CPSF4 Antibody - #DF12260

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Product: CPSF4 Antibody
Catalog: DF12260
Description: Rabbit polyclonal antibody to CPSF4
Application: WB IHC
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 30 kDa; 30kD(Calculated).
Uniprot: O95639
RRID: AB_2845065

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 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(92%), Dog(100%)
Clonality:
Polyclonal
Specificity:
CPSF4 Antibody detects endogenous levels of total CPSF4.
RRID:
AB_2845065
Cite Format: Affinity Biosciences Cat# DF12260, RRID:AB_2845065.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cleavage and polyadenylation specific factor 4, 30kDa; Cleavage and polyadenylation specificity factor 30 kDa subunit; Cleavage and polyadenylation specificity factor subunit 4; cleavage polyadenylation specificity factor, 30kD; CPSF 30 kDa subunit; CPSF30; Cpsf4; CPSF4_HUMAN; NAR; Neb-1; NEB1; No arches homolog; no arches like zinc finger protein; NS1 effector domain binding protein 1; NS1 effector domain-binding protein 1; OTTHUMP00000206235; OTTHUMP00000206237; OTTHUMP00000206239;

Immunogens

Immunogen:
Uniprot:

O95639(CPSF4_HUMAN) >>Visit HPA database.

Gene(ID):
CPSF4(10898)
Sequence:
MQEIIASVDHIKFDLEIAVEQQLGAQPLPFPGMDKSGAAVCEFFLKAACGKGGMCPFRHISGEKTVVCKHWLRGLCKKGDQCEFLHEYDMTKMPECYFYSKFGECSNKECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYLVGFCPEGPSCKFMHPRFELPMGTTEQPPLPQQTQPPAKQSNNPPLQRSSSLIQLTSQNSSPNQQRTPQVIGVMQSQNSSAGNRGPRPLEQVTCYKCGEKGHYANRCTKGHLAFLSGQ

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Bovine
92
Sheep
92
Rabbit
92
Chicken
75
Xenopus
64
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O95639 As Substrate

Site PTM Type Enzyme
S7 Phosphorylation
K46 Methylation
K46 Ubiquitination
K51 Methylation
K51 Ubiquitination
S61 Phosphorylation
K69 Ubiquitination
Y97 Phosphorylation
K108 Ubiquitination
K120 Ubiquitination
K122 Ubiquitination
K133 Acetylation
K133 Ubiquitination
S200 Phosphorylation
S201 Phosphorylation
S202 Phosphorylation
S208 Phosphorylation
S211 Phosphorylation
S212 Phosphorylation
S231 Phosphorylation
S267 Phosphorylation

Research Backgrounds

Function:

Component of the cleavage and polyadenylation specificity factor (CPSF) complex that play a key role in pre-mRNA 3'-end formation, recognizing the AAUAAA signal sequence and interacting with poly(A) polymerase and other factors to bring about cleavage and poly(A) addition. CPSF4 binds RNA polymers with a preference for poly(U).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Component of the cleavage and polyadenylation specificity factor (CPSF) complex, composed of CPSF1, CPSF2, CPSF3, CPSF4 and FIP1L1. Interacts with FIP1L1.

(Microbial infection) Interacts with influenza A virus NS1 blocks processing of pre-mRNAs, thereby preventing nuclear export of host cell mRNAs.

Family&Domains:

Belongs to the CPSF4/YTH1 family.

Research Fields

· Genetic Information Processing > Translation > mRNA surveillance pathway.

· Human Diseases > Infectious diseases: Viral > Influenza A.

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