PRPF8 Antibody - #DF12306

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Product: PRPF8 Antibody
Catalog: DF12306
Description: Rabbit polyclonal antibody to PRPF8
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 220 kDa; 274kD(Calculated).
Uniprot: Q6P2Q9
RRID: AB_2845111

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
PRPF8 Antibody detects endogenous levels of total PRPF8.
RRID:
AB_2845111
Cite Format: Affinity Biosciences Cat# DF12306, RRID:AB_2845111.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

220 kDa U5 snRNP specific protein; 220 kDa U5 snRNP-specific protein; Apoptosis regulated protein 1; Apoptosis regulated protein 2; HPRP8; p220; Pre mRNA processing factor 8; Pre mRNA-processing factor 8, S. cerevisiae, homolog of; Pre-mRNA-processing-splicing factor 8; Precursor mRNA processing protein; PRP8; PRP8 homolog; PRP8 pre mRNA processing factor 8 homolog; PRP8_HUMAN; PRPC8; Prpf8; Retinitis pigmentosa 13 (autosomal dominant); RP13; SNRNP220; Splicing factor Prp8; U5 snRNP specific protein; U5 snRNP specific protein (220 kD), ortholog of S. cerevisiae Prp8p;

Immunogens

Immunogen:
Uniprot:

Q6P2Q9(PRP8_HUMAN) >>Visit HPA database.

Gene(ID):
PRPF8(10594)
Expression:
Q6P2Q9 PRP8_HUMAN:

Widely expressed.

Sequence:
MAGVFPYRGPGNPVPGPLAPLPDYMSEEKLQEKARKWQQLQAKRYAEKRKFGFVDAQKEDMPPEHVRKIIRDHGDMTNRKFRHDKRVYLGALKYMPHAVLKLLENMPMPWEQIRDVPVLYHITGAISFVNEIPWVIEPVYISQWGSMWIMMRREKRDRRHFKRMRFPPFDDEEPPLDYADNILDVEPLEAIQLELDPEEDAPVLDWFYDHQPLRDSRKYVNGSTYQRWQFTLPMMSTLYRLANQLLTDLVDDNYFYLFDLKAFFTSKALNMAIPGGPKFEPLVRDINLQDEDWNEFNDINKIIIRQPIRTEYKIAFPYLYNNLPHHVHLTWYHTPNVVFIKTEDPDLPAFYFDPLINPISHRHSVKSQEPLPDDDEEFELPEFVEPFLKDTPLYTDNTANGIALLWAPRPFNLRSGRTRRALDIPLVKNWYREHCPAGQPVKVRVSYQKLLKYYVLNALKHRPPKAQKKRYLFRSFKATKFFQSTKLDWVEVGLQVCRQGYNMLNLLIHRKNLNYLHLDYNFNLKPVKTLTTKERKKSRFGNAFHLCREVLRLTKLVVDSHVQYRLGNVDAFQLADGLQYIFAHVGQLTGMYRYKYKLMRQIRMCKDLKHLIYYRFNTGPVGKGPGCGFWAAGWRVWLFFMRGITPLLERWLGNLLARQFEGRHSKGVAKTVTKQRVESHFDLELRAAVMHDILDMMPEGIKQNKARTILQHLSEAWRCWKANIPWKVPGLPTPIENMILRYVKAKADWWTNTAHYNRERIRRGATVDKTVCKKNLGRLTRLYLKAEQERQHNYLKDGPYITAEEAVAVYTTTVHWLESRRFSPIPFPPLSYKHDTKLLILALERLKEAYSVKSRLNQSQREELGLIEQAYDNPHEALSRIKRHLLTQRAFKEVGIEFMDLYSHLVPVYDVEPLEKITDAYLDQYLWYEADKRRLFPPWIKPADTEPPPLLVYKWCQGINNLQDVWETSEGECNVMLESRFEKMYEKIDLTLLNRLLRLIVDHNIADYMTAKNNVVINYKDMNHTNSYGIIRGLQFASFIVQYYGLVMDLLVLGLHRASEMAGPPQMPNDFLSFQDIATEAAHPIRLFCRYIDRIHIFFRFTADEARDLIQRYLTEHPDPNNENIVGYNNKKCWPRDARMRLMKHDVNLGRAVFWDIKNRLPRSVTTVQWENSFVSVYSKDNPNLLFNMCGFECRILPKCRTSYEEFTHKDGVWNLQNEVTKERTAQCFLRVDDESMQRFHNRVRQILMASGSTTFTKIVNKWNTALIGLMTYFREAVVNTQELLDLLVKCENKIQTRIKIGLNSKMPSRFPPVVFYTPKELGGLGMLSMGHVLIPQSDLRWSKQTDVGITHFRSGMSHEEDQLIPNLYRYIQPWESEFIDSQRVWAEYALKRQEAIAQNRRLTLEDLEDSWDRGIPRINTLFQKDRHTLAYDKGWRVRTDFKQYQVLKQNPFWWTHQRHDGKLWNLNNYRTDMIQALGGVEGILEHTLFKGTYFPTWEGLFWEKASGFEESMKWKKLTNAQRSGLNQIPNRRFTLWWSPTINRANVYVGFQVQLDLTGIFMHGKIPTLKISLIQIFRAHLWQKIHESIVMDLCQVFDQELDALEIETVQKETIHPRKSYKMNSSCADILLFASYKWNVSRPSLLADSKDVMDSTTTQKYWIDIQLRWGDYDSHDIERYARAKFLDYTTDNMSIYPSPTGVLIAIDLAYNLHSAYGNWFPGSKPLIQQAMAKIMKANPALYVLRERIRKGLQLYSSEPTEPYLSSQNYGELFSNQIIWFVDDTNVYRVTIHKTFEGNLTTKPINGAIFIFNPRTGQLFLKIIHTSVWAGQKRLGQLAKWKTAEEVAALIRSLPVEEQPKQIIVTRKGMLDPLEVHLLDFPNIVIKGSELQLPFQACLKVEKFGDLILKATEPQMVLFNLYDDWLKTISSYTAFSRLILILRALHVNNDRAKVILKPDKTTITEPHHIWPTLTDEEWIKVEVQLKDLILADYGKKNNVNVASLTQSEIRDIILGMEISAPSQQRQQIAEIEKQTKEQSQLTATQTRTVNKHGDEIITSTTSNYETQTFSSKTEWRVRAISAANLHLRTNHIYVSSDDIKETGYTYILPKNVLKKFICISDLRAQIAGYLYGVSPPDNPQVKEIRCIVMVPQWGTHQTVHLPGQLPQHEYLKEMEPLGWIHTQPNESPQLSPQDVTTHAKIMADNPSWDGEKTIIITCSFTPGSCTLTAYKLTPSGYEWGRQNTDKGNNPKGYLPSHYERVQMLLSDRFLGFFMVPAQSSWNYNFMGVRHDPNMKYELQLANPKEFYHEVHRPSHFLNFALLQEGEVYSADREDLYA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Zebrafish
100
Chicken
100
Rabbit
100
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q6P2Q9 As Substrate

