Histone H1.2 Antibody - #DF12279
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12279, RRID:AB_2845084.
Fold/Unfold
H1 histone family member 2; H1.a; H12_HUMAN; H1F2; H1s-1; HIST1H1C; Histone 1 H1c; Histone cluster 1 H1c; Histone H1.2; Histone H1c; Histone H1d; Histone H1s-1; MGC3992;
Immunogens
- P16403 H12_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTGASGSFKLNKKAASGEAKPKVKKAGGTKPKKPVGAAKKPKKAAGGATPKKSAKKTPKKAKKPAAATVTKKVAKSPKKAKVAKPKKAAKSAAKAVKPKAAKPKVVKPKKAAPKKK
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P16403 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S2 | Acetylation | Uniprot | |
| S2 | Phosphorylation | Uniprot | |
| T4 | Phosphorylation | Uniprot | |
| K17 | Acetylation | Uniprot | |
| K17 | Ubiquitination | Uniprot | |
| K21 | Ubiquitination | Uniprot | |
| K26 | Methylation | Uniprot | |
| T31 | Phosphorylation | Uniprot | |
| K34 | Acetylation | Uniprot | |
| K34 | Methylation | Uniprot | |
| K34 | Ubiquitination | Uniprot | |
| S36 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S41 | Phosphorylation | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| K46 | Acetylation | Uniprot | |
| K46 | Ubiquitination | Uniprot | |
| S51 | Phosphorylation | Uniprot | |
| K52 | Acetylation | Uniprot | |
| K52 | Methylation | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| R54 | Methylation | Uniprot | |
| S55 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| S58 | Phosphorylation | Uniprot | |
| K63 | Acetylation | Uniprot | |
| K63 | Ubiquitination | Uniprot | |
| K64 | Sumoylation | Uniprot | |
| K64 | Ubiquitination | Uniprot | |
| Y71 | Phosphorylation | Uniprot | |
| K75 | Acetylation | Uniprot | |
| K75 | Methylation | Uniprot | |
| K75 | Ubiquitination | Uniprot | |
| S78 | Phosphorylation | Uniprot | |
| K85 | Acetylation | Uniprot | |
| K85 | Ubiquitination | Uniprot | |
| S86 | Phosphorylation | Uniprot | |
| S89 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K90 | Acetylation | Uniprot | |
| K90 | Ubiquitination | Uniprot | |
| T92 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K97 | Ubiquitination | Uniprot | |
| T99 | Phosphorylation | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| S104 | Phosphorylation | Uniprot | |
| K106 | Acetylation | Uniprot | |
| K106 | Ubiquitination | Uniprot | |
| K109 | Ubiquitination | Uniprot | |
| K110 | Ubiquitination | Uniprot | |
| S113 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K119 | Methylation | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T126 | Phosphorylation | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K129 | Ubiquitination | Uniprot | |
| K130 | Ubiquitination | Uniprot | |
| K136 | Ubiquitination | Uniprot | |
| K140 | Ubiquitination | Uniprot | |
| T146 | Phosphorylation | P78527 (PRKDC) | Uniprot |
| K148 | Ubiquitination | Uniprot | |
| S150 | Phosphorylation | Uniprot | |
| T154 | Phosphorylation | Uniprot | |
| K157 | Acetylation | Uniprot | |
| K157 | Ubiquitination | Uniprot | |
| K159 | Acetylation | Uniprot | |
| K159 | Ubiquitination | Uniprot | |
| K160 | Acetylation | Uniprot | |
| K160 | Ubiquitination | Uniprot | |
| T165 | Phosphorylation | Uniprot | |
| T167 | Phosphorylation | O96017 (CHEK2) | Uniprot |
| K168 | Acetylation | Uniprot | |
| K168 | Methylation | Uniprot | |
| K168 | Ubiquitination | Uniprot | |
| K169 | Acetylation | Uniprot | |
| K169 | Ubiquitination | Uniprot | |
| S173 | Phosphorylation | Uniprot | |
| K178 | Ubiquitination | Uniprot | |
| K187 | Acetylation | Uniprot | |
| K187 | Methylation | Uniprot | |
| S188 | Phosphorylation | Uniprot | |
| K191 | Ubiquitination | Uniprot | |
| K194 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| K199 | Ubiquitination | Uniprot | |
| K201 | Ubiquitination | Uniprot | |
| K206 | Ubiquitination | Uniprot |
Research Backgrounds
Histone H1 protein binds to linker DNA between nucleosomes forming the macromolecular structure known as the chromatin fiber. Histones H1 are necessary for the condensation of nucleosome chains into higher-order structured fibers. Acts also as a regulator of individual gene transcription through chromatin remodeling, nucleosome spacing and DNA methylation (By similarity).
H1 histones are progressively phosphorylated during the cell cycle, becoming maximally phosphorylated during late G2 phase and M phase, and being dephosphorylated sharply thereafter.
Crotonylation (Kcr) is specifically present in male germ cells and marks testis-specific genes in post-meiotic cells, including X-linked genes that escape sex chromosome inactivation in haploid cells. Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors. It is also associated with post-meiotically activated genes on autosomes.
Citrullination at Arg-54 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.
ADP-ribosylated on Ser-188 in response to DNA damage.
Nucleus. Chromosome.
Note: Mainly localizes in euchromatin. Distribution goes in parallel with DNA concentration.
The C-terminal domain is required for high-affinity binding to chromatin.
Belongs to the histone H1/H5 family.
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