P4HA1 Antibody | Affinity Biosciences

WB
IHC
IF/ICC

1/8

Western blot analysis of extracts from rat skin tissue, using P4HA1 antibody.

Western blot analysis of extracts from Hela, using P4HA1 Antibody.

DF12219 at 1/100 staining Mouse muscle tissue by IHC-P.

DF12219 at 1/100 staining Rat spleen tissue by IHC-P.

DF12219 at 1/100 staining Human colorectal cancer by IHC-P.

DF12219 at 1/100 staining Rat testis tissue by IHC-P.

DF12219 staining Hela cells by IF/ICC.

Product:	P4HA1 Antibody
Catalog:	DF12219
Description:	Rabbit polyclonal antibody to P4HA1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.:	61 kDa; 61kD(Calculated).
Uniprot:	P13674
RRID:	AB_2845024

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%)

Clonality:

Polyclonal

Specificity:

P4HA1 Antibody detects endogenous levels of total P4HA1.

RRID:

AB_2845024
Cite Format: Affinity Biosciences Cat# DF12219, RRID:AB_2845024.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

2-oxoglutarate-4-dioxygenase subunit alpha-1; 4-hydroxylase, alpha I subunit; 4-PH alpha-1; P4HA; P4HA1; P4HA1_HUMAN; Procollagen-proline; Procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), alpha polypeptide I; Prolyl 4-hydroxylase subunit alpha-1;

Immunogens

Immunogen:

Uniprot:

P13674(P4HA1_HUMAN) >>Visit HPA database.

Gene(ID):

P4HA1(5033)

Sequence:

P13674 P4HA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MIWYILIIGILLPQSLAHPGFFTSIGQMTDLIHTEKDLVTSLKDYIKAEEDKLEQIKKWAEKLDRLTSTATKDPEGFVGHPVNAFKLMKRLNTEWSELENLVLKDMSDGFISNLTIQRQYFPNDEDQVGAAKALLRLQDTYNLDTDTISKGNLPGVKHKSFLTAEDCFELGKVAYTEADYYHTELWMEQALRQLDEGEISTIDKVSVLDYLSYAVYQQGDLDKALLLTKKLLELDPEHQRANGNLKYFEYIMAKEKDVNKSASDDQSDQKTTPKKKGVAVDYLPERQKYEMLCRGEGIKMTPRRQKKLFCRYHDGNRNPKFILAPAKQEDEWDKPRIIRFHDIISDAEIEIVKDLAKPRLRRATISNPITGDLETVHYRISKSAWLSGYENPVVSRINMRIQDLTGLDVSTAEELQVANYGVGGQYEPHFDFARKDEPDAFKELGTGNRIATWLFYMSDVSAGGATVFPEVGASVWPKKGTAVFWYNLFASGEGDYSTRHAACPVLVGNKWVSNKWLHERGQEFRRPCTLSELE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Chicken

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P13674 As Substrate

P13674

Site	PTM Type	Source
T40	Phosphorylation	Uniprot
S41	Phosphorylation	Uniprot
K43	Ubiquitination	Uniprot
Y45	Phosphorylation	Uniprot
K47	Ubiquitination	Uniprot
K62	Ubiquitination	Uniprot
K72	Ubiquitination	Uniprot
N113	N-Glycosylation	Uniprot
K132	Ubiquitination	Uniprot
K150	Ubiquitination	Uniprot
K230	Ubiquitination	Uniprot
Y282	Phosphorylation	Uniprot
K288	Ubiquitination	Uniprot
K299	Ubiquitination	Uniprot
K327	Ubiquitination	Uniprot
S345	Phosphorylation	Uniprot
K353	Ubiquitination	Uniprot
S366	Phosphorylation	Uniprot
K382	Acetylation	Uniprot
K382	Ubiquitination	Uniprot
S387	Phosphorylation	Uniprot
K442	Ubiquitination	Uniprot
K510	Ubiquitination	Uniprot
K515	Ubiquitination	Uniprot

Research Backgrounds

P13674_P4HA1_HUMAN

Function:

Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins.

Subcellular Location:

Endoplasmic reticulum lumen.

Subunit Structure:

Heterotetramer of two alpha-1 chains and two beta chains (the beta chain is the multi-functional PDI).

Family&Domains:

Belongs to the P4HA family.

Research Fields

· Metabolism > Amino acid metabolism > Arginine and proline metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

P4HA1 Antibody - #DF12219