPFFLCFLYSWWLAESARWLLTTGRLDWGLQELWRVAAINGKGAVQDTLTPEVLLSAMREELSMGQPPASLGTLLRMPGLRFRTCISTLCWFAFGFTFFGLALDLQALGSNIFLLQMFIGVVDIPAKMGALLLLSHLGRRPTLAASLLLAGLCILANTLVPHEMGALRSALAVLGLGGVGAAFTCITIYSSELFPTVLRMTAVGLGQMAARGGAILGPLVRLLGVHGPWLPLLVYGTVPVLSGLAALLLPETQSLPLPDTIQDVQNQAVKKATHGTLGNSVLKSTQF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

Pig

Bovine

Dog

Sheep

Xenopus

Zebrafish

Chicken

Rabbit

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q96S37_S22AC_HUMAN

Function:

Required for efficient urate re-absorption in the kidney. Regulates blood urate levels. Mediates saturable urate uptake by facilitating the exchange of urate against organic anions.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Detected in the luminal membrane of the epithelium of renal proximal tubules.

Tissue Specificity:

Detected in kidney (at protein level). Detected in fetal and adult kidney. Detected in epithelial cells of proximal tubules in renal cortex.

Subunit Structure:

Interacts with PDZK1.

Family&Domains:

Belongs to the major facilitator (TC 2.A.1) superfamily. Organic cation transporter (TC 2.A.1.19) family.

References

1). Caffeic acid phenethyl ester alleviated hypouricemia in hyperuricemic mice through inhibiting XOD and up-regulating OAT3. PHYTOMEDICINE, 2022 (PubMed: 35714456) [IF=7.9]

2). Sonneratia apetala seed oil attenuates potassium oxonate/hypoxanthine-induced hyperuricemia and renal injury in mice. Food & Function, 2021 (PubMed: 34606558) [IF=6.1]

3). The Extract of Sonneratia apetala Leaves and Branches Ameliorates Hyperuricemia in Mice by Regulating Renal Uric Acid Transporters and Suppressing the Activation of the JAK/STAT Signaling Pathway. Frontiers in Pharmacology, 2021 (PubMed: 34483901) [IF=5.6]

Application: WB Species: Mice Sample: kidney tissues

FIGURE 10 Effects of SAL on the expression of uric acid transporters. (A) The expression levels of the URAT1, GLUT9, and OAT1; quantitative results of Western blot analyses for (B) GLUT9; (C) URAT1; (D) OAT1. β-actin was used as a loading control. All data are expressed as mean ± SD (n = 3). ## p < 0.01 vs. the intact group, **p < 0.01 vs. the vehicle group.

4). Berberrubine attenuates potassium oxonate- and hypoxanthine-induced hyperuricemia by regulating urate transporters and JAK2/STAT3 signaling pathway. European Journal of Pharmacology, 2021 (PubMed: 34699754) [IF=5.0]

5). Ethanol extract of Nepeta angustifoia C. Y. Wu ameliorates hyperuricemia in fructose-induced mice. Pakistan journal of pharmaceutical sciences, 2022 (PubMed: 36008897) [IF=0.8]

6). Naringenin Attenuates Gout-Induced Renal Injury in Rats Via TLR4/NLRP3 Pathway and Urate Transporters. , 2023

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URAT1 Antibody - #DF12340