Product: Hemoglobin epsilon Antibody
Catalog: DF12277
Description: Rabbit polyclonal antibody to Hemoglobin epsilon
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Rabbit
Mol.Wt.: 16 kDa; 16kD(Calculated).
Uniprot: P02100
RRID: AB_2845082

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Bovine(82%), Rabbit(100%)
Clonality:
Polyclonal
Specificity:
Hemoglobin epsilon Antibody detects endogenous levels of total Hemoglobin epsilon.
RRID:
AB_2845082
Cite Format: Affinity Biosciences Cat# DF12277, RRID:AB_2845082.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Epsilon-globin; HBE_HUMAN; HBE1; Hemoglobin epsilon chain; Hemoglobin subunit epsilon;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P02100 HBE_HUMAN:

Red blood cells.

Sequence:
MVHFTAEEKAAVTSLWSKMNVEEAGGEALGRLLVVYPWTQRFFDSFGNLSSPSAILGNPKVKAHGKKVLTSFGDAIKNMDNLKPAFAKLSELHCDKLHVDPENFKLLGNVMVIILATHFGKEFTPEVQAAWQKLVSAVAIALAHKYH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Bovine
82
Chicken
64
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P02100 As Substrate

Site PTM Type Enzyme
S14 Phosphorylation
S17 Phosphorylation
K18 Ubiquitination
Y36 Phosphorylation
T39 Phosphorylation
S50 Phosphorylation
S51 Phosphorylation
K60 Acetylation
K60 Ubiquitination
K66 Acetylation
K67 Ubiquitination
S71 Phosphorylation
K77 Acetylation
K77 Ubiquitination
K83 Acetylation
K83 Ubiquitination
K145 Acetylation

Research Backgrounds

Function:

The epsilon chain is a beta-type chain of early mammalian embryonic hemoglobin.

Tissue Specificity:

Red blood cells.

Subunit Structure:

Heterotetramer of two alpha chains and two epsilon chains in early embryonic hemoglobin Gower-2; two zeta chains and two epsilon chains in early embryonic hemoglobin Gower-1.

Family&Domains:

Belongs to the globin family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.