Product: Syntaxin 16 Antibody
Catalog: DF12330
Description: Rabbit polyclonal antibody to Syntaxin 16
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Dog
Mol.Wt.: 42 kDa; 37kD(Calculated).
Uniprot: O14662
RRID: AB_2845135

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
Syntaxin 16 Antibody detects endogenous levels of total Syntaxin 16.
RRID:
AB_2845135
Cite Format: Affinity Biosciences Cat# DF12330, RRID:AB_2845135.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

hsyn16; MGC90328; Stx16; STX16_HUMAN; Syn16; Syntaxin-16;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
O14662 STX16_HUMAN:

Ubiquitous.

Sequence:
MATRRLTDAFLLLRNNSIQNRQLLAEQVSSHITSSPLHSRSIAAELDELADDRMALVSGISLDPEAAIGVTKRPPPKWVDGVDEIQYDVGRIKQKMKELASLHDKHLNRPTLDDSSEEEHAIEITTQEITQLFHRCQRAVQALPSRARACSEQEGRLLGNVVASLAQALQELSTSFRHAQSGYLKRMKNREERSQHFFDTSVPLMDDGDDNTLYHRGFTEDQLVLVEQNTLMVEEREREIRQIVQSISDLNEIFRDLGAMIVEQGTVLDRIDYNVEQSCIKTEDGLKQLHKAEQYQKKNRKMLVILILFVIIIVLIVVLVGVKSR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Dog
100
Chicken
77
Pig
75
Horse
0
Sheep
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O14662 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
S17 Phosphorylation
S35 Phosphorylation
S41 Phosphorylation
S61 Phosphorylation
T71 Phosphorylation
K77 Ubiquitination
Y87 Phosphorylation
K97 Methylation
T200 Phosphorylation
T212 Phosphorylation
Y214 Phosphorylation
K281 Ubiquitination
K287 Ubiquitination
K301 Ubiquitination

Research Backgrounds

Function:

SNARE involved in vesicular transport from the late endosomes to the trans-Golgi network.

Subcellular Location:

Golgi apparatus membrane>Single-pass type IV membrane protein.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Subunit Structure:

Interacts with GCC2. Interacts with BAIAP3; this interaction is increased in the presence of calcium.

Family&Domains:

Belongs to the syntaxin family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > SNARE interactions in vesicular transport.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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