SRP68 Antibody - #DF12232

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Product: SRP68 Antibody
Catalog: DF12232
Description: Rabbit polyclonal antibody to SRP68
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 68 kDa; 71kD(Calculated).
Uniprot: Q9UHB9
RRID: AB_2845037

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Zebrafish(82%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(91%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
SRP68 Antibody detects endogenous levels of total SRP68.
RRID:
AB_2845037
Cite Format: Affinity Biosciences Cat# DF12232, RRID:AB_2845037.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Signal recognition particle 68; Signal recognition particle 68 kDa; Signal recognition particle 68 kDa protein; signal recognition particle 68kD; Signal recognition particle 68kDa; signal recognition particle subunit SRP68; SRP 68; SRP68; SRP68_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9UHB9(SRP68_HUMAN) >>Visit HPA database.

Gene(ID):
SRP68(6730)
Sequence:
MAAEKQVPGGGGGGGSGGGGGSGGGGSGGGRGAGGEENKENERPSAGSKANKEFGDSLSLEILQIIKESQQQHGLRHGDFQRYRGYCSRRQRRLRKTLNFKMGNRHKFTGKKVTEELLTDNRYLLLVLMDAERAWSYAMQLKQEANTEPRKRFHLLSRLRKAVKHAEELERLCESNRVDAKTKLEAQAYTAYLSGMLRFEHQEWKAAIEAFNKCKTIYEKLASAFTEEQAVLYNQRVEEISPNIRYCAYNIGDQSAINELMQMRLRSGGTEGLLAEKLEALITQTRAKQAATMSEVEWRGRTVPVKIDKVRIFLLGLADNEAAIVQAESEETKERLFESMLSECRDAIQVVREELKPDQKQRDYILEGEPGKVSNLQYLHSYLTYIKLSTAIKRNENMAKGLQRALLQQQPEDDSKRSPRPQDLIRLYDIILQNLVELLQLPGLEEDKAFQKEIGLKTLVFKAYRCFFIAQSYVLVKKWSEALVLYDRVLKYANEVNSDAGAFKNSLKDLPDVQELITQVRSEKCSLQAAAILDANDAHQTETSSSQVKDNKPLVERFETFCLDPSLVTKQANLVHFPPGFQPIPCKPLFFDLALNHVAFPPLEDKLEQKTKSGLTGYIKGIFGFRS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Pig
91
Rabbit
91
Zebrafish
82
Xenopus
73
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHB9 As Substrate

Site PTM Type Enzyme
K5 Acetylation
S16 Phosphorylation
S22 Phosphorylation
S27 Phosphorylation
R31 Methylation
K39 Ubiquitination
S48 Phosphorylation
K49 Ubiquitination
K52 Ubiquitination
K67 Ubiquitination
K112 Ubiquitination
K142 Ubiquitination
K164 Ubiquitination
K205 Ubiquitination
K220 Ubiquitination
S241 Phosphorylation
S267 Phosphorylation
T270 Phosphorylation
K277 Ubiquitination
K288 Ubiquitination
S294 Phosphorylation
K306 Ubiquitination
K333 Ubiquitination
S339 Phosphorylation
K360 Ubiquitination
Y364 Phosphorylation
K372 Ubiquitination
Y385 Phosphorylation
K400 Ubiquitination
K416 Acetylation
K416 Ubiquitination
K452 Acetylation
K452 Ubiquitination
K457 Ubiquitination
K491 Ubiquitination
K504 Ubiquitination
K508 Ubiquitination
T518 Phosphorylation
K524 Ubiquitination
T543 Phosphorylation
C562 S-Nitrosylation
T611 Phosphorylation
K612 Ubiquitination
Y618 Phosphorylation
S627 Phosphorylation

Research Backgrounds

Function:

Signal-recognition-particle assembly has a crucial role in targeting secretory proteins to the rough endoplasmic reticulum membrane. SRP68 binds the 7S RNA, SRP72 binds to this complex subsequently. This ribonucleoprotein complex might interact directly with the docking protein in the ER membrane and possibly participate in the elongation arrest function.

Subcellular Location:

Cytoplasm. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Signal recognition particle consists of a 7S RNA molecule of 300 nucleotides and six protein subunits: SRP72, SRP68, SRP54, SRP19, SRP14 and SRP9.

Family&Domains:

The RNA-binding domain is located near the N-terminus.

Belongs to the SRP68 family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein export.

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