ACADVL Antibody | Affinity Biosciences

WB
IF/ICC

1/3

Western blot analysis of extracts from mouse brain, using ACADVL antibody.

Western blot analysis of extracts from Colo205, using ACADVL Antibody.

Product:	ACADVL Antibody
Catalog:	DF12244
Description:	Rabbit polyclonal antibody to ACADVL
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Bovine, Sheep, Rabbit, Dog
Mol.Wt.:	98 kDa,51 kDa; 70kD(Calculated).
Uniprot:	P49748
RRID:	AB_2845049

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Bovine(91%), Sheep(91%), Rabbit(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

ACADVL Antibody detects endogenous levels of total ACADVL.

RRID:

AB_2845049
Cite Format: Affinity Biosciences Cat# DF12244, RRID:AB_2845049.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

ACAD 6; ACAD6; ACADV_HUMAN; Acadvl; Acyl CoA dehydrogenase very long chain; Acyl Coenzyme A dehydrogenase very long chain; LCACD; mitochondrial; Very long chain specific acyl CoA dehydrogenase; Very long chain specific acyl CoA dehydrogenase mitochondrial; Very long-chain specific acyl-CoA dehydrogenase; VLCAD;

Immunogens

Immunogen:

Uniprot:

P49748(ACADV_HUMAN) >>Visit HPA database.

Gene(ID):

ACADVL(37)

Sequence:

P49748 ACADV_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQAARMAASLGRQLLRLGGGSSRLTALLGQPRPGPARRPYAGGAAQLALDKSDSHPSDALTRKKPAKAESKSFAVGMFKGQLTTDQVFPYPSVLNEEQTQFLKELVEPVSRFFEEVNDPAKNDALEMVEETTWQGLKELGAFGLQVPSELGGVGLCNTQYARLVEIVGMHDLGVGITLGAHQSIGFKGILLFGTKAQKEKYLPKLASGETVAAFCLTEPSSGSDAASIRTSAVPSPCGKYYTLNGSKLWISNGGLADIFTVFAKTPVTDPATGAVKEKITAFVVERGFGGITHGPPEKKMGIKASNTAEVFFDGVRVPSENVLGEVGSGFKVAMHILNNGRFGMAAALAGTMRGIIAKAVDHATNRTQFGEKIHNFGLIQEKLARMVMLQYVTESMAYMVSANMDQGATDFQIEAAISKIFGSEAAWKVTDECIQIMGGMGFMKEPGVERVLRDLRIFRIFEGTNDILRLFVALQGCMDKGKELSGLGSALKNPFGNAGLLLGEAGKQLRRRAGLGSGLSLSGLVHPELSRSGELAVRALEQFATVVEAKLIKHKKGIVNEQFLLQRLADGAIDLYAMVVVLSRASRSLSEGHPTAQHEKMLCDTWCIEAAARIREGMAALQSDPWQQELYRNFKSISKALVERGGVVTSNPLGF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Bovine

Sheep

Dog

Rabbit

Xenopus

Zebrafish

Pig

Horse

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P49748 As Substrate

P49748

Site	PTM Type	Source
S54	Phosphorylation	Uniprot
K71	Acetylation	Uniprot
K239	Acetylation	Uniprot
K247	Methylation	Uniprot
K276	Acetylation	Uniprot
K278	Acetylation	Uniprot
R286	Methylation	Uniprot
K298	Acetylation	Uniprot
K299	Acetylation	Uniprot
S328	Phosphorylation	Uniprot
K331	Acetylation	Uniprot
K372	Acetylation	Uniprot
K372	Ubiquitination	Uniprot
K482	Acetylation	Uniprot
S485	Phosphorylation	Uniprot
S489	Phosphorylation	Uniprot
K492	Ubiquitination	Uniprot
K507	Acetylation	Uniprot
K507	Ubiquitination	Uniprot
S517	Phosphorylation	Uniprot
S522	Phosphorylation	Uniprot
S586	Phosphorylation	Uniprot
Y631	Phosphorylation	Uniprot
K635	Sumoylation	Uniprot
K639	Acetylation	Uniprot
K639	Ubiquitination	Uniprot
R644	Methylation	Uniprot

Research Backgrounds

P49748_ACADV_HUMAN

Function:

Active toward esters of long-chain and very long chain fatty acids such as palmitoyl-CoA, myristoyl-CoA and stearoyl-CoA. Can accommodate substrate acyl chain lengths as long as 24 carbons, but shows little activity for substrates of less than 12 carbons.

PTMs:

S-nitrosylation at Cys-237 in liver improves catalytic efficiency.

Subcellular Location:

Mitochondrion inner membrane.

Subunit Structure:

Homodimer.

Family&Domains:

Belongs to the acyl-CoA dehydrogenase family.

Research Fields

· Metabolism > Lipid metabolism > Fatty acid degradation.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Fatty acid metabolism.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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ACADVL Antibody - #DF12244