Site PTM Type Enzyme
A2 Acetylation
R8 Methylation
S26 Phosphorylation
K29 Ubiquitination
K33 Ubiquitination
K36 Ubiquitination
K43 Acetylation
K43 Ubiquitination
K50 Ubiquitination
K68 Ubiquitination
R79 Methylation
K80 Ubiquitination
K93 Ubiquitination
Y94 Phosphorylation
K218 Ubiquitination
S236 Phosphorylation
T237 Phosphorylation
Y239 Phosphorylation
T247 Phosphorylation
Y254 Phosphorylation
K267 Ubiquitination
K278 Ubiquitination
K301 Ubiquitination
K366 Sumoylation
K366 Ubiquitination
Y394 Phosphorylation
K428 Ubiquitination
K442 Ubiquitination
K449 Ubiquitination
K452 Ubiquitination
K460 Ubiquitination
K480 Ubiquitination
Y501 Phosphorylation
K511 Ubiquitination
K525 Ubiquitination
K528 Ubiquitination
K533 Ubiquitination
K555 Ubiquitination
K609 Acetylation
K609 Ubiquitination
T645 Phosphorylation
K670 Ubiquitination
K674 Ubiquitination
S679 Phosphorylation
K702 Ubiquitination
K705 Ubiquitination
K721 Ubiquitination
K727 Ubiquitination
T733 Phosphorylation
Y742 Phosphorylation
K746 Ubiquitination
K769 Ubiquitination
K785 Ubiquitination
K837 Ubiquitination
K847 Ubiquitination
K853 Ubiquitination
S859 Phosphorylation
Y871 Phosphorylation
K932 Ubiquitination
K941 Ubiquitination
K983 Ubiquitination
K987 Ubiquitination
Y1008 Phosphorylation
T1010 Phosphorylation
K1012 Ubiquitination
K1020 Methylation
K1020 Ubiquitination
S1027 Phosphorylation
Y1028 Phosphorylation
Y1091 Phosphorylation
Y1113 Phosphorylation
Y1128 Phosphorylation
K1131 Ubiquitination
K1144 Ubiquitination
R1151 Methylation
K1158 Ubiquitination
R1201 Methylation
Y1204 Phosphorylation
K1210 Ubiquitination
K1222 Ubiquitination
K1294 Ubiquitination
K1300 Ubiquitination
K1306 Ubiquitination
S1338 Phosphorylation
K1344 Ubiquitination
S1355 Phosphorylation
S1358 Phosphorylation
Y1369 Phosphorylation
K1392 Ubiquitination
R1401 Methylation
T1404 Phosphorylation
R1414 Methylation
R1418 Methylation
K1425 Methylation
K1425 Ubiquitination
Y1432 Phosphorylation
K1434 Ubiquitination
K1443 Methylation
K1443 Ubiquitination
K1449 Ubiquitination
K1463 Acetylation
K1463 Ubiquitination
Y1470 Phosphorylation
S1512 Phosphorylation
K1516 Methylation
K1517 Methylation
S1524 Phosphorylation
S1539 Phosphorylation
T1608 Phosphorylation
K1649 Ubiquitination
Y1671 Phosphorylation
K1732 Ubiquitination
K1735 Ubiquitination
Y1741 Phosphorylation
K1792 Ubiquitination
K1801 Ubiquitination
K1831 Ubiquitination
K1838 Ubiquitination
K1840 Ubiquitination
S1851 Phosphorylation
K1859 Ubiquitination
K1866 Ubiquitination
S1887 Phosphorylation
K1898 Ubiquitination
K1901 Ubiquitination
K1955 Ubiquitination
K1958 Ubiquitination
T1972 Phosphorylation
K1984 Ubiquitination
Y1991 Phosphorylation
K1993 Ubiquitination
K1994 Ubiquitination
K2031 Ubiquitination
K2034 Ubiquitination
S2037 Phosphorylation
T2042 Phosphorylation
K2049 Ubiquitination
S2057 Phosphorylation
Y2062 Phosphorylation
T2064 Phosphorylation
K2070 Ubiquitination
S2079 Phosphorylation
R2086 Methylation
T2087 Phosphorylation
Y2091 Phosphorylation
S2093 Phosphorylation
K2098 Ubiquitination
Y2102 Phosphorylation
K2108 Ubiquitination
K2113 Ubiquitination
K2140 Ubiquitination
S2189 Phosphorylation
K2198 Ubiquitination
S2205 Phosphorylation
K2244 Ubiquitination
K2293 Ubiquitination
Y2294 Phosphorylation

