MDH1 Antibody - #DF12291

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Product: MDH1 Antibody
Catalog: DF12291
Description: Rabbit polyclonal antibody to MDH1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 36 kDa; 36kD(Calculated).
Uniprot: P40925
RRID: AB_2845096

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(92%), Chicken(92%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
MDH1 Antibody detects endogenous levels of total MDH1.
RRID:
AB_2845096
Cite Format: Affinity Biosciences Cat# DF12291, RRID:AB_2845096.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cytoplasmic; Cytosolic malate dehydrogenase; Diiodophenylpyruvate reductase; Malate dehydrogenase 1, NAD (soluble); Malate dehydrogenase; Malate dehydrogenase cytoplasmic; MDH s; mdh1; MDHA; MDHC_HUMAN; MDHs; MGC:1375; MOR2; Soluble malate dehydrogenase;

Immunogens

Immunogen:
Uniprot:

P40925(MDHC_HUMAN) >>Visit HPA database.

Gene(ID):
MDH1(4190)
Sequence:
MSEPIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALPLLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKSVKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFVTTVQQRGAAVIKARKLSSAMSAAKAICDHVRDIWFGTPEGEFVSMGVISDGNSYGVPDDLLYSFPVVIKNKTWKFVEGLPINDFSREKMDLTAKELTEEKESAFEFLSSA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
92
Sheep
92
Dog
92
Chicken
92
Rabbit
92
Xenopus
83
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P40925 As Substrate

Site PTM Type Enzyme
S2 Acetylation
Y22 Phosphorylation
T71 Phosphorylation
K73 Acetylation
K73 Ubiquitination
S89 Phosphorylation
K103 Acetylation
K103 Ubiquitination
K107 Acetylation
K107 Ubiquitination
K110 Methylation
K110 Ubiquitination
S111 Phosphorylation
K118 Acetylation
K118 Ubiquitination
Y119 Phosphorylation
K121 Acetylation
K125 Ubiquitination
C137 S-Nitrosylation
K142 Acetylation
K142 Ubiquitination
K149 Ubiquitination
C154 S-Nitrosylation
K164 Ubiquitination
K170 Ubiquitination
T174 Phosphorylation
K179 Ubiquitination
S188 Phosphorylation
S189 Phosphorylation
T190 Phosphorylation
Y192 Phosphorylation
K199 Ubiquitination
K205 Ubiquitination
Y210 Phosphorylation
K214 Acetylation
K214 Ubiquitination
S217 Phosphorylation
K220 Ubiquitination
R230 Methylation
K236 Ubiquitination
K239 Ubiquitination
S241 Phosphorylation
S242 Phosphorylation
S245 Phosphorylation
T296 Phosphorylation
K298 Acetylation
K298 Ubiquitination
S309 Phosphorylation
K312 Ubiquitination
K318 Ubiquitination
T321 Phosphorylation
K324 Ubiquitination
S326 Phosphorylation
S332 Phosphorylation
S333 Phosphorylation

Research Backgrounds

PTMs:

ISGylated.

Acetylation at Lys-118 dramatically enhances enzymatic activity and promotes adipogenic differentiation.

Subcellular Location:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the LDH/MDH superfamily. MDH type 2 family.

Research Fields

· Metabolism > Carbohydrate metabolism > Citrate cycle (TCA cycle).

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Carbohydrate metabolism > Pyruvate metabolism.

· Metabolism > Carbohydrate metabolism > Glyoxylate and dicarboxylate metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

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