Patched/PTCH1 Antibody | Affinity Biosciences

1/1

AF0237 at 1/100 staining human kidney tissue sections by IHC-P.

Product:	Patched/PTCH1 Antibody
Catalog:	AF0237
Description:	Rabbit polyclonal antibody to Patched/PTCH1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Mol.Wt.:	160kDa; 161kD(Calculated).
Uniprot:	Q13635
RRID:	AB_2833412

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Clonality:

Polyclonal

Specificity:

Patched/PTCH1 Antibody detects endogenous levels of total Patched/PTCH1.

RRID:

AB_2833412
Cite Format: Affinity Biosciences Cat# AF0237, RRID:AB_2833412.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

A230106A15Rik; BCNS; FLJ26746; FLJ42602; Holoprosencephaly 7; HPE7; mes; NBCCS; OTTHUMP00000021709; OTTHUMP00000021710; Patched; Patched (Drosophila) homolog; Patched 1; Patched homolog (Drosophila); Patched homolog 1 (Drosophila); Patched homolog 1; Patched protein homolog 1; Protein patched homolog 1; PTC; PTC1; PTC1_HUMAN; PTCH; PTCH protein +12b; PTCH protein +4'; PTCH protein -10; PTCH protein; ptch1; PTCH1 protein; PTCH11;

Immunogens

Immunogen:

Uniprot:

Q13635(PTC1_HUMAN) >>Visit HPA database.

Gene(ID):

PTCH1(5727)

Expression:

Q13635 PTC1_HUMAN:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Description:

PTCH1 a multi-pass membrane protein member of the patched family that acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog protein s signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. Interacts with SNX17. Expressed in tumor cells but not in normal skin.

Sequence:

Q13635 PTC1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MASAGNAAEPQDRGGGGSGCIGAPGRPAGGGRRRRTGGLRRAAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGCYIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTTEALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKPPLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDMALVLNGGCHGLSRKYMHWQEELIVGGTVKNSTGKLVSAHALQTMFQLMTPKQMYEHFKGYEYVSHINWNEDKAAAILEAWQRTYVEVVHQSVAQNSTQKVLSFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIGISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRAFSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQAYTDTHDNTRYSPPPPYSSHSFAHETQITMQSTVQLRTEYDPHTHVYYTTAEPRSEISVQPVTVTQDTLSCQSPESTSSTRDLLSQFSDSSLHCLEPPCTKWTLSSFAEKHYAPFLLKPKAKVVVIFLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHRSFSNVKYVMLEENKQLPKMWLHYFRDWLQGLQDAFDSDWETGKIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRLVDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDFVEAIEKVRTICSNYTSLGLSSYPNGYPFLFWEQYIGLRHWLLLFISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVELFGMMGLIGIKLSAVPVVILIASVGIGVEFTVHVALAFLTAIGDKNRRAVLALEHMFAPVLDGAVSTLLGVLMLAGSEFDFIVRYFFAVLAILTILGVLNGLVLLPVLLSFFGPYPEVSPANGLNRLPTPSPEPPPSVVRFAMPPGHTHSGSDSSDSEYSSQTTVSGLSEELRHYEAQQGAGGPAHQVIVEATENPVFAHSTVVHPESRHHPPSNPRQQPHLDSGSLPPGRQGQQPRRDPPREGLWPPPYRPRRDAFEISTEGHSGPSNRARWGPRGARSHNPRNPASTAMGSSVPGYCQPITTVTASASVTVAVHPPPVPGPGRNPRGGLCPGYPETDHGLFEDPHVPFHVRCERRDSKVEVIELQDVECEERPRGSSSN

PTMs - Q13635 As Substrate

Q13635

Site	PTM Type	Source
	Ubiquitination	Uniprot
K163	Ubiquitination	Uniprot
T368	Phosphorylation	Uniprot
S435	Phosphorylation	Uniprot
S463	Phosphorylation	Uniprot
K993	Ubiquitination	Uniprot
T1195	Phosphorylation	Uniprot
S1197	Phosphorylation	Uniprot
S1331	Phosphorylation	Uniprot
R1394	Methylation	Uniprot
S1425	Phosphorylation	Uniprot

Research Backgrounds

Q13635_PTC1_HUMAN

Function:

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

PTMs:

Glycosylation is necessary for SHH binding.

In the absence of Hh ligands, ubiquitination by ITCH at Lys-1426 promotes endocytosis and both proteasomal and lysosomal degradation.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Tissue Specificity:

In the adult, expressed in brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. Expressed in tumor cells but not in normal skin.

Subunit Structure:

Interacts with SNX17. Interacts with IHH. Interacts with G-protein coupled receptor GPR37L1 (By similarity).

Family&Domains:

Belongs to the patched family.

Research Fields

· Environmental Information Processing > Signal transduction > cAMP signaling pathway. (View pathway)

· Environmental Information Processing > Signal transduction > Hedgehog signaling pathway. (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer. (View pathway)

· Human Diseases > Cancers: Overview > Proteoglycans in cancer.

· Human Diseases > Cancers: Specific types > Basal cell carcinoma. (View pathway)

· Organismal Systems > Development > Axon guidance. (View pathway)

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For Research Use Only.
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Patched/PTCH1 Antibody - #AF0237