CHCHD6 Antibody | Affinity Biosciences

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Western blot analysis of extracts from rat brain, using CHCHD6 antibody.

Product:	CHCHD6 Antibody
Catalog:	DF12255
Description:	Rabbit polyclonal antibody to CHCHD6
Application:	WB IHC
Reactivity:	Human, Rat
Prediction:	Pig, Bovine, Rabbit
Mol.Wt.:	26-29 kDa; 26kD(Calculated).
Uniprot:	Q9BRQ6
RRID:	AB_2845060

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Rat

Prediction:

Pig(89%), Bovine(100%), Rabbit(100%)

Clonality:

Polyclonal

Specificity:

CHCHD6 Antibody detects endogenous levels of total CHCHD6.

RRID:

AB_2845060
Cite Format: Affinity Biosciences Cat# DF12255, RRID:AB_2845060.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CHCH6_HUMAN; Chchd6; CHCM1; Coiled coil helix cristae morphology 1; Coiled coil helix cristae morphology protein 1; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6; Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial; Mic25; MICOS complex subunit MIC25; PPP1R23; Protein phosphatase 1 regulatory subunit 23;

Immunogens

Immunogen:

Uniprot:

Q9BRQ6(MIC25_HUMAN) >>Visit HPA database.

Gene(ID):

CHCHD6(84303)

Sequence:

Q9BRQ6 MIC25_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGSTESSEGRRVSFGVDEEERVRVLQGVRLSENVVNRMKEPSSPPPAPTSSTFGLQDGNLRAPHKESTLPRSGSSGGQQPSGMKEGVKRYEQEHAAIQDKLFQVAKREREAATKHSKASLPTGEGSISHEEQKSVRLARELESREAELRRRDTFYKEQLERIERKNAEMYKLSSEQFHEAASKMESTIKPRRVEPVCSGLQAQILHCYRDRPHEVLLCSDLVKAYQRCVSAAHKG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

100

Bovine

100

Pig

Horse

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BRQ6 As Substrate

Q9BRQ6

Site	PTM Type	Source
S13	Phosphorylation	Uniprot
S42	Phosphorylation	Uniprot
S43	Phosphorylation	Uniprot
T49	Phosphorylation	Uniprot
K65	Ubiquitination	Uniprot
S67	Phosphorylation	Uniprot
S74	Phosphorylation	Uniprot
K84	Acetylation	Uniprot
K100	Acetylation	Uniprot
K106	Acetylation	Uniprot
K114	Acetylation	Uniprot
K114	Ubiquitination	Uniprot
K117	Acetylation	Uniprot
K133	Acetylation	Uniprot
S134	Phosphorylation	Uniprot
T153	Phosphorylation	Uniprot
Y155	Phosphorylation	Uniprot
K156	Ubiquitination	Uniprot
Y170	Phosphorylation	Uniprot
S173	Phosphorylation	Uniprot
S186	Phosphorylation	Uniprot
T187	Phosphorylation	Uniprot

Research Backgrounds

Q9BRQ6_MIC25_HUMAN

Function:

Component of the MICOS complex, a large protein complex of the mitochondrial inner membrane that plays crucial roles in the maintenance of crista junctions, inner membrane architecture, and formation of contact sites to the outer membrane.

Subcellular Location:

Mitochondrion inner membrane>Lipid-anchor. Mitochondrion.

Subunit Structure:

Component of the mitochondrial contact site and cristae organizing system (MICOS) complex, composed of at least MICOS10/MIC10, CHCHD3/MIC19, CHCHD6/MIC25, APOOL/MIC27, IMMT/MIC60, APOO/MIC23/MIC26 and MICOS13/MIC13. This complex was also known under the names MINOS or MitOS complex. The MICOS complex associates with mitochondrial outer membrane proteins SAMM50, MTX1 and MTX2 (together described as components of the mitochondrial outer membrane sorting assembly machinery (SAM) complex) and DNAJC11, mitochondrial inner membrane protein TMEM11 and with HSPA9. The MICOS and SAM complexes together with DNAJC11 are part of a large protein complex spanning both membranes termed the mitochondrial intermembrane space bridging (MIB) complex. Interacts with DISC1. Interacts with DISC1. Interacts with IMMT/MIC60.

Family&Domains:

Belongs to the MICOS complex subunit Mic19 family. Metazoan Mic25 subfamily.

Restrictive clause

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CHCHD6 Antibody - #DF12255