KIF18A Antibody - #DF12286

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Product: KIF18A Antibody
Catalog: DF12286
Description: Rabbit polyclonal antibody to KIF18A
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 102 kDa; 102kD(Calculated).
Uniprot: Q8NI77
RRID: AB_2845091

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(92%), Sheep(100%), Rabbit(92%), Dog(92%), Chicken(83%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
KIF18A Antibody detects endogenous levels of total KIF18A.
RRID:
AB_2845091
Cite Format: Affinity Biosciences Cat# DF12286, RRID:AB_2845091.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AU024633; B130001M12Rik; DKFZp434G2226; KI18A_HUMAN; KIF 18A; KIF18A; kinesin family member 18A; Kinesin like protein KIF18A; Kinesin-like protein KIF18A; Marrow stromal KIF18A; MGC27627; MS KIF18A; MS-KIF18A; RGD1561740; RP23 206C1.1;

Immunogens

Immunogen:
Uniprot:

Q8NI77(KI18A_HUMAN) >>Visit HPA database.

Gene(ID):
KIF18A(81930)
Sequence:
MSVTEEDLCHHMKVVVRVRPENTKEKAAGFHKVVHVVDKHILVFDPKQEEVSFFHGKKTTNQNVIKKQNKDLKFVFDAVFDETSTQSEVFEHTTKPILRSFLNGYNCTVLAYGATGAGKTHTMLGSADEPGVMYLTMLHLYKCMDEIKEEKICSTAVSYLEVYNEQIRDLLVNSGPLAVREDTQKGVVVHGLTLHQPKSSEEILHLLDNGNKNRTQHPTDMNATSSRSHAVFQIYLRQQDKTASINQNVRIAKMSLIDLAGSERASTSGAKGTRFVEGTNINRSLLALGNVINALADSKRKNQHIPYRNSKLTRLLKDSLGGNCQTIMIAAVSPSSVFYDDTYNTLKYANRAKDIKSSLKSNVLNVNNHITQYVKICNEQKAEILLLKEKLKAYEEQKAFTNENDQAKLMISNPQEKEIERFQEILNCLFQNREEIRQEYLKLEMLLKENELKSFYQQQCHKQIEMMCSEDKVEKATGKRDHRLAMLKTRRSYLEKRREEELKQFDENTNWLHRVEKEMGLLSQNGHIPKELKKDLHCHHLHLQNKDLKAQIRHMMDLACLQEQQHRQTEAVLNALLPTLRKQYCTLKEAGLSNAAFESDFKEIEHLVERKKVVVWADQTAEQPKQNDLPGISVLMTFPQLGPVQPIPCCSSSGGTNLVKIPTEKRTRRKLMPSPLKGQHTLKSPPSQSVQLNDSLSKELQPIVYTPEDCRKAFQNPSTVTLMKPSSFTTSFQAISSNINSDNCLKMLCEVAIPHNRRKECGQEDLDSTFTICEDIKSSKCKLPEQESLPNDNKDILQRLDPSSFSTKHSMPVPSMVPSYMAMTTAAKRKRKLTSSTSNSSLTADVNSGFAKRVRQDNSSEKHLQENKPTMEHKRNICKINPSMVRKFGRNISKGNLR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Xenopus
100
Horse
92
Dog
92
Rabbit
92
Zebrafish
83
Chicken
83
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8NI77 As Substrate

Site PTM Type Enzyme
S2 Phosphorylation
K57 Ubiquitination
K58 Ubiquitination
K241 Ubiquitination
S244 Phosphorylation
K253 Ubiquitination
S284 Phosphorylation
K299 Acetylation
K299 Ubiquitination
K356 Ubiquitination
S357 Phosphorylation
K360 Ubiquitination
K388 Ubiquitination
K398 Sumoylation
K398 Ubiquitination
K408 Ubiquitination
K453 Ubiquitination
K503 Ubiquitination
K517 Methylation
K534 Methylation
K534 Ubiquitination
K546 Ubiquitination
K549 Ubiquitination
K588 Ubiquitination
K602 Ubiquitination
K612 Ubiquitination
S674 Phosphorylation
T681 Phosphorylation
S684 Phosphorylation
S687 Phosphorylation
S695 Phosphorylation
S697 Phosphorylation
T706 Phosphorylation
K712 Ubiquitination
K794 Sumoylation
K794 Ubiquitination
T834 Phosphorylation
S835 Phosphorylation
S838 Phosphorylation
S840 Phosphorylation
S841 Phosphorylation
T843 Phosphorylation
S848 Phosphorylation
K868 Sumoylation
K894 Sumoylation

Research Backgrounds

Function:

Microtubule-depolymerizing kinesin which plays a role in chromosome congression by reducing the amplitude of preanaphase oscillations and slowing poleward movement during anaphase, thus suppressing chromosome movements. May stabilize the CENPE-BUB1B complex at the kinetochores during early mitosis and maintains CENPE levels at kinetochores during chromosome congression.

PTMs:

Glycosylated.

Ubiquitinated.

Subcellular Location:

Cell projection>Ruffle. Cytoplasm. Nucleus. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with CENPE and ESR1.

Family&Domains:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

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