SYVN1 Antibody - #DF12235

(1)   (16)  
Product: SYVN1 Antibody
Catalog: DF12235
Description: Rabbit polyclonal antibody to SYVN1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 68-76 kDa; 68kD(Calculated).
Uniprot: Q86TM6
RRID: AB_2845040

View similar products>>

   Size Price Inventory
 100ul $280 In stock
 200ul $350 In stock

Lead Time: Same day delivery

For pricing and ordering contact:
Local distributors
Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
SYVN1 Antibody detects endogenous levels of total SYVN1.
RRID:
AB_2845040
Cite Format: Affinity Biosciences Cat# DF12235, RRID:AB_2845040.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

1200010C09Rik; DER3; E3 ubiquitin-protein ligase synoviolin; HMG coA reductase degradation 1 homolog; HRD1; KIAA1810; MGC40372; OTTHUMP00000230429; OTTHUMP00000230430; OTTHUMP00000230431; OTTHUMP00000230432; Synovial apoptosis inhibitor 1; Synovial apoptosis inhibitor 1, synoviolin; Synoviolin 1 isoform b; SYNOVIOLIN; SYVN1; SYVN1_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q86TM6(SYVN1_HUMAN) >>Visit HPA database.

Gene(ID):
SYVN1(84447)
Expression:
Q86TM6 SYVN1_HUMAN:

Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).

Sequence:
MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLRAAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCRIVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAVYMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDATPEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPADQGPPPAPHPPPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAVAPPSTSAAALSRPSGAATTTAAGTSATAASATASGPGSGSAPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHERQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNSTEETATTVVAAASSTSIPSSEATTPTPGASPPAPEMERPPAPESVGTEEMPEDGEPDAAELRRRRLQKLESPVAH

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
67
Zebrafish
67
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86TM6 As Substrate

Site PTM Type Enzyme
T93 Phosphorylation
K191 Ubiquitination
K257 Ubiquitination
T260 Phosphorylation
S265 Phosphorylation
K303 Ubiquitination
T557 Phosphorylation
S558 Phosphorylation
S562 Phosphorylation
T565 Phosphorylation
S572 Phosphorylation
S613 Phosphorylation

Research Backgrounds

Function:

Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation. Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins. Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Protects neurons from apoptosis induced by polyglutamine-expanded huntingtin (HTT) or unfolded GPR37 by promoting their degradation. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis. Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (By similarity).

PTMs:

Not N-glycosylated.

Auto-ubiquitinated.

Subcellular Location:

Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed, with highest levels in liver and kidney (at protein level). Up-regulated in synovial tissues from patients with rheumatoid arthritis (at protein level).

Subunit Structure:

Homodimer. Interacts with p53/TP53 and HTT. Interacts with VCP, HERPUD1 and DERL1. Part of a complex containing SYVN1, HERPUD1, VIMP and DERL1; which probably transfer misfolded proteins from the ER to VCP. Part of a complex containing SYVN1, SEL1L and DERL2. Interacts with UBXN6. Interacts (via N-terminal loop) with SEL1L; recruits ERLEC1 and OS9. May form a complex with ERLEC1; HSPA5; OS9 AND SEL1L. Interacts with BAG6. Interacts with NFE2L1 (By similarity).

Family&Domains:

The RING-type zinc finger is required for E3 ligase activity.

Belongs to the HRD1 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

References

1). Endoplasmic reticulum stress-triggered ferroptosis via the XBP1-Hrd1-Nrf2 pathway induces EMT progression in diabetic nephropathy.. Biomedicine & Pharmacotherapy, 2023 (PubMed: 37224754) [IF=7.5]

Application: IHC    Species: Mouse    Sample: kidney tissues

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.

Application: WB    Species: Human    Sample: HK-2 cells

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.

Application: IF/ICC    Species: Human    Sample: HK-2 cells

Fig. 6. The XBP1-Hrd1 arm of the ERS pathway was triggered by high glucose. The XBP1s and Hrd1 expression in tissue samples was detected by immunohistochemical staining (n = 3) (A). Semi-quantitative analysis of XBP1s and Hrd1 expression in each group (n = 3) (B). The qRT-PCR results of Hrd1 in NC, DM, TUDCA, and PBS group (n = 3) (C). The protein expression of Hrd1 in each group of mice (n = 3) (D - E). The fluorescence intensity of XBP1s and Hrd1 in HK-2 cells was measured by the confocal fluorescence microscope. Blue represented the cell nucleus and red represented XBP1s or Hrd1 (n = 3) (F - G). Fluorescence intensity was quantified using Image J (n = 3) (H - I). The mRNA level of Hrd1 among Ctrl, HG, Mannitol, TUDCA, and DMSO groups (n = 3) (J). Immunoblot and semi-quantitative analysis of Hrd1 in each group cells (n = 3) (K - L). The qRT-PCR results of XBP1s and Hrd1 in cells transfected with empty vectors or XBP1 overexpression plasmids. At 6 h post-transfection, cells were changed to a medium containing 30 mmol/L glucose with or without TUDCA (200 μM) for 48 h (n = 3) (M). Immunoblot and quantitative analysis of XBP1s and Hrd1 under the same conditions (n = 3) (N - O). Data are expressed as the mean ± SEM.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.