MYO18A Antibody - #DF12298

(3)  
Product: MYO18A Antibody
Catalog: DF12298
Description: Rabbit polyclonal antibody to MYO18A
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 230 kDa,190 kDa; 233kD(Calculated).
Uniprot: Q92614
RRID: AB_2845103

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(92%), Horse(100%), Sheep(92%), Rabbit(83%), Dog(83%), Chicken(82%)
Clonality:
Polyclonal
Specificity:
MYO18A Antibody detects endogenous levels of total MYO18A.
RRID:
AB_2845103
Cite Format: Affinity Biosciences Cat# DF12298, RRID:AB_2845103.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MAJN; Molecule associated with JAK3 N terminus; Myosin 18A; Myosin containing a PDZ domain; Myosin XVIIIA; MyoXVIIIIA; MYSPDZ; SPR210;

Immunogens

Immunogen:
Uniprot:

Q92614(MY18A_HUMAN) >>Visit HPA database.

Gene(ID):
MYO18A(399687)
Sequence:
MFNLMKKDKDKDGGRKEKKEKKEKKERMSAAELRSLEEMSLRRGFFNLNRSSKRESKTRLEISNPIPIKVASGSDLHLTDIDSDSNRGSVILDSGHLSTASSSDDLKGEEGSFRGSVLQRAAKFGSLAKQNSQMIVKRFSFSQRSRDESASETSTPSEHSAAPSPQVEVRTLEGQLVQHPGPGIPRPGHRSRAPELVTKKFPVDLRLPPVVPLPPPTLRELELQRRPTGDFGFSLRRTTMLDRGPEGQACRRVVHFAEPGAGTKDLALGLVPGDRLVEINGHNVESKSRDEIVEMIRQSGDSVRLKVQPIPELSELSRSWLRSGEGPRREPSDAKTEEQIAAEEAWNETEKVWLVHRDGFSLASQLKSEELNLPEGKVRVKLDHDGAILDVDEDDVEKANAPSCDRLEDLASLVYLNESSVLHTLRQRYGASLLHTYAGPSLLVLGPRGAPAVYSEKVMHMFKGCRREDMAPHIYAVAQTAYRAMLMSRQDQSIILLGSSGSGKTTSCQHLVQYLATIAGISGNKVFSVEKWQALYTLLEAFGNSPTIINGNATRFSQILSLDFDQAGQVASASIQTMLLEKLRVARRPASEATFNVFYYLLACGDGTLRTELHLNHLAENNVFGIVPLAKPEEKQKAAQQFSKLQAAMKVLGISPDEQKACWFILAAIYHLGAAGATKEAAEAGRKQFARHEWAQKAAYLLGCSLEELSSAIFKHQHKGGTLQRSTSFRQGPEESGLGDGTGPKLSALECLEGMAAGLYSELFTLLVSLVNRALKSSQHSLCSMMIVDTPGFQNPEQGGSARGASFEELCHNYTQDRLQRLFHERTFVQELERYKEENIELAFDDLEPPTDDSVAAVDQASHQSLVRSLARTDEARGLLWLLEEEALVPGASEDTLLERLFSYYGPQEGDKKGQSPLLHSSKPHHFLLGHSHGTNWVEYNVTGWLNYTKQNPATQNAPRLLQDSQKKIISNLFLGRAGSATVLSGSIAGLEGGSQLALRRATSMRKTFTTGMAAVKKKSLCIQMKLQVDALIDTIKKSKLHFVHCFLPVAEGWAGEPRSASSRRVSSSSELDLPSGDHCEAGLLQLDVPLLRTQLRGSRLLDAMRMYRQGYPDHMVFSEFRRRFDVLAPHLTKKHGRNYIVVDERRAVEELLECLDLEKSSCCMGLSRVFFRAGTLARLEEQRDEQTSRNLTLFQAACRGYLARQHFKKRKIQDLAIRCVQKNIKKNKGVKDWPWWKLFTTVRPLIEVQLSEEQIRNKDEEIQQLRSKLEKAEKERNELRLNSDRLESRISELTSELTDERNTGESASQLLDAETAERLRAEKEMKELQTQYDALKKQMEVMEMEVMEARLIRAAEINGEVDDDDAGGEWRLKYERAVREVDFTKKRLQQEFEDKLEVEQQNKRQLERRLGDLQADSEESQRALQQLKKKCQRLTAELQDTKLHLEGQQVRNHELEKKQRRFDSELSQAHEEAQREKLQREKLQREKDMLLAEAFSLKQQLEEKDMDIAGFTQKVVSLEAELQDISSQESKDEASLAKVKKQLRDLEAKVKDQEEELDEQAGTIQMLEQAKLRLEMEMERMRQTHSKEMESRDEEVEEARQSCQKKLKQMEVQLEEEYEDKQKVLREKRELEGKLATLSDQVNRRDFESEKRLRKDLKRTKALLADAQLMLDHLKNSAPSKREIAQLKNQLEESEFTCAAAVKARKAMEVEIEDLHLQIDDIAKAKTALEEQLSRLQREKNEIQNRLEEDQEDMNELMKKHKAAVAQASRDLAQINDLQAQLEEANKEKQELQEKLQALQSQVEFLEQSMVDKSLVSRQEAKIRELETRLEFERTQVKRLESLASRLKENMEKLTEERDQRIAAENREKEQNKRLQRQLRDTKEEMGELARKEAEASRKKHELEMDLESLEAANQSLQADLKLAFKRIGDLQAAIEDEMESDENEDLINSLQDMVTKYQKRKNKLEGDSDVDSELEDRVDGVKSWLSKNKGPSKAASDDGSLKSSSPTSYWKSLAPDRSDDEHDPLDNTSRPRYSHSYLSDSDTEAKLTETNA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
92
Sheep
92
Dog
83
Rabbit
83
Chicken
82
Pig
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92614 As Substrate

