PYGB Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat brain tissue, using PYGB antibody.

DF12308 at 1/100 staining Rat heart tissue by IHC-P.

Product:	PYGB Antibody
Catalog:	DF12308
Description:	Rabbit polyclonal antibody to PYGB
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Prediction:	Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.:	97 kDa; 97kD(Calculated).
Uniprot:	P11216
RRID:	AB_2845113

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)

Clonality:

Polyclonal

Specificity:

PYGB Antibody detects endogenous levels of total PYGB.

RRID:

AB_2845113
Cite Format: Affinity Biosciences Cat# DF12308, RRID:AB_2845113.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Brain glycogen phosphorylase; Glycogen phosphorylase B; Glycogen phosphorylase brain form; Glycogen Phosphorylase Isoenzyme BB; Glycogen phosphorylase, brain form; MGC9213; Phosphorylase glycogen brain; PYGB; PYGB_HUMAN;

Immunogens

Immunogen:

Uniprot:

P11216(PYGB_HUMAN) >>Visit HPA database.

Gene(ID):

PYGB(5834)

Sequence:

P11216 PYGB_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAKPLTDSEKRKQISVRGLAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHTPDGVKWLDTQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLQDFNVGDYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Horse

100

Bovine

100

Sheep

100

Dog

100

Xenopus

100

Zebrafish

100

Chicken

100

Rabbit

100

Pig

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11216 As Substrate

P11216

Site	PTM Type	Enzyme	Source
A2	Acetylation		Uniprot
K3	Acetylation		Uniprot
K3	Ubiquitination		Uniprot
T6	Phosphorylation		Uniprot
S8	Phosphorylation		Uniprot
K10	Acetylation		Uniprot
K10	Ubiquitination		Uniprot
S15	Phosphorylation	Q8IWU2 (LMTK2)	Uniprot
S31	Phosphorylation		Uniprot
T39	Phosphorylation		Uniprot
K42	Ubiquitination		Uniprot
Y52	Phosphorylation		Uniprot
Y75	Phosphorylation		Uniprot
Y76	Phosphorylation		Uniprot
Y84	Phosphorylation		Uniprot
Y85	Phosphorylation		Uniprot
S87	Phosphorylation		Uniprot
Y91	Phosphorylation		Uniprot
Y162	Phosphorylation		Uniprot
Y186	Phosphorylation		Uniprot
K192	Ubiquitination		Uniprot
Y197	Phosphorylation		Uniprot
Y204	Phosphorylation		Uniprot
T210	Phosphorylation		Uniprot
Y227	Phosphorylation		Uniprot
T229	Phosphorylation		Uniprot
K235	Ubiquitination		Uniprot
T241	Phosphorylation		Uniprot
K248	Ubiquitination		Uniprot
K254	Ubiquitination		Uniprot
Y263	Phosphorylation		Uniprot
Y281	Phosphorylation		Uniprot
K290	Ubiquitination		Uniprot
K295	Ubiquitination		Uniprot
K316	Acetylation		Uniprot
C326	S-Nitrosylation		Uniprot
K359	Ubiquitination		Uniprot
K364	Ubiquitination		Uniprot
K371	Ubiquitination		Uniprot
T372	Phosphorylation		Uniprot
K395	Ubiquitination		Uniprot
Y405	Phosphorylation		Uniprot
S430	Phosphorylation		Uniprot
K438	Ubiquitination		Uniprot
K465	Ubiquitination		Uniprot
K470	Ubiquitination		Uniprot
Y473	Phosphorylation		Uniprot
K479	Ubiquitination		Uniprot
K483	Ubiquitination		Uniprot
K520	Ubiquitination		Uniprot
K521	Ubiquitination		Uniprot
S527	Phosphorylation		Uniprot
K545	Ubiquitination		Uniprot
K552	Ubiquitination		Uniprot
Y554	Phosphorylation		Uniprot
K557	Ubiquitination		Uniprot
S562	Phosphorylation		Uniprot
K597	Ubiquitination		Uniprot
K609	Ubiquitination		Uniprot
K642	Ubiquitination		Uniprot
K656	Ubiquitination		Uniprot
Y727	Phosphorylation		Uniprot
Y732	Phosphorylation		Uniprot
Y733	Phosphorylation		Uniprot
K740	Ubiquitination		Uniprot
S752	Phosphorylation		Uniprot
K754	Ubiquitination		Uniprot
K796	Acetylation		Uniprot
K796	Ubiquitination		Uniprot
K811	Acetylation		Uniprot
K811	Ubiquitination		Uniprot

Research Backgrounds

P11216_PYGB_HUMAN

Function:

Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

PTMs:

Phosphorylated. Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A (By similarity).

Subunit Structure:

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.

Family&Domains:

Belongs to the glycogen phosphorylase family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis. (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway. (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

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PYGB Antibody - #DF12308