NOB1 Antibody - #DF12216

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Product: NOB1 Antibody
Catalog: DF12216
Description: Rabbit polyclonal antibody to NOB1
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 47-50 kDa; 47kD(Calculated).
Uniprot: Q9ULX3
RRID: AB_2845021

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
NOB1 Antibody detects endogenous levels of total NOB1.
RRID:
AB_2845021
Cite Format: Affinity Biosciences Cat# DF12216, RRID:AB_2845021.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Adenocarcinoma antigen recognized by T lymphocytes 4; ART 4; ART4; MST158; MSTP158; Nin one binding protein; NIN1/RPN12 binding protein 1 homolog (S. cerevisiae); nob1; NOB1_HUMAN; NOB1P; Phosphorylation regulatory protein HP-10; Protein ART-4; Protein ART4; PSMD8 binding protein 1; PSMD8BP1; RNA binding protein NOB1; RNA-binding protein NOB1;

Immunogens

Immunogen:
Uniprot:

Q9ULX3(NOB1_HUMAN) >>Visit HPA database.

Gene(ID):
NOB1(28987)
Expression:
Q9ULX3 NOB1_HUMAN:

Detected in liver, lung, placenta, endothelial cells and spleen.

Sequence:
MAPVEHVVADAGAFLRHAALQDIGKNIYTIREVVTEIRDKATRRRLAVLPYELRFKEPLPEYVRLVTEFSKKTGDYPSLSATDIQVLALTYQLEAEFVGVSHLKQEPQKVKVSSSIQHPETPLHISGFHLPYKPKPPQETEKGHSACEPENLEFSSFMFWRNPLPNIDHELQELLIDRGEDVPSEEEEEEENGFEDRKDDSDDDGGGWITPSNIKQIQQELEQCDVPEDVRVGCLTTDFAMQNVLLQMGLHVLAVNGMLIREARSYILRCHGCFKTTSDMSRVFCSHCGNKTLKKVSVTVSDDGTLHMHFSRNPKVLNPRGLRYSLPTPKGGKYAINPHLTEDQRFPQLRLSQKARQKTNVFAPDYIAGVSPFVENDISSRSATLQVRDSTLGAGRRRLNPNASRKKFVKKR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Zebrafish
83
Chicken
83
Xenopus
82
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9ULX3 As Substrate

Site PTM Type Enzyme
K25 Ubiquitination
T35 Phosphorylation
K40 Ubiquitination
T42 Phosphorylation
K56 Ubiquitination
Y62 Phosphorylation
K71 Ubiquitination
K72 Ubiquitination
K111 Ubiquitination
T121 Phosphorylation P24941 (CDK2)
K133 Ubiquitination
K135 Ubiquitination
T140 Phosphorylation
S184 Phosphorylation
S201 Phosphorylation
T210 Phosphorylation
K275 Methylation
K275 Ubiquitination
S286 Phosphorylation
K291 Ubiquitination
T292 Phosphorylation
K315 Ubiquitination
Y324 Phosphorylation
S325 Phosphorylation
T328 Phosphorylation
K330 Ubiquitination
K333 Acetylation
K333 Ubiquitination
Y334 Phosphorylation
T341 Phosphorylation
R345 Methylation
S352 Phosphorylation
K358 Ubiquitination
T359 Phosphorylation
Y366 Phosphorylation
S390 Phosphorylation
R396 Methylation
S404 Phosphorylation

Research Backgrounds

Function:

May play a role in mRNA degradation (Probable). Endonuclease required for processing of 20S pre-rRNA precursor and biogenesis of 40S ribosomal subunits (By similarity).

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in liver, lung, placenta, endothelial cells and spleen.

Subunit Structure:

Interacts with UPF2 (Probable). Component of the small ribosomal subunit, ribosomal RNA processing complex (SSU RRP complex).

Family&Domains:

Belongs to the NOB1 family.

Research Fields

· Genetic Information Processing > Translation > Ribosome biogenesis in eukaryotes.

References

1). Overexpression of microRNA-107 suppressed proliferation, migration, invasion, and the PI3K/Akt signaling pathway and induced apoptosis by targeting Nin one binding (NOB1) protein in a hypopharyngeal squamous cell carcinoma cell line (FaDu). Bioengineered (PubMed: 35294329) [IF=4.9]

Application: WB    Species: Human    Sample: FaDu cells

Figure 6. NOB1 was a target gene of miR-107. (a) MiR-107 target site in the NOB1 3’ UTR WT was predicted. (b) The dual luciferase reporter assay was employed to demonstrate that NOB1 was a target gene of miR-107. (c) FaDu cells were transfected with miR-107 mimics or mimics NC for 48 h, and the protein expression of NOB1 was detected. Data were expressed as means ± SD (n = 3). **P < 0.01 versus the NOB1 3’ UTR MT+miR-107 mimics group. WT, wild type; MT, mutant type.

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