Product: PYGB Antibody
Catalog: DF12308
Description: Rabbit polyclonal antibody to PYGB
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 97 kDa; 97kD(Calculated).
Uniprot: P11216
RRID: AB_2845113

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PYGB Antibody detects endogenous levels of total PYGB.
RRID:
AB_2845113
Cite Format: Affinity Biosciences Cat# DF12308, RRID:AB_2845113.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Brain glycogen phosphorylase; Glycogen phosphorylase B; Glycogen phosphorylase brain form; Glycogen Phosphorylase Isoenzyme BB; Glycogen phosphorylase, brain form; MGC9213; Phosphorylase glycogen brain; PYGB; PYGB_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MAKPLTDSEKRKQISVRGLAGLGDVAEVRKSFNRHLHFTLVKDRNVATPRDYFFALAHTVRDHLVGRWIRTQQHYYERDPKRIYYLSLEFYMGRTLQNTMVNLGLQNACDEAIYQLGLDLEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEFGIFNQKIVNGWQVEEADDWLRYGNPWEKARPEYMLPVHFYGRVEHTPDGVKWLDTQVVLAMPYDTPVPGYKNNTVNTMRLWSAKAPNDFKLQDFNVGDYIEAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKSSKFGCRDPVRTCFETFPDKVAIQLNDTHPALSIPELMRILVDVEKVDWDKAWEITKKTCAYTNHTVLPEALERWPVSMFEKLLPRHLEIIYAINQRHLDHVAALFPGDVDRLRRMSVIEEGDCKRINMAHLCVIGSHAVNGVARIHSEIVKQSVFKDFYELEPEKFQNKTNGITPRRWLLLCNPGLADTIVEKIGEEFLTDLSQLKKLLPLVSDEVFIRDVAKVKQENKLKFSAFLEKEYKVKINPSSMFDVHVKRIHEYKRQLLNCLHVVTLYNRIKRDPAKAFVPRTVMIGGKAAPGYHMAKLIIKLVTSIGDVVNHDPVVGDRLKVIFLENYRVSLAEKVIPAADLSQQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGAENLFIFGLRVEDVEALDRKGYNAREYYDHLPELKQAVDQISSGFFSPKEPDCFKDIVNMLMHHDRFKVFADYEAYMQCQAQVDQLYRNPKEWTKKVIRNIACSGKFSSDRTITEYAREIWGVEPSDLQIPPPNIPRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Pig
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P11216 As Substrate

Site PTM Type Enzyme
A2 Acetylation
K3 Acetylation
K3 Ubiquitination
T6 Phosphorylation
S8 Phosphorylation
K10 Acetylation
K10 Ubiquitination
S15 Phosphorylation Q8IWU2 (LMTK2)
S31 Phosphorylation
T39 Phosphorylation
K42 Ubiquitination
Y52 Phosphorylation
Y75 Phosphorylation
Y76 Phosphorylation
Y84 Phosphorylation
Y85 Phosphorylation
S87 Phosphorylation
Y91 Phosphorylation
Y162 Phosphorylation
Y186 Phosphorylation
K192 Ubiquitination
Y197 Phosphorylation
Y204 Phosphorylation
T210 Phosphorylation
Y227 Phosphorylation
T229 Phosphorylation
K235 Ubiquitination
T241 Phosphorylation
K248 Ubiquitination
K254 Ubiquitination
Y263 Phosphorylation
Y281 Phosphorylation
K290 Ubiquitination
K295 Ubiquitination
K316 Acetylation
C326 S-Nitrosylation
K359 Ubiquitination
K364 Ubiquitination
K371 Ubiquitination
T372 Phosphorylation
K395 Ubiquitination
Y405 Phosphorylation
S430 Phosphorylation
K438 Ubiquitination
K465 Ubiquitination
K470 Ubiquitination
Y473 Phosphorylation
K479 Ubiquitination
K483 Ubiquitination
K520 Ubiquitination
K521 Ubiquitination
S527 Phosphorylation
K545 Ubiquitination
K552 Ubiquitination
Y554 Phosphorylation
K557 Ubiquitination
S562 Phosphorylation
K597 Ubiquitination
K609 Ubiquitination
K642 Ubiquitination
K656 Ubiquitination
Y727 Phosphorylation
Y732 Phosphorylation
Y733 Phosphorylation
K740 Ubiquitination
S752 Phosphorylation
K754 Ubiquitination
K796 Acetylation
K796 Ubiquitination
K811 Acetylation
K811 Ubiquitination

Research Backgrounds

Function:

Glycogen phosphorylase that regulates glycogen mobilization. Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties.

PTMs:

Phosphorylated. Phosphorylation of Ser-15 converts phosphorylase B (unphosphorylated) to phosphorylase A (By similarity).

Subunit Structure:

Homodimer. Dimers associate into a tetramer to form the enzymatically active phosphorylase A.

Family&Domains:

Belongs to the glycogen phosphorylase family.

Research Fields

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Human Diseases > Endocrine and metabolic diseases > Insulin resistance.

· Metabolism > Carbohydrate metabolism > Starch and sucrose metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Endocrine system > Insulin signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Glucagon signaling pathway.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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