Centrin 2 Antibody - #DF12252
| Product: | Centrin 2 Antibody |
| Catalog: | DF12252 |
| Description: | Rabbit polyclonal antibody to Centrin 2 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 21 kDa; 20kD(Calculated). |
| Uniprot: | P41208 |
| RRID: | AB_2845057 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF12252, RRID:AB_2845057.
Fold/Unfold
20kD calcium binding protein; CALT; caltractin; Caltractin isoform 1; CEN2; centrin; centrin, EF hand protein, 2; Centrin-2; Centrin2; CETN2; CETN2_HUMAN; EF hand protein 2; EF-hand protein;
Immunogens
- P41208 CETN2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MASNFKKANMASSSQRKRMSPKPELTEEQKQEIREAFDLFDADGTGTIDVKELKVAMRALGFEPKKEEIKKMISEIDKEGTGKMNFGDFLTVMTQKMSEKDTKEEILKAFKLFDDDETGKISFKNLKRVAKELGENLTDEELQEMIDEADRDGDGEVSEQEFLRIMKKTSLY
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P41208 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| Sumoylation | Uniprot | ||
| A2 | Acetylation | Uniprot | |
| S3 | Phosphorylation | Uniprot | |
| K6 | Acetylation | Uniprot | |
| K6 | Methylation | Uniprot | |
| K7 | Methylation | Uniprot | |
| S13 | Phosphorylation | Uniprot | |
| S14 | Phosphorylation | Uniprot | |
| S20 | Phosphorylation | Uniprot | |
| K22 | Ubiquitination | Uniprot | |
| T26 | Phosphorylation | Uniprot | |
| K30 | Ubiquitination | Uniprot | |
| T45 | Phosphorylation | Uniprot | |
| T47 | Phosphorylation | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| K65 | Ubiquitination | Uniprot | |
| K78 | Ubiquitination | Uniprot | |
| K103 | Ubiquitination | Uniprot | |
| K108 | Ubiquitination | Uniprot | |
| K111 | Ubiquitination | Uniprot | |
| T118 | Phosphorylation | Uniprot | |
| K120 | Ubiquitination | Uniprot | |
| S122 | Phosphorylation | O14965 (AURKA) | Uniprot |
| K124 | Ubiquitination | Uniprot | |
| K127 | Ubiquitination | Uniprot | |
| K131 | Ubiquitination | Uniprot | |
| T138 | Phosphorylation | Uniprot | |
| S158 | Phosphorylation | Uniprot | |
| K167 | Ubiquitination | Uniprot | |
| S170 | Phosphorylation | O14965 (AURKA) , P17612 (PRKACA) | Uniprot |
| Y172 | Phosphorylation | Uniprot |
Research Backgrounds
Plays a fundamental role in microtubule organizing center structure and function. Required for centriole duplication and correct spindle formation. Has a role in regulating cytokinesis and genome stability via cooperation with CALM1 and CCP110.
Involved in global genome nucleotide excision repair (GG-NER) by acting as component of the XPC complex. Cooperatively with RAD23B appears to stabilize XPC. In vitro, stimulates DNA binding of the XPC:RAD23B dimer.
The XPC complex is proposed to represent the first factor bound at the sites of DNA damage and together with other core recognition factors, XPA, RPA and the TFIIH complex, is part of the pre-incision (or initial recognition) complex. The XPC complex recognizes a wide spectrum of damaged DNA characterized by distortions of the DNA helix such as single-stranded loops, mismatched bubbles or single-stranded overhangs. The orientation of XPC complex binding appears to be crucial for inducing a productive NER. XPC complex is proposed to recognize and to interact with unpaired bases on the undamaged DNA strand which is followed by recruitment of the TFIIH complex and subsequent scanning for lesions in the opposite strand in a 5'-to-3' direction by the NER machinery. Cyclobutane pyrimidine dimers (CPDs) which are formed upon UV-induced DNA damage esacpe detection by the XPC complex due to a low degree of structural perurbation. Instead they are detected by the UV-DDB complex which in turn recruits and cooperates with the XPC complex in the respective DNA repair.
As a component of the TREX-2 complex, involved in the export of mRNAs to the cytoplasm through the nuclear pores.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome>Centriole. Nucleus envelope. Nucleus>Nuclear pore complex. Nucleus.
Monomer. Homooligomer. Interacts with SFI1. Interacts with CCP110. Component of the XPC complex composed of XPC, RAD23B and CETN2. Component of the nuclear pore complex (NPC)-associated TREX-2 complex (transcription and export complex 2), composed of at least GANP, 2 copies of ENY2, PCID2, SEM1/DSS1, and either centrin CETN2 or centrin CETN3. The TREX-2 complex also associates with ALYREF/ALY and with the nucleoporin NUP153.
Belongs to the centrin family.
Research Fields
· Genetic Information Processing > Replication and repair > Nucleotide excision repair.
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