Product: DHX38 Antibody
Catalog: DF12265
Description: Rabbit polyclonal antibody to DHX38
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 141 kDa; 141kD(Calculated).
Uniprot: Q92620
RRID: AB_2845070

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(92%)
Clonality:
Polyclonal
Specificity:
DHX38 Antibody detects endogenous levels of total DHX38.
RRID:
AB_2845070
Cite Format: Affinity Biosciences Cat# DF12265, RRID:AB_2845070.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ATP dependent RNA helicase DHX38; ATP-dependent RNA helicase DHX38; DDX38; DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38; DEAH (Asp-Glu-Ala-His) box polypeptide 38; DEAH box protein 38; DHX38; KIAA0224; Pre mRNA splicing factor ATP dependent RNA helicase PRP16; Pre-mRNA splicing factor 16; Pre-mRNA-splicing factor ATP-dependent RNA helicase prp16; Prp16; PRP16 homolog of S.cerevisiae; pre-mRNA splicing factor ATP-dependent RNA helicase PRP16; PRP16_HUMAN; PRPF16;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Sequence:
MGDTSEDASIHRLEGTDLDCQVGGLICKSKSAASEQHVFKAPAPRPSLLGLDLLASLKRREREEKDDGEDKKKSKVSSYKDWEESKDDQKDAEEEGGDQAGQNIRKDRHYRSARVETPSHPGGVSEEFWERSRQRERERREHGVYASSKEEKDWKKEKSRDRDYDRKRDRDERDRSRHSSRSERDGGSERSSRRNEPESPRHRPKDAATPSRSTWEEEDSGYGSSRRSQWESPSPTPSYRDSERSHRLSTRDRDRSVRGKYSDDTPLPTPSYKYNEWADDRRHLGSTPRLSRGRGRREEGEEGISFDTEEERQQWEDDQRQADRDWYMMDEGYDEFHNPLAYSSEDYVRRREQHLHKQKQKRISAQRRQINEDNERWETNRMLTSGVVHRLEVDEDFEEDNAAKVHLMVHNLVPPFLDGRIVFTKQPEPVIPVKDATSDLAIIARKGSQTVRKHREQKERKKAQHKHWELAGTKLGDIMGVKKEEEPDKAVTEDGKVDYRTEQKFADHMKRKSEASSEFAKKKSILEQRQYLPIFAVQQELLTIIRDNSIVIVVGETGSGKTTQLTQYLHEDGYTDYGMIGCTQPRRVAAMSVAKRVSEEMGGNLGEEVGYAIRFEDCTSENTLIKYMTDGILLRESLREADLDHYSAIIMDEAHERSLNTDVLFGLLREVVARRSDLKLIVTSATMDAEKFAAFFGNVPIFHIPGRTFPVDILFSKTPQEDYVEAAVKQSLQVHLSGAPGDILIFMPGQEDIEVTSDQIVEHLEELENAPALAVLPIYSQLPSDLQAKIFQKAPDGVRKCIVATNIAETSLTVDGIMFVIDSGYCKLKVFNPRIGMDALQIYPISQANANQRSGRAGRTGPGQCFRLYTQSAYKNELLTTTVPEIQRTNLANVVLLLKSLGVQDLLQFHFMDPPPEDNMLNSMYQLWILGALDNTGGLTSTGRLMVEFPLDPALSKMLIVSCDMGCSSEILLIVSMLSVPAIFYRPKGREEESDQIREKFAVPESDHLTYLNVYLQWKNNNYSTIWCNDHFIHAKAMRKVREVRAQLKDIMVQQRMSLASCGTDWDIVRKCICAAYFHQAAKLKGIGEYVNIRTGMPCHLHPTSSLFGMGYTPDYIVYHELVMTTKEYMQCVTAVDGEWLAELGPMFYSVKQAGKSRQENRRRAKEEASAMEEEMALAEEQLRARRQEQEKRSPLGSVRSTKIYTPGRKEQGEPMTPRRTPARFGL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
92
Xenopus
75
Zebrafish
75
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q92620 As Substrate

Site PTM Type Enzyme
G2 Acetylation
S47 Phosphorylation
S56 Phosphorylation
K58 Acetylation
T117 Phosphorylation
Y145 Phosphorylation
K149 Sumoylation
S180 Phosphorylation
S199 Phosphorylation
S224 Phosphorylation
S232 Phosphorylation P24941 (CDK2)
S234 Phosphorylation P24941 (CDK2)
T236 Phosphorylation
S242 Phosphorylation
K260 Acetylation
K260 Methylation
T265 Phosphorylation
T269 Phosphorylation
Y272 Phosphorylation
T287 Phosphorylation
R289 Methylation
S291 Phosphorylation
T384 Phosphorylation
K425 Sumoylation
K425 Ubiquitination
K434 Ubiquitination
S448 Phosphorylation
K466 Ubiquitination
K482 Sumoylation
K504 Sumoylation
K504 Ubiquitination
K510 Ubiquitination
S513 Phosphorylation
K521 Acetylation
K595 Ubiquitination
K679 Acetylation
T708 Phosphorylation
K793 Ubiquitination
T805 Phosphorylation
K829 Ubiquitination
T860 Phosphorylation
K875 Ubiquitination
K1049 Ubiquitination
S1058 Phosphorylation
T1064 Phosphorylation
K1083 Acetylation
K1166 Sumoylation
K1166 Ubiquitination
S1194 Phosphorylation
S1198 Phosphorylation
T1206 Phosphorylation
T1217 Phosphorylation
T1221 Phosphorylation

Research Backgrounds

Function:

Probable ATP-binding RNA helicase (Probable). Involved in pre-mRNA splicing as component of the spliceosome.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in the spliceosome C complex.

Family&Domains:

Belongs to the DEAD box helicase family. DEAH subfamily. PRP16 sub-subfamily.

Research Fields

· Genetic Information Processing > Transcription > Spliceosome.

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