Pax-5 Antibody | Affinity Biosciences

WB
IF/ICC

1/2

Western blot analysis on 293 cell lysates using Pax-5 Antibody.

Product:	Pax-5 Antibody
Catalog:	AF0238
Description:	Rabbit polyclonal antibody to Pax-5
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Horse, Rabbit, Dog
Mol.Wt.:	40kDa; 42kD(Calculated).
Uniprot:	Q02548
RRID:	AB_2833413

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(93%), Horse(86%), Rabbit(93%), Dog(86%)

Clonality:

Polyclonal

Specificity:

Pax-5 Antibody detects endogenous levels of total Pax-5.

RRID:

AB_2833413
Cite Format: Affinity Biosciences Cat# AF0238, RRID:AB_2833413.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

B cell lineage specific activator; B cell lineage specific activator protein; B cell specific activator protein; B cell specific transcription factor; B-cell-specific transcription factor; BSAP; EBB-1; KLP; Paired box 5; Paired box gene 5 (B cell lineage specific activator protein); Paired box gene 5 (B cell lineage specific activator); Paired box gene 5; Paired box homeotic gene 5; Paired box protein Pax 5; Paired box protein Pax-5; Paired domain gene 5; PAX 5; PAX5; PAX5_HUMAN; Transcription factor PAX 5;

Immunogens

Immunogen:

Uniprot:

Q02548(PAX5_HUMAN) >>Visit HPA database.

Gene(ID):

PAX5(5079)

Description:

AX5 May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene. Interacts with DAXX. Binds DNA as a monomer. Binds TLE4.

Sequence:

Q02548 PAX5_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MDLEKNYPTPRTSRTGHGGVNQLGGVFVNGRPLPDVVRQRIVELAHQGVRPCDISRQLRVSHGCVSKILGRYYETGSIKPGVIGGSKPKVATPKVVEKIAEYKRQNPTMFAWEIRDRLLAERVCDNDTVPSVSSINRIIRTKVQQPPNQPVPASSHSIVSTGSVTQVSSVSTDSAGSSYSISGILGITSPSADTNKRKRDEGIQESPVPNGHSLPGRDFLRKQMRGDLFTQQQLEVLDRVFERQHYSDIFTTTEPIKPEQTTEYSAMASLAGGLDDMKANLASPTPADIGSSVPGPQSYPIVTGRDLASTTLPGYPPHVPPAGQGSYSAPTLTGMVPGSEFSGSPYSHPQYSSYNDSWRFPNPGLLGSPYYYSAAARGAAPPAAATAYDRH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Dog

Chicken

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q02548 As Substrate

Q02548

Site	PTM Type	Source
T9	Phosphorylation	Uniprot
T15	Phosphorylation	Uniprot
Y102	Phosphorylation	Uniprot
S133	Phosphorylation	Uniprot
S168	Phosphorylation	Uniprot
Y179	Phosphorylation	Uniprot
S180	Phosphorylation	Uniprot
S206	Phosphorylation	Uniprot
S298	Phosphorylation	Uniprot
Y299	Phosphorylation	Uniprot
R377	Methylation	Uniprot

Research Backgrounds

Q02548_PAX5_HUMAN

Function:

May play an important role in B-cell differentiation as well as neural development and spermatogenesis. Involved in the regulation of the CD19 gene, a B-lymphoid-specific target gene.

PTMs:

O-glycosylated.

Subcellular Location:

Nucleus.

Subunit Structure:

Interacts with DAXX (By similarity). Binds DNA as a monomer. Binds TLE4. Interacts with ETS1, altering its DNA-binding properties.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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Pax-5 Antibody - #AF0238