Research Backgrounds

Function:

Plays role in pre-mRNA splicing as core component of precatalytic, catalytic and postcatalytic spliceosomal complexes, both of the predominant U2-type spliceosome and the minor U12-type spliceosome. Functions as a scaffold that mediates the ordered assembly of spliceosomal proteins and snRNAs. Required for the assembly of the U4/U6-U5 tri-snRNP complex, a building block of the spliceosome. Functions as scaffold that positions spliceosomal U2, U5 and U6 snRNAs at splice sites on pre-mRNA substrates, so that splicing can occur. Interacts with both the 5' and the 3' splice site.

Subcellular Location:

Nucleus. Nucleus speckle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed.

Subunit Structure:

Part of the U5 snRNP complex. Component of the U4/U6-U5 tri-snRNP complex composed of the U4, U6 and U5 snRNAs and at least PRPF3, PRPF4, PRPF6, PRPF8, PRPF31, SNRNP200, TXNL4A, SNRNP40, DDX23, CD2BP2, PPIH, SNU13, EFTUD2, SART1 and USP39. Component of the U5.U4atac/U6atac snRNP complexes in U12-dependent spliceosomes. Core component of U2-type precatalytic, catalytic and postcatalytic spliceosomal complexes. Found in a mRNA splicing-dependent exon junction complex (EJC) with SRRM1. Interacts with U5 snRNP proteins SNRP116 and SNRNP40. Interacts with EFTUD2 and SNRNP200. Interacts (via the MPN (JAB/Mov34) domain) with PRPF3 ('Lys-63'-linked polyubiquitinated); may stabilize the U4/U6-U5 tri-snRNP complex. Interacts (via RNase H homology domain) with AAR2. Interacts with RPAP3 and URI1 in a ZNHIT2-dependent manner.

Family&Domains:

The MPN (JAB/Mov34) domain has structural similarity with deubiquitinating enzymes, but lacks the residues that would bind the catalytic metal ion.

Contains a region with structural similarity to reverse transcripase, presenting the classical thumb, fingers and palm architecture, but lacks enzyme activity, since the essential metal-binding residues are not conserved.

Contains a region with structural similarity to type-2 restriction endonucleases, but the residues that would bind catalytic metal ions in endonucleases are instead involved in hydrogen bonds that stabilize the protein structure.

Contains a region with structural similarity to RNase H, but lacks RNase H activity.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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