Site PTM Type Enzyme
S29 Phosphorylation
S35 Phosphorylation
S40 Phosphorylation
S51 Phosphorylation
S52 Phosphorylation
S56 Phosphorylation
S63 Phosphorylation
S72 Phosphorylation
S74 Phosphorylation
T79 Phosphorylation
S83 Phosphorylation
S85 Phosphorylation
S89 Phosphorylation
S94 Phosphorylation
S98 Phosphorylation
T99 Phosphorylation
S101 Phosphorylation
S102 Phosphorylation
S103 Phosphorylation
S112 Phosphorylation
S116 Phosphorylation
S126 Phosphorylation
S132 Phosphorylation
K137 Acetylation
S140 Phosphorylation
S142 Phosphorylation
S145 Phosphorylation
S149 Phosphorylation
S151 Phosphorylation
T153 Phosphorylation
S154 Phosphorylation
T155 Phosphorylation
S157 Phosphorylation
S160 Phosphorylation
S164 Phosphorylation
S191 Phosphorylation
T217 Phosphorylation
T228 Phosphorylation
S234 Phosphorylation
K335 Ubiquitination
K351 Ubiquitination
K367 Ubiquitination
K377 Ubiquitination
K381 Ubiquitination
K398 Ubiquitination
Y415 Phosphorylation
Y454 Phosphorylation
S455 Phosphorylation
K457 Acetylation
K463 Methylation
K463 Ubiquitination
S493 Phosphorylation
K637 Ubiquitination
K644 Ubiquitination
K650 Ubiquitination
K719 Ubiquitination
T722 Phosphorylation
S726 Phosphorylation
T727 Phosphorylation
S728 Phosphorylation
S736 Phosphorylation
Y835 Phosphorylation
Y904 Phosphorylation
Y905 Phosphorylation
K913 Ubiquitination
S916 Phosphorylation
S965 Phosphorylation
K967 Sumoylation
K968 Sumoylation
S971 Phosphorylation
S980 Phosphorylation
S985 Phosphorylation
S987 Phosphorylation
S995 Phosphorylation
S1004 Phosphorylation
T1008 Phosphorylation
K1017 Ubiquitination
K1037 Ubiquitination
K1038 Ubiquitination
S1039 Phosphorylation
S1060 Phosphorylation
S1062 Phosphorylation
S1063 Phosphorylation
S1067 Phosphorylation
S1068 Phosphorylation
S1069 Phosphorylation
S1070 Phosphorylation
S1076 Phosphorylation
S1099 Phosphorylation
Y1140 Phosphorylation
S1284 Phosphorylation
S1309 Phosphorylation
K1386 Ubiquitination
T1442 Phosphorylation
S1465 Phosphorylation
S1518 Phosphorylation
R1583 Methylation
K1635 Ubiquitination
S1640 Phosphorylation
T1728 Phosphorylation
S1843 Phosphorylation
S1910 Phosphorylation
S1942 Phosphorylation
S1951 Phosphorylation
S1970 Phosphorylation
S1974 Phosphorylation
S1998 Phosphorylation
S2002 Phosphorylation
S2005 Phosphorylation
S2006 Phosphorylation
S2007 Phosphorylation
T2009 Phosphorylation
S2014 Phosphorylation
S2020 Phosphorylation
T2030 Phosphorylation
Y2035 Phosphorylation
S2036 Phosphorylation
S2038 Phosphorylation
Y2039 Phosphorylation
S2041 Phosphorylation
S2043 Phosphorylation
T2045 Phosphorylation
T2050 Phosphorylation

Research Backgrounds

Function:

May link Golgi membranes to the cytoskeleton and participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. Alternatively, in concert with LURAP1 and CDC42BPA/CDC42BPB, has been involved in modulating lamellar actomyosin retrograde flow that is crucial to cell protrusion and migration. May be involved in the maintenance of the stromal cell architectures required for cell to cell contact (By similarity). Regulates trafficking, expression, and activation of innate immune receptors on macrophages. Plays a role to suppress inflammatory responsiveness of macrophages via a mechanism that modulates CD14 trafficking. Acts as a receptor of surfactant-associated protein A (SFTPA1/SP-A) and plays an important role in internalization and clearance of SFTPA1-opsonized S.aureus by alveolar macrophages. Strongly enhances natural killer cell cytotoxicity.

Subcellular Location:

Golgi apparatus. Golgi apparatus>trans-Golgi network. Postsynaptic Golgi apparatus. Cytoplasm>Cytoskeleton>Microtubule organizing center.
Note: Recruited to the Golgi apparatus by GOLPH3 (PubMed:19837035). Localizes to the postsynaptic Golgi apparatus region, also named Golgi outpost, which shapes dendrite morphology by functioning as sites of acentrosomal microtubule nucleation (By similarity).

Endoplasmic reticulum-Golgi intermediate compartment. Cytoplasm>Cytoskeleton.
Note: Colocalizes with actin.

Cytoplasm.
Note: Lacks the PDZ domain (PubMed:12761286). Diffusely localized in the cytoplasm (PubMed:12761286).

Cell surface.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer. Forms a tripartite complex with CDC42BPA/CDC42BPB and LURAP1 with the latter acting as an adapter connecting CDC42BPA/CDC42BPB and MYO18A. Binds F-actin; regulated by ADP and GOLPH3. Interacts with GOLPH3; the interaction is direct and may link Golgi membranes to the actin cytoskeleton. Interacts with JAK3. Interacts with MSR1 and CD14. Isoform 5 interacts with CD11B.

Family&Domains:

The myosin motor domain binds ADP and ATP but has no intrinsic ATPase activity. Mediates ADP-dependent binding to actin (PubMed:23990465).

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family.

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