Mast Cell Chymase Antibody - #DF12290

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Product: Mast Cell Chymase Antibody
Catalog: DF12290
Description: Rabbit polyclonal antibody to Mast Cell Chymase
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Dog
Mol.Wt.: 27-30 kDa; 27kD(Calculated).
Uniprot: P23946
RRID: AB_2845095

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Dog(83%)
Clonality:
Polyclonal
Specificity:
Mast Cell Chymase Antibody detects endogenous levels of total Mast Cell Chymase.
RRID:
AB_2845095
Cite Format: Affinity Biosciences Cat# DF12290, RRID:AB_2845095.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Alpha-chymase; Chymase 1; Chymase 1 mast cell; chymase 1 preproprotein transcript E; chymase 1 preproprotein transcript I; Chymase; Chymase, heart; Chymase, mast cell; CMA1; CMA1_HUMAN; CYH; CYM; EC 3.4.21.39; Mast cell chymase 1; Mast cell protease 3; Mast cell protease 5; Mast cell protease I; Mast cell protease III; Mcp-5; MCP3P; Mcpt5; MCT1; MGC119890; MGC119891; MMCP-5;

Immunogens

Immunogen:
Uniprot:

P23946(CMA1_HUMAN) >>Visit HPA database.

Gene(ID):
CMA1(1215)
Expression:
P23946 CMA1_HUMAN:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

Sequence:
MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
83
Rabbit
70
Pig
0
Horse
0
Bovine
0
Sheep
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P23946 As Substrate

Site PTM Type Enzyme
S15 Phosphorylation
Y35 Phosphorylation
Y38 Phosphorylation
N80 N-Glycosylation
N103 N-Glycosylation

Research Backgrounds

Function:

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion.

Subcellular Location:

Secreted. Cytoplasmic granule.
Note: Mast cell granules.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mast cells in lung, heart, skin and placenta. Expressed in both normal skin and in urticaria pigmentosa lesions.

Family&Domains:

Belongs to the peptidase S1 family. Granzyme subfamily.

Research Fields

· Organismal Systems > Endocrine system > Renin-angiotensin system.   (View pathway)

References

1). SYNPO2 promotes the development of BLCA by upregulating the infiltration of resting mast cells and increasing the resistance to immunotherapy. Oncology reports, 2024 (PubMed: 38038167) [IF=4.2]

Application: IF/ICC    Species: Mouse    Sample: lung tissue

Figure 5. SYNPO2 expression enhances mast cell infiltration. (A) SYNPO2 expression in 24 types of immune cell. Red text indicates a high correlation. (B) Representative toluidine and immunohistochemical staining in clinical Bladder Urothelial Carcinoma (BLCA) samples with low and high SYNPO2 expression. Purple represents cell degranulation. Scale bar, 100 µm. (C) Mast cell number/field in low and high SYNPO2 expression groups (P=0.0053). Correlation between SYNPO2 and (D) TPSAB1 and (E) CCR3 expression based on H-score. (F) Mouse serum IL-6 levels in NC (n=5) and SY (n=5; P=0.002). (G) Representative indirect immunofluorescence of a frozen lung section from a mouse model with pulmonary metastasis. The black arrow indicates the area of tumor invasion in the lung tissue. (H) Mouse serum TGF-β1 levels (P=0.045). (I) CMA-positive cells (n=3, P=0.0036). SYNPO2, synaptopodin-2; TPSAB1, Tryptase alpha/beta-1; CCR3, C-C chemokine receptor type 3; NC, Negative Control; SY, SYNPO2 overexpression; CMA1, Chymase 1. * and ** indicate P

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