TMSB4X Antibody - #DF12334

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Product: TMSB4X Antibody
Catalog: DF12334
Description: Rabbit polyclonal antibody to TMSB4X
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Chicken, Xenopus
Mol.Wt.: 5 kDa; 5kD(Calculated).
Uniprot: P62328
RRID: AB_2845139

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
TMSB4X Antibody detects endogenous levels of total TMSB4X.
RRID:
AB_2845139
Cite Format: Affinity Biosciences Cat# DF12334, RRID:AB_2845139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Fx; Hematopoietic system regulatory peptide; Prothymosin beta 4; PTMB 4; PTMB4; Seraspenide; T beta 4; T beta-4; TB4X; THYB 4; Thyb4; Thymosin beta 4; Thymosin beta 4 X chromosome; Thymosin beta 4 X linked; TMSB 4; TMSB4; Tmsb4x; TYB4_HUMAN;

Immunogens

Immunogen:
Uniprot:

P62328(TYB4_HUMAN) >>Visit HPA database.

Gene(ID):
TMSB4X(7114)
Expression:
P62328 TYB4_HUMAN:

Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.

Sequence:
MSDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Xenopus
100
Chicken
100
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P62328 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
K4 Acetylation
K4 Methylation
K4 Ubiquitination
K12 Acetylation
K12 Ubiquitination
K15 Acetylation
S16 Phosphorylation
K17 Acetylation
K20 Ubiquitination
T21 Phosphorylation
T23 Phosphorylation
K26 Acetylation
K26 Ubiquitination
S31 Phosphorylation
K32 Acetylation
K32 Ubiquitination
T34 Phosphorylation
K39 Acetylation
K39 Ubiquitination
S44 Phosphorylation

Research Backgrounds

Function:

Plays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization.

Seraspenide inhibits the entry of hematopoietic pluripotent stem cells into the S-phase.

Subcellular Location:

Cytoplasm>Cytoskeleton.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in several hemopoietic cell lines and lymphoid malignant cells. Decreased levels in myeloma cells.

Subunit Structure:

Interacts with SERPINB1 (By similarity). Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X.

Family&Domains:

Belongs to the thymosin beta family.

Research Fields

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

References

1). Fabrication of Tβ4-Exosome-releasing artificial stem cells for myocardial infarction therapy by improving coronary collateralization. Bioactive Materials, 2022 (PubMed: 35386821) [IF=18.9]

Application: WB    Species: mouse    Sample: MSCs and Tβ4-MSCs

Fig. 2.| Preparation and characterization of Tβ4-ASCs. (a) The expression of Tβ4 in MSCs and Tβ4-MSCs was detected via Western blot respectively. *P < 0.05.
2). Thymosin β4 prevents oxygen-glucose deprivation/reperfusion-induced injury in rat cortical neurons. Neuropsychiatric Disease and Treatment, 2019 (PubMed: 31692484) [IF=3.2]

Application: WB    Species: rat    Sample: cortical neurons

Figure 2| Evaluation of transfection efficiency. (A, B) Transfection of the Tmsb4x overexpression plasmid increased Tβ4 mRNA (A) and protein (B) levels in cortical neurons. Data shown as mean ± SD. *P<0.05 vs control.

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