SEPT9 Antibody - #DF12229

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Product: SEPT9 Antibody
Catalog: DF12229
Description: Rabbit polyclonal antibody to SEPT9
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
Mol.Wt.: 65 kDa,38-40 kDa; 65kD(Calculated).
Uniprot: Q9UHD8
RRID: AB_2845034

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(92%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
SEPT9 Antibody detects endogenous levels of total SEPT9.
RRID:
AB_2845034
Cite Format: Affinity Biosciences Cat# DF12229, RRID:AB_2845034.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

AF17q25; Cell division control protein septin D1; KIAA0991; MLL septin like fusion protein; MLL septin-like fusion protein; MLL septin-like fusion protein MSF-A; MSF; MSF1; NAPB; Ov/Br septin; Ovarian/breast septin alpha; Ovarian/Breast septin; PNUTL4; SEPT9; SEPT9_HUMAN; SeptD1; Septin 9; Septin D1; Septin-9; SINT1;

Immunogens

Immunogen:
Uniprot:

Q9UHD8(SEPT9_HUMAN) >>Visit HPA database.

Gene(ID):
SEPT9(10801)
Expression:
Q9UHD8 SEPT9_HUMAN:

Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.

Sequence:
MKKSYSGGTRTSSGRLRRLGDSSGPALKRSFEVEEVETPNSTPPRRVQTPLLRATVASSTQKFQDLGVKNSEPSARHVDSLSQRSPKASLRRVELSGPKAAEPVSRRTELSIDISSKQVENAGAIGPSRFGLKRAEVLGHKTPEPAPRRTEITIVKPQESAHRRMEPPASKVPEVPTAPATDAAPKRVEIQMPKPAEAPTAPSPAQTLENSEPAPVSQLQSRLEPKPQPPVAEATPRSQEATEAAPSCVGDMADTPRDAGLKQAPASRNEKAPVDFGYVGIDSILEQMRRKAMKQGFEFNIMVVGQSGLGKSTLINTLFKSKISRKSVQPTSEERIPKTIEIKSITHDIEEKGVRMKLTVIDTPGFGDHINNENCWQPIMKFINDQYEKYLQEEVNINRKKRIPDTRVHCCLYFIPATGHSLRPLDIEFMKRLSKVVNIVPVIAKADTLTLEERVHFKQRITADLLSNGIDVYPQKEFDEDSEDRLVNEKFREMIPFAVVGSDHEYQVNGKRILGRKTKWGTIEVENTTHCEFAYLRDLLIRTHMQNIKDITSSIHFEAYRVKRLNEGSSAMANGMEEKEPEAPEM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
92
Chicken
92
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UHD8 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K2 Methylation
K3 Methylation
S6 Phosphorylation
R10 Methylation
T11 Phosphorylation
S12 Phosphorylation
S13 Phosphorylation
S22 Phosphorylation
K28 Acetylation
K28 Methylation
K28 Ubiquitination
S30 Phosphorylation
T38 Phosphorylation
S41 Phosphorylation
T42 Phosphorylation
T49 Phosphorylation
S59 Phosphorylation
T60 Phosphorylation
K62 Acetylation
K62 Ubiquitination
S71 Phosphorylation
S80 Phosphorylation
S82 Phosphorylation
S85 Phosphorylation
S89 Phosphorylation
S96 Phosphorylation
S111 Phosphorylation
S115 Phosphorylation
K141 Ubiquitination
T142 Phosphorylation
S160 Phosphorylation
K186 Ubiquitination
S203 Phosphorylation
S211 Phosphorylation
S217 Phosphorylation
T235 Phosphorylation
S238 Phosphorylation
T242 Phosphorylation
S247 Phosphorylation
C248 S-Nitrosylation
T255 Phosphorylation
K262 Ubiquitination
K271 Ubiquitination
Y278 Phosphorylation
S283 Phosphorylation
K291 Ubiquitination
T317 Phosphorylation
K320 Methylation
K320 Ubiquitination
K322 Methylation
K326 Ubiquitination
S327 Phosphorylation
T331 Phosphorylation
S332 Phosphorylation
K338 Ubiquitination
K343 Ubiquitination
K352 Acetylation
K352 Ubiquitination
K357 Ubiquitination
T363 Phosphorylation
K381 Ubiquitination
K389 Ubiquitination
K400 Ubiquitination
K431 Acetylation
K431 Ubiquitination
K435 Ubiquitination
K445 Ubiquitination
T448 Phosphorylation
K458 Ubiquitination
K476 Acetylation
K476 Ubiquitination
S482 Phosphorylation
R485 Methylation
K490 Ubiquitination
S502 Phosphorylation
Y506 Phosphorylation
K511 Acetylation
K511 Ubiquitination
K519 Ubiquitination
T543 Phosphorylation
K549 Ubiquitination

Research Backgrounds

Function:

Filament-forming cytoskeletal GTPase (By similarity). May play a role in cytokinesis (Potential). May play a role in the internalization of 2 intracellular microbial pathogens, Listeria monocytogenes and Shigella flexneri.

Subcellular Location:

Cytoplasm>Cytoskeleton.
Note: In an epithelial cell line, concentrates at cell-cell contact areas. After TGF-beta1 treatment and induction of epithelial to mesenchymal transition, colocalizes partly with actin stress fibers. During bacterial infection, displays a collar shape structure next to actin at the pole of invading bacteria.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed. Isoforms are differentially expressed in testes, kidney, liver heart, spleen, brain, peripheral blood leukocytes, skeletal muscle and kidney. Specific isoforms appear to demonstrate tissue specificity. Isoform 5 is the most highly expressed in fetal tissue. Isoform 1 is detected in all tissues except the brain and thymus, while isoform 2, isoform 3, and isoform 4 are detected at low levels in approximately half of the fetal tissues.

Subunit Structure:

Septins polymerize into heterooligomeric protein complexes that form filaments, and associate with cellular membranes, actin filaments, and microtubules. GTPase activity is required for filament formation. Interacts with SEPTIN2, SEPTIN6, SEPTIN7, SEPTIN11 and SEPTIN14. Interacts with RTKN and ARHGEF18. In a mesenchymal cell line, Rho/RTKN signals cause disruption of wild-type septin filaments, but not of those containing isoform 2 variants HNA Trp-106 and Phe-111. In a mesenchymal cell line, isoform 2 variants HNA Trp-106 and Phe-111, but not wild type, form filaments with SEPTIN4.

Family&Domains:

Belongs to the TRAFAC class TrmE-Era-EngA-EngB-Septin-like GTPase superfamily. Septin GTPase family.

Research Fields

· Human Diseases > Infectious diseases: Bacterial > Bacterial invasion of epithelial cells.

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