DNAJC10 Antibody - #DF12269
| Product: | DNAJC10 Antibody |
| Catalog: | DF12269 |
| Description: | Rabbit polyclonal antibody to DNAJC10 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus |
| Mol.Wt.: | 75-80 kDa; 91kD(Calculated). |
| Uniprot: | Q8IXB1 |
| RRID: | AB_2845074 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12269, RRID:AB_2845074.
Fold/Unfold
DKFZp434J1813; DnaJ (Hsp40) homolog subfamily C member 10; DnaJ homolog subfamily C member 10; DNAJC 10; ER resident protein ERdj5; ERdj 5; ERdj5; J domain containing protein disulfide isomerase like protein; JPDI; Macrothioredoxin; MGC104194; MTHr;
Immunogens
- Q8IXB1 DJC10_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGVWLNKDDYIRDLKRIILCFLIVYMAILVGTDQDFYSLLGVSKTASSREIRQAFKKLALKLHPDKNPNNPNAHGDFLKINRAYEVLKDEDLRKKYDKYGEKGLEDNQGGQYESWNYYRYDFGIYDDDPEIITLERREFDAAVNSGELWFVNFYSPGCSHCHDLAPTWRDFAKEVDGLLRIGAVNCGDDRMLCRMKGVNSYPSLFIFRSGMAPVKYHGDRSKESLVSFAMQHVRSTVTELWTGNFVNSIQTAFAAGIGWLITFCSKGGDCLTSQTRLRLSGMLDGLVNVGWMDCATQDNLCKSLDITTSTTAYFPPGATLNNKEKNSILFLNSLDAKEIYLEVIHNLPDFELLSANTLEDRLAHHRWLLFFHFGKNENSNDPELKKLKTLLKNDHIQVGRFDCSSAPDICSNLYVFQPSLAVFKGQGTKEYEIHHGKKILYDILAFAKESVNSHVTTLGPQNFPANDKEPWLVDFFAPWCPPCRALLPELRRASNLLYGQLKFGTLDCTVHEGLCNMYNIQAYPTTVVFNQSNIHEYEGHHSAEQILEFIEDLMNPSVVSLTPTTFNELVTQRKHNEVWMVDFYSPWCHPCQVLMPEWKRMARTLTGLINVGSIDCQQYHSFCAQENVQRYPEIRFFPPKSNKAYHYHSYNGWNRDAYSLRIWGLGFLPQVSTDLTPQTFSEKVLQGKNHWVIDFYAPWCGPCQNFAPEFELLARMIKGKVKAGKVDCQAYAQTCQKAGIRAYPTVKFYFYERAKRNFQEEQINTRDAKAIAALISEKLETLRNQGKRNKDEL
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8IXB1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K66 | Ubiquitination | Uniprot | |
| Y112 | Phosphorylation | Uniprot | |
| T133 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K196 | Ubiquitination | Uniprot | |
| S327 | Phosphorylation | Uniprot | |
| K392 | Ubiquitination | Uniprot | |
| K438 | Ubiquitination | Uniprot | |
| K640 | Acetylation | Uniprot | |
| S672 | Phosphorylation | Uniprot | |
| T673 | Phosphorylation | Uniprot | |
| S681 | Phosphorylation | Uniprot | |
| K725 | Ubiquitination | Uniprot | |
| K737 | Ubiquitination | Uniprot | |
| K769 | Ubiquitination | Uniprot | |
| K778 | Ubiquitination | Uniprot | |
| R783 | Methylation | Uniprot |
Research Backgrounds
Endoplasmic reticulum disulfide reductase involved both in the correct folding of proteins and degradation of misfolded proteins. Required for efficient folding of proteins in the endoplasmic reticulum by catalyzing the removal of non-native disulfide bonds formed during the folding of proteins, such as LDLR. Also involved in endoplasmic reticulum-associated degradation (ERAD) by reducing incorrect disulfide bonds in misfolded glycoproteins recognized by EDEM1. Interaction with HSPA5 is required its activity, not for the disulfide reductase activity, but to facilitate the release of DNAJC10 from its substrate. Promotes apoptotic signaling pathway in response to endoplasmic reticulum stress.
Endoplasmic reticulum lumen.
Interacts with EDEM1 (By similarity). Interacts with HSPA5 (via its J domain).
The thioredoxin-like regions Trxb 1 and 2 lack a redox-active CXXC motif.
Thioredoxin domains 3 and 4 are the primary reductase domains.
Research Fields
· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum. (View pathway)
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