Product: RCN2 Antibody
Catalog: DF12226
Description: Rabbit polyclonal antibody to RCN2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 55 kDa; 37kD(Calculated).
Uniprot: Q14257
RRID: AB_2845031

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 100ul $280 In stock
 200ul $350 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(92%)
Clonality:
Polyclonal
Specificity:
RCN2 Antibody detects endogenous levels of total RCN2.
RRID:
AB_2845031
Cite Format: Affinity Biosciences Cat# DF12226, RRID:AB_2845031.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Calcium binding protein ERC 55; Calcium-binding protein ERC-55; E6 binding protein; E6-binding protein; E6BP; ERC 55; ERC55; Rcn2; RCN2_HUMAN; Reticulocalbin 2 EF hand calcium binding domain (endoplasmic reticulum calcium binding protein 55kD); Reticulocalbin 2, EF hand calcium binding domain; Reticulocalbin-2; TCBP49;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q14257 RCN2_HUMAN:

Ubiquitous.

Sequence:
MRLGPRTAALGLLLLCAAAAGAGKAEELHYPLGERRSDYDREALLGVQEDVDEYVKLGHEEQQKRLQAIIKKIDLDSDGFLTESELSSWIQMSFKHYAMQEAKQQFVEYDKNSDDTVTWDEYNIQMYDRVIDFDENTALDDAEEESFRKLHLKDKKRFEKANQDSGPGLSLEEFIAFEHPEEVDYMTEFVIQEALEEHDKNGDGFVSLEEFLGDYRWDPTANEDPEWILVEKDRFVNDYDKDNDGRLDPQELLPWVVPNNQGIAQEEALHLIDEMDLNGDKKLSEEEILENPDLFLTSEATDYGRQLHDDYFYHDEL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
92
Zebrafish
91
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q14257 As Substrate

Site PTM Type Enzyme
T7 Phosphorylation
Y30 Phosphorylation
S37 Phosphorylation
Y54 Phosphorylation
K56 Ubiquitination
K64 Ubiquitination
K71 Ubiquitination
K103 Ubiquitination
T137 Phosphorylation
S146 Phosphorylation
K149 Ubiquitination
S207 Phosphorylation
K232 Ubiquitination
K282 Ubiquitination
Y311 Phosphorylation

Research Backgrounds

Function:

Not known. Binds calcium.

Subcellular Location:

Endoplasmic reticulum lumen.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous.

Family&Domains:

Belongs to the CREC family.

Restrictive clause

 

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For Research Use Only